• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Development and application of novel methods for diagnosis of phytoplankton physiological status

Research Project

Project/Area Number 22710003
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Environmental dynamic analysis
Research InstitutionThe University of Tokyo

Principal Investigator

SATO Mitsuhide  東京大学, 大学院・農学生命科学研究科, 助教 (60466810)

Project Period (FY) 2010 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2012: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2010: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywords生物海洋 / 植物プランクトン / リン / アルカリフォスファターゼ / 蛍光基質 / 生物海洋学 / 鉄 / アルカリホスファターゼ / 微量金属 / フローサイトメトリー / 蛍光プローブ / 定量PCR
Research Abstract

Various methods to diagnose phytoplankton physiological status were applied to cultured and natural phytoplankton populations and their validity was examined. In this process, some cultures of oceanic pico- and nanophytoplankton were established. Among them, it was confirmed that measurement of phosphate monoesterase and diesterase activities using fluorogenic substrates in combination with a fluorescent probe ELF-97 were very effective in detecting phosphorus limitation of phytoplankton. By applying this method in the North and South Pacific, the map of phosphorus limitation was drawn throughout the Pacific Ocean. From kinetics studies, it was found that phosphate diesters are recycled much slower than monoesters in the surface water of the tropical Pacific, which is an important result for biogeochemical cycles of phosphorus.

Report

(4 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Annual Research Report
  • 2010 Annual Research Report
  • Research Products

    (7 results)

All 2013 2011 Other

All Journal Article (1 results) (of which Peer Reviewed: 1 results) Presentation (6 results)

  • [Journal Article] 太平洋亜熱帯海域におけるピコ・ナノ植物プランクトンサイズ2013

    • Author(s)
      佐藤光秀・橋濱史典・児玉武稔・古谷研
    • Journal Title

      2013年度日本海洋学会春季大会

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Presentation] 太平洋亜熱帯海域におけるピコ・ナノ植物プランクトンサイズ2013

    • Author(s)
      佐藤光秀・橋濱史典・児玉武稔・古谷 研
    • Organizer
      2013年度日本海洋学会春季大会
    • Place of Presentation
      東京
    • Year and Date
      2013-03-22
    • Related Report
      2012 Final Research Report
  • [Presentation] Distributions of alkaline phosphatase and disphosphatase activities in the Pacific Ocean, with an emphasis on phosphorus cycling in subtropical gyres2013

    • Author(s)
      Sato, M., R. Sakuraba, and F. Hashihama
    • Organizer
      ASLO 2013 Aquatic Sciences Meeting
    • Place of Presentation
      New Orleans, USA
    • Year and Date
      2013-02-19
    • Related Report
      2012 Final Research Report
  • [Presentation] Alkaline phosphatase and diphosphatase activities in the Pacific Ocean2013

    • Author(s)
      M. Sato
    • Organizer
      ASLO 2013 Aquatic Sciences Meeting
    • Place of Presentation
      New Orleans (USA)
    • Related Report
      2012 Annual Research Report
  • [Presentation] 太平洋亜熱帯域における植物プランクトンの増殖と制限要因2011

    • Author(s)
      佐藤光秀
    • Organizer
      東京大学大気海洋研究所共同利用研究集会 亜熱帯太平洋のプランクトン生態系および物質循環に関する比較海洋学
    • Place of Presentation
      東京
    • Year and Date
      2011-10-03
    • Related Report
      2012 Final Research Report
  • [Presentation] 太平洋亜熱帯海域における植物プランクトンの増殖と制限要因2011

    • Author(s)
      佐藤光秀
    • Organizer
      東京大学大気海洋研究所共同利用研究集会「亜熱帯太平洋プランクトン生態系および物質循環に関する比較海洋学」
    • Place of Presentation
      東京大学農学部(東京都)(招待講演)
    • Year and Date
      2011-10-03
    • Related Report
      2011 Annual Research Report
  • [Presentation] 太平洋亜熱帯海域におけるピ コ・ナノ植物プランクトンサイズ

    • Author(s)
      佐藤光秀
    • Organizer
      2013年度日本海洋学会春季大会
    • Place of Presentation
      東京海洋大学(東京)
    • Related Report
      2012 Annual Research Report

URL: 

Published: 2010-08-23   Modified: 2019-07-29  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi