Two-photon imaging analysis of cytotoxic T cell-mediated anti-tumor immune responses

• Project/Area Number: 22700901
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Tumor immunology
• Research Institution: The Institute of Physical and Chemical Research
• Principal Investigator: KITANO Masahiro 独立行政法人理化学研究所, 免疫細胞動態研究ユニット, 研究員 (40549010)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 二光子 / イメージング / 癌免疫

Research Abstract

We have established the intravital multi-photon imaging system for monitoring the cell behavior of antigen-specific cytotoxic T cells and endogenous dendritic cells in living mouse lymph node and tumor. By using this system, we have visualized the entire process of T cell activation through interactions with endogenous dendritic cells, and identified a dendritic cell subset responsible for antigen presentation selectively to cytotoxic T cells. Furthermore, we have found antigen-specific interactions between tumor-infiltrating effector-cytotoxic T cells and tumor-surrounding dendritic cells, suggesting a new mechanism for inducing T cell proliferation and differentiation not only at the secondary lymphoid organs, but also at the effector sites such as tumor lesion.

Research Products

• [Journal Article] Four-dimensional tracking of lymphocyte migration and interactions in lymph nodes by two-photon microscopy (2012)
  • Author(s): Kitano, M. and Okada, T
  • Journal Title: Methods in Enzymology Volume: Vol.506 Pages: 437-454
  • Peer Reviewed
• [Journal Article] FOUR-DIMENSIONAL TRACKING OF LYMPHOCYTE MIGRATION AND INTERACTIONS BY TWO-PHOTON MICROSCOPY (2012)
  • Author(s): Kitano, M., Okada, T.
  • Journal Title: Methods in Enzymology Volume: 506 Pages: 437-454
  • DOI: 10.1016/b978-0-12-391856-7.00047-0
  • Peer Reviewed
• [Journal Article] Bcl6 protein expression shapes pre-germinal center B cell dynamics and follicular helper T cell heterogeneity (2011)
  • Author(s): Kitano, M., Moriyama, S., Ando, Y., Hikida, M., Mori, Y., Kurosaki, T., and Okada, T
  • Journal Title: Immunity Volume: Vol.34 Pages: 961-972
  • Peer Reviewed
• [Journal Article] Bcl6 protein expression shapes pre-germinal center B cell dynamics and follicular helper T cell heterogeneity (2011)
  • Author(s): M.Kitano, S.Moriyama, Y.Ando, M.Hikida, Y.Mori, T.Kurosaki, T.Okada
  • Journal Title: Immunity Volume: 34 Issue: 6 Pages: 961-972
  • DOI: 10.1016/j.immuni.2011.03.025
  • Peer Reviewed
• [Presentation] BCL6 Protein Expression Shapes Pre-Germinal Center B Cell Dynamics and Follicular Helper T Cell Heterogeneity (2011)
  • Author(s): Kitano, M
  • Organizer: ESF-JSPS Frontier Conferences for Young Researchers
  • Place of Presentation: Hulshorst, The Netherlands
• [Presentation] BCL6 Expression Dynamics in the Lymph Node during the Antibody Response (2010)
  • Author(s): Kitano, M
  • Organizer: The 16th Takeda Science Foundation Symposium on Bioscience
  • Place of Presentation: Tokyo, Japan