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Development of radiation-sensitizing anti-cancer agents using G2 checkpoint aberogator

Research Project

Project/Area Number 22700913
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Clinical oncology
Research InstitutionAkita University

Principal Investigator

KOIZUMI Yukio  秋田大学, 大学院・医学系研究科, 助教 (80353465)

Project Period (FY) 2010 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2010: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Keywords抗がん物質探索 / ケミカルバイオロジー / 放射線 / 細胞周期 / シグナル伝達 / 癌
Research Abstract

The present study aims at examing the radiation-sensitizing effect of G2 checkpoint abrogators against solid cancers and discovering the new anti-cancer agent which brings both benefits, enhancing the therapeutic efficacy and decreasing adverse effects of cancer radiotherapy. We examined the effects of various G2 checkpoint abrogators on G2 arrest induction and antiproliferative effect by X-ray irradiation against esophageal cancer cell line, TE5. Cell cycle analysis and trypan blue exclusion assay showed that UCN-01 abrogated G2 arrest and potentiated growth inhibition, respectively. Furthermore, Western blotting demonstrated that UCN-01 decreased the protein level of Chk1 kinase activated by X-ray irradiation.

Report

(3 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report
  • Research Products

    (17 results)

All 2012 2011 2010

All Journal Article (5 results) (of which Peer Reviewed: 5 results) Presentation (12 results)

  • [Journal Article] Generation of intracellular single-chain antibodies directed against polypeptide GalNAc-transferase using a yeast two-hybrid system2012

    • Author(s)
      Ma L, Koyota K, Myoen Y, Yamashita T, Yatabe N, Koizumi Y, Aosasa M, Nishimichi N, Matsuda H, Sugiyama T
    • Journal Title

      Biochem. Biophys. Res. Commun.

      Volume: 418 Issue: 4 Pages: 628-633

    • DOI

      10.1016/j.bbrc.2012.01.062

    • Related Report
      2011 Annual Research Report 2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] In vitro prominent bone regeneration by release zinc ion from Zn-modified implant2011

    • Author(s)
      Yusa K, Yamamoto O, Fukuda M, Koyota S, Koizumi Y, Sugiyama T
    • Journal Title

      Biochem. Biophys. Res.

      Volume: 412 Issue: 1 Pages: 273-278

    • DOI

      10.1016/j.snb.2011.08.037

    • Related Report
      2011 Annual Research Report 2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Fibrinolytic Activation Promoted by the Cyclopentapeptide Malformin: Involvement of Cytoskeletal Reorganization2011

    • Author(s)
      Koizumi Y, Fukudome H, Hasumi K.
    • Journal Title

      Biological and Pharmaceutical Bulletin

      Volume: 34 Issue: 9 Pages: 1426-1431

    • DOI

      10.1248/bpb.34.1426

    • NAID

      130001872435

    • ISSN
      0918-6158, 1347-5215
    • Related Report
      2011 Annual Research Report 2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Sexual dimorphism in LEC rat liver: Suppression of carbonic anhydrase III by copper accumulation during hepatocarcinogenesis2011

    • Author(s)
      Kuhara M, Wang J, Flores MJ, Zhiwei Q, Koizumi Y, Koyota S, Taniguchi N, Sugiyama T.
    • Journal Title

      Biomedical Research

      Volume: 32 Issue: 2 Pages: 111-117

    • DOI

      10.2220/biomedres.32.111

    • NAID

      130004470770

    • ISSN
      0388-6107, 1880-313X
    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Sexual dimorphism in LEC rat liver : Suppression of carbonic anhydrase III by copper accumulation during hepatocarcinogenesis2011

    • Author(s)
      Kuhara M, Wang J, Flores MJ, Zhiwei Q, Koizumi Y, Koyota S, Taniguchi N, Sugiyama T
    • Journal Title

      Biomed.Res.

      Volume: 32 Pages: 111-117

    • NAID

      130004470770

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Presentation] Synthesis and biological evaluation of malformin derivatives a fibrinolysis enhancer2012

    • Author(s)
      Koizumi Y, Nagai K, Hasumi K, Koyota S, Sugiyama T
    • Organizer
      The 6th Korea-Japan Chemical Biology Symposium
    • Place of Presentation
      Sapporo
    • Year and Date
      2012-01-27
    • Related Report
      2011 Final Research Report
  • [Presentation] Synthesis and biological evaluation of malformin derivatives as a fibrinolysis enhancer2012

    • Author(s)
      Koizumi Y, et al.
    • Organizer
      The 6th Korea-Japan Chemical Biology Symposium
    • Place of Presentation
      札幌
    • Year and Date
      2012-01-27
    • Related Report
      2011 Annual Research Report
  • [Presentation] カバノアナタケ抽出液によるマクロファージ脂肪滴形成阻害作用2011

    • Author(s)
      小泉幸央, 姜, 新藤道人, 供田洋, 杉山俊博
    • Organizer
      日本農芸化学
    • Place of Presentation
      山形
    • Year and Date
      2011-10-08
    • Related Report
      2011 Final Research Report
  • [Presentation] カバノアナタケ抽出液によるマクロファージ脂肪滴形成阻害作用2011

    • Author(s)
      小泉幸央, 他
    • Organizer
      日本農芸化学会東北支部 第146回大会
    • Place of Presentation
      山形
    • Year and Date
      2011-10-08
    • Related Report
      2011 Annual Research Report
  • [Presentation] 血栓溶解を促進するマルホルミンの構造活性相関2011

    • Author(s)
      小泉幸央, 長井賢一郎, 蓮見惠司, 小代田宗一, 杉山俊博
    • Organizer
      日本農芸化学会2010年度大会
    • Place of Presentation
      京都
    • Year and Date
      2011-03-27
    • Related Report
      2011 Final Research Report
  • [Presentation] 血栓溶解を促進するマルホルミンの構造活性相関2011

    • Author(s)
      小泉幸央、長井賢一郎、蓮見恵司、小代田宗一、杉山俊博
    • Organizer
      日本農芸化学会2011年度大会
    • Place of Presentation
      京都
    • Year and Date
      2011-03-27
    • Related Report
      2010 Annual Research Report
  • [Presentation] Cosmc阻害による乳ガン細胞の増殖・転移抑制2010

    • Author(s)
      明円悠, 小代田宗一, 馬莉, 小泉幸央, 杉山俊博
    • Organizer
      BMB2010(第33回日本分子生物学会年会第83回日本生化学会大会同大会)
    • Place of Presentation
      神戸
    • Year and Date
      2010-12-10
    • Related Report
      2011 Final Research Report
  • [Presentation] Generation and characterization of intracellular single chain fragment variable (scFv) antibodies against ppGalNacTs2010

    • Author(s)
      Ma L、Koyota S、Myoen Y、Koizumi Y、 Sugiyama T
    • Organizer
      BMB2010(第33回日本分子生物学会年会第83回日本生化学会大会合同大会)
    • Place of Presentation
      神戸
    • Year and Date
      2010-12-10
    • Related Report
      2011 Final Research Report
  • [Presentation] マウスエナメル芽細胞前駆細胞と象牙芽細胞前駆細胞の共培養による石灰化促進2010

    • Author(s)
      小代田宗一, 小代田理恵, 堀川彩夏, 牧野晃大, 遊佐和之, 小泉幸央, 杉山俊博
    • Organizer
      BMB2010(第33回日本分子生物学会年会第83回日本生化学会大会合同大会)
    • Place of Presentation
      神戸
    • Year and Date
      2010-12-10
    • Related Report
      2011 Final Research Report
  • [Presentation] Cosmc阻害による乳ガン細胞の増殖・転移抑制2010

    • Author(s)
      明円悠、小代田宗一、馬莉、小泉幸央、杉山俊博
    • Organizer
      第33回日本分子生物学会年会第83回日本生化学会大会合同大会
    • Place of Presentation
      神戸
    • Year and Date
      2010-12-10
    • Related Report
      2010 Annual Research Report
  • [Presentation] Generation and characterization of intracellular single chain fragment variable (scFv) antibodies against ppGalNacTs2010

    • Author(s)
      Ma L, Koyota S, Myoen Y, Koizumi Y, Sugiyama T
    • Organizer
      第33回日本分子生物学会年会第83回日本生化学会大会合同大会
    • Place of Presentation
      神戸
    • Year and Date
      2010-12-10
    • Related Report
      2010 Annual Research Report
  • [Presentation] マウスエナメル芽細胞前駆細胞と象牙芽細胞前駆細胞の共培養による石灰化促進2010

    • Author(s)
      小代田宗一、小代田理恵、堀川彩夏、牧野晃大、遊佐和之、小泉幸央、杉山俊博
    • Organizer
      第33回日本分子生物学会年会第83回日本生化学会大会合同大会
    • Place of Presentation
      神戸
    • Year and Date
      2010-12-10
    • Related Report
      2010 Annual Research Report

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Published: 2010-08-23   Modified: 2016-04-21  

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