Involvement of organic anion transporter OAT1 on the chemo-sensitivity in renal cancer cells

Research Project

Project/Area Number	22700886
Research Category	Grant-in-Aid for Young Scientists (B)
Allocation Type	Single-year Grants
Research Field	Tumor biology
Research Institution	Tokyo University of Agriculture and Technology
Principal Investigator	SUZUKI Eriko 東京農工大学, (連合)農学研究科(研究院), 助教 (00468513)
Project Period (FY)	2010-04-01 – 2014-03-31
Project Status	Completed (Fiscal Year 2013)
Budget Amount *help	¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000) Fiscal Year 2013: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000) Fiscal Year 2012: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000) Fiscal Year 2011: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000) Fiscal Year 2010: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Keywords	がん細胞の特性 / 薬剤感受性 / トランスポーター / 化学療法 / 腎癌 / SLCトランスポーター
Research Abstract	Kidney cancer cells are usually resistant to chemotherapy, therefore chemotherapy has not been a standard treatment for kidney cancer. We focused on organic aniontransporter OAT1 expressed in renal glomerular endothelial cells and involved in resorption of general drug. Our study demonstrated that OAT1 expressing kidney cancer cells exhibits increased cell death concomitant with increased incorporation of anionic cancer drug including vincristine. In vivo imaging using EGFP-OAT1 overexpressing cells was first planned, however it was abandoned due to the absense of appropriate image-capturing system.