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Understanding the molecular mechanisms of breast cancer by targeting mutant BRCA1 in hereditary breast cancer cells

Research Project

Project/Area Number 22700888
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Tumor biology
Research InstitutionAichi Medical University

Principal Investigator

SIVASUNDARAM Karnan  愛知医科大学, 医学部, 助教 (30557096)

Project Period (FY) 2010 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2011: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2010: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywordsがん制御遺伝子 / ヒト細胞遺伝子改変法 / BRCA1 / ヒト細胞遺伝子改変
Research Abstract

In this study, we sought to generate experimental platforms that help to understand the mechanism of how a mutation of BRCA1 tumor suppressor gene leads to the onset of human hereditary breast cancer. To this end, we used the technology of human somatic cell gene targeting in an attempt to perform the targeted correction of BRCA1 mutations in 2 cell lines derived from human hereditary breast cancers harboring homozygous BRCA1 mutations, as well as the targeted knock-in of 2 missense mutations into an immortalized human breast epithelial cell line MCF-10A which carries wild-type BRCA1.

Report

(3 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report
  • Research Products

    (13 results)

All 2011 2010

All Journal Article (6 results) (of which Peer Reviewed: 6 results) Presentation (7 results)

  • [Journal Article] Mutation of a single allele of the cancer susceptibility gene BRCA1 leads to genomic instability in human breast epithelial cells.2011

    • Author(s)
      Konishi H, Mohseni M, Tamaki A, Garay JP, Croessmann S, Karnan S, Ota A, Wong HY, Konishi Y, Karakas B, Tahir K, Abukhdeir AM, Gustin JP, Cidado J, Wang GM, Cosgrove D, Cochran R, Jelovac D, Higgins MJ, Arena S, Hawkins L, Lauring J, Gross AL, Heaphy CM, Hosokawa Y, Gabrielson E, Meeker AK, Visvanathan K, Argani P, Bachman KE, Park BH.
    • Journal Title

      Proc Natl Acad Sci U S A

      Volume: 108 Issue: 43 Pages: 17773-8

    • DOI

      10.1073/pnas.1110969108

    • Related Report
      2011 Annual Research Report 2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Genomic profiling combined with gene expression profiling in primary central nervous system lymphoma2011

    • Author(s)
      Sung CO, Kim SC, Karnan S, Karube K, Shin HJ, Nam DH, Suh YL, Kim SH, Kim JY, Kim SJ, Kim WS, Seto M, Ko YH
    • Journal Title

      Blood

      Volume: 117 Issue: 4 Pages: 1291-300

    • DOI

      10.1182/blood-2010-07-297861

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Establishment of an adult T-cell leukemia cell line(HU-ATTAK) dependent for proliferation on human umbilical cord vein endothelial cells2011

    • Author(s)
      Kagami Y, Karnan S, Nakagawa A, Oshiro K, Kato H, Koike K, Morishima Y, Seto M
    • Journal Title

      The Open Leukemia Journal

      Volume: 4 Issue: 1 Pages: 1-8

    • DOI

      10.2174/1876816401104010001

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Genomic profiling combined with gene expression profiling in primary central nervous system lymphoma2011

    • Author(s)
      Sung CO
    • Journal Title

      Blood

      Volume: 117巻 Pages: 1291-1300

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] FIL-4/CD40L co-stimulation induces long-term proliferation for CD10-positive germinal center B cell-like diffuse large B-cell lymphomas2010

    • Author(s)
      Kato H, Kagami Y, Nakagawa M, Karnan S, Yatabe Y, Nakamura S, Morishima Y, Seto M
    • Journal Title

      The Open Leukemia Journal

      Volume: 3 Pages: 60-68

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Enteropathy-associated T-cell lymphoma--a clinicopathologic and array comparative genomic hybridization study2010

    • Author(s)
      Ko YH
    • Journal Title

      Human Pathology

      Volume: 41巻 Pages: 1231-1237

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Presentation] A mutation in a single BRCA1 allele leads to genomic instability in human breast epithelial cells2011

    • Author(s)
      Konishi H
    • Organizer
      第70回日本癌学会学術総会
    • Place of Presentation
      名古屋国際会議場(愛知県)
    • Year and Date
      2011-10-04
    • Related Report
      2011 Annual Research Report 2011 Final Research Report
  • [Presentation] ヒト体細胞株におけるPIGA遺伝子を利用した遺伝子ターゲッティング効率定量系の作成2011

    • Author(s)
      シバスンダラン・カルナン
    • Organizer
      第84回日本生化学会大会
    • Place of Presentation
      国立京都国際会館(京都府)
    • Year and Date
      2011-09-24
    • Related Report
      2011 Annual Research Report 2011 Final Research Report
  • [Presentation] BRCA1ヘテロ変異によってヒト乳腺上皮細胞にゲノム不安定性が発生する2011

    • Author(s)
      小西裕之
    • Organizer
      第84回日本生化学会大会
    • Place of Presentation
      国立京都国際会館(京都府)
    • Year and Date
      2011-09-22
    • Related Report
      2011 Annual Research Report 2011 Final Research Report
  • [Presentation] ヒト培養細胞遺伝子改変の新しい技術を用いた癌関連遺伝子の機能解析2010

    • Author(s)
      小西裕之
    • Organizer
      第33回日本分子生物学会年会第83回日本生化学会大会合同大会
    • Place of Presentation
      神戸国際展示場(兵庫県)
    • Year and Date
      2010-12-07
    • Related Report
      2011 Final Research Report
  • [Presentation] ヒト培養細胞遺伝子改変の新しい技術を用いた癌関連遺伝子の機能解析2010

    • Author(s)
      小西裕之
    • Organizer
      第83回日本生化学会大会
    • Place of Presentation
      神戸国際展示場(兵庫県)
    • Year and Date
      2010-12-07
    • Related Report
      2010 Annual Research Report
  • [Presentation] ヒト培養細胞遺伝子改変の新技術を用いた癌関連遺伝子の機能解析2010

    • Author(s)
      小西裕之
    • Organizer
      第69回日本癌学会学術総会
    • Place of Presentation
      大阪国際会議場(大阪府)
    • Year and Date
      2010-09-22
    • Related Report
      2011 Final Research Report
  • [Presentation] ヒト培養細胞遺伝子改変の新技術を用いた癌関連遺伝子の機能解析2010

    • Author(s)
      小西裕之
    • Organizer
      第69回日本癌学会学術総会
    • Place of Presentation
      第69回日本癌学会学術総会
    • Year and Date
      2010-09-22
    • Related Report
      2010 Annual Research Report

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Published: 2010-08-23   Modified: 2016-04-21  

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