Comprehensive analysis of the genomic alterations of gastrointestinal cancers

• Project/Area Number: 22700903
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Tumor diagnosis
• Research Institution: Chiba University
• Principal Investigator: TADA Motohisa 千葉大学, 医学部・附属病院, 助教 (00554239)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2010: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: ゲノム解析 / 大腸癌 / 遺伝子異常 / 高密度アレイ / 消化管癌

Research Abstract

We performed genome-wide analysis of 35 colorectal cancer (CC) and 34 gastric cancer (GC) cell lines for areas of DNA copy number changes and loss of heterozygosity (LOH) by the use of high-density single nucleotide polymorphism (SNP) array. The results from the array analysis were verified in 106 cancer tissues (CC ; 64, GC ; 42) by genomic quantitative real-time PCR and LOH analysis. Several never-detected genomic alterations were detected. Moreover, the overall survival of the patients with LOH with high frequency prolonged compared to that of the patients with LOH with low frequency.

Research Products

• [Journal Article] Prognostic significance of genetic alterations detected by high-density single nucleotide polymorphism array in gastric cancer (2010)
  • Author(s): Tada M, Kanai F, Tanaka Y, Sanada M, Nannya Y, Tateishi K, Ohta M, Asaoka Y, Seto M, Imazeki F, Yoshida H, Ogawa S, Yokosuka O, Omata M
  • Journal Title: Cancer Sci Volume: 101(5) Issue: 5 Pages: 1261-9
  • DOI: 10.1111/j.1349-7006.2010.01500.x
  • NAID: 10026592643
  • Peer Reviewed
• [Journal Article] Prognostic significance of genetic alterations detected by high-density single nucleotide polymorphism array in gastric cancer. (2010)
  • Author(s): Tada Mothisa
  • Journal Title: Cancer Science Volume: 101 Pages: 1261-1269
  • NAID: 10026592643
  • Peer Reviewed
• [Presentation] Comprehensive analysis of allelic imbalance by using high-density single nucleotide polymorphism (SNP) array in gastrointestinal cancer (2011)
  • Author(s): Motohisa Tada
  • Organizer: 13^<th>Japanese-German Cancer Workshop Grand Prince Hotel Hiroshima
  • Place of Presentation: Hiroshima, Japan
  • Year and Date: 2011-09-19
• [Presentation] Comprehensive analysis of allelic imbalance by using high-density single nudeotide polymorphism (SNP) array in gastrointestinal cancer (2011)
  • Author(s): Motohisa Tada
  • Organizer: 13^<th> Japanese-German Cancer Workshop
  • Place of Presentation: Grand Prince Hotel Hiroshima, Hiroshima, Japan
  • Year and Date: 2011-09-19