Understanding the molecular mechanisms of breast cancer by targeting mutant BRCA1 in hereditary breast cancer cells
Project/Area Number |
22700888
|
Research Category |
Grant-in-Aid for Young Scientists (B)
|
Allocation Type | Single-year Grants |
Research Field |
Tumor biology
|
Research Institution | Aichi Medical University |
Principal Investigator |
|
Project Period (FY) |
2010 – 2011
|
Project Status |
Completed (Fiscal Year 2011)
|
Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2011: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2010: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
|
Keywords | がん制御遺伝子 / ヒト細胞遺伝子改変法 / BRCA1 / ヒト細胞遺伝子改変 |
Research Abstract |
In this study, we sought to generate experimental platforms that help to understand the mechanism of how a mutation of BRCA1 tumor suppressor gene leads to the onset of human hereditary breast cancer. To this end, we used the technology of human somatic cell gene targeting in an attempt to perform the targeted correction of BRCA1 mutations in 2 cell lines derived from human hereditary breast cancers harboring homozygous BRCA1 mutations, as well as the targeted knock-in of 2 missense mutations into an immortalized human breast epithelial cell line MCF-10A which carries wild-type BRCA1.
|
Report
(3 results)
Research Products
(13 results)
-
[Journal Article] Mutation of a single allele of the cancer susceptibility gene BRCA1 leads to genomic instability in human breast epithelial cells.2011
Author(s)
Konishi H, Mohseni M, Tamaki A, Garay JP, Croessmann S, Karnan S, Ota A, Wong HY, Konishi Y, Karakas B, Tahir K, Abukhdeir AM, Gustin JP, Cidado J, Wang GM, Cosgrove D, Cochran R, Jelovac D, Higgins MJ, Arena S, Hawkins L, Lauring J, Gross AL, Heaphy CM, Hosokawa Y, Gabrielson E, Meeker AK, Visvanathan K, Argani P, Bachman KE, Park BH.
-
Journal Title
Proc Natl Acad Sci U S A
Volume: 108
Issue: 43
Pages: 17773-8
DOI
Related Report
Peer Reviewed
-
[Journal Article] Genomic profiling combined with gene expression profiling in primary central nervous system lymphoma2011
Author(s)
Sung CO, Kim SC, Karnan S, Karube K, Shin HJ, Nam DH, Suh YL, Kim SH, Kim JY, Kim SJ, Kim WS, Seto M, Ko YH
-
Journal Title
Blood
Volume: 117
Issue: 4
Pages: 1291-300
DOI
Related Report
Peer Reviewed
-
-
-
-
-
-
-
-
-
-
-