| Project/Area Number |
22700910
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Tumor diagnosis
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| Research Institution | Tokyo Institute of Technology |
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Principal Investigator |
OGURA Shun-ichiro 東京工業大学, フロンティア研究機構, 特任准教授 (90343160)
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| Project Period (FY) |
2010 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2010: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
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| Keywords | がんの個性診断 / バイオマーカー / 5-アミノレブリン酸 / プロトポルフィリンIX / PEPT1 / ABCG2 / ミトコンドリア / 治療効果予測因子 / 好気代謝 |
|---|
| Research Abstract |
Recently, aminolevulinic acid-based photodynamic therapy(ALA-PDT) is being widely used in cancer therapy owing to the tumor-specific accumulation of photosensitizing protoporphyrin IX(PpIX) after the administration of ALA. In the present study, by focusing on genes involved in the porphyrin biosynthesis pathway, we aimed to explore biomarkers that are predictive for the efficacy of ALA-PDT. We found that high expression of the peptide transporter PEPT1(ALA influx transporter) and low expression of the ATP-binding cassette transporter ABCG2(porphyrin efflux transporter) determined ALA-induced PpIX production and cellular photosensitivity in vitro. Thus, it is suggested that PEPT1 and ABCG2 are potential biomarkers to predict the selective porphyrin accumulation after ALA administration.
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