DNA analysis of a single pollen grain to identify the provenance of pollen transported to Antarctica

• Project/Area Number: 22710023
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Environmental dynamic analysis
• Research Institution: 大学共同利用機関法人情報・システム研究機構 (2011); 大学共同利用機関法人情報・システム研究機構(新領域融合研究センター、DBCLS) (2010)
• Principal Investigator: NAKAZAWA Fumio 大学共同利用機関法人情報・システム研究機構, 新領域融合研究センター, 融合プロジェクト特任研究員 (80432178)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000); Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2010: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 南極 / 物質循環 / DNA / 花粉 / 氷河 / マツ属 / ロシア / アイスコア / 雪氷 / 環境変動 / 花粉分析 / 気候変動

Research Abstract

This study attempted to develop a method to identify the pollen grain at species level by analyzing its DNA in order to define a provenance of the pollen that can be found in Antarctic snow and ice. For the development, Pinus pollen grains extracted from a glacier in Russia was analyzed as a preliminary approach. Pinus pollen is a major type(> 50%) of pollen in Antarctic snow and ice. Modern pollen analysis observes pollen morphology with a microscope, identification of Pinus pollen is limited to genus level only. This study allowed us to identify the pollen at subsection level that is one rank higher of species.

Research Products

• [Journal Article] Snow density along the route traversed in the Japanese-Swedish Antarctic Expedition 2007/08 (2012)
  • Author(s): Sugiyama, S., H.Enomoto, S.Fujita, K.Fukui, F.Nakazawa, P.Holmlund, S.Surdyk
  • Journal Title: Journal of Glaciology Volume: 58 Issue: 209 Pages: 529-539
  • DOI: 10.3189/2012jog11j201
  • NAID: 120004979503
  • Peer Reviewed
• [Journal Article] The rates of sea salt sulfatization in the atmosphere and surface snow of inland Antarctica (2012)
  • Author(s): Iizuka, Y., A.Tsuchimoto, Y.Hoshina, T.Sakurai, M.Hansson, T.Karlin, K.Fujita, F.Nakazawa, H.Motoyama, S.Fujita
  • Journal Title: J.Geophys.Res. Volume: 117 Issue: D4
  • DOI: 10.1029/2011jd016378
  • NAID: 120004593469
  • Peer Reviewed
• [Journal Article] Spatial and temporal variability of snow accumulation rate on the East Antarctic ice divide between Dome Fuji and EPICA DML (2012)
  • Author(s): Fujita, S., Holmlund, P., Andersson, I., Brown, I., Enomoto, H., Fujii, Y., Fujita, K., Fukui, K., Furukawa, T., Hansson, M., Hara, K., Hoshina, Y., Igarashi, M., Iizuka, Y., Imura, S., Ingvander, S., Karlin, T., Motoyama, H., Nakazawa, F., Oerter, H., Sjoberg, L.E., Sugiyama, S., Surdyk, S., Strom, J., Uemura, R., Wilhelms, F.
  • Journal Title: The Cryosphere Volume: 5 Issue: 4 Pages: 1057-1081
  • DOI: 10.5194/tc-5-1057-2011
  • Peer Reviewed
• [Journal Article] Establishing the timing of chemical deposition events on Belukha glacier, Altai Mountains, Russia, using pollen analysis (2011)
  • Author(s): Nakazawa F., T. Miyake, K. Fujita, N. Takeuchi, J. Uetake, T. Fujiki, V. Aizen and M. Nakawo
  • Journal Title: Arctic, Antarctic, and Alpine Research Volume: 43(1) Pages: 66-72
  • URL: http://dx.doi.org/10.1657/1938-4246-43.1.66.
• [Journal Article] Quantification of Total Mercury in Antarctic Surface Snow using ICP-SF-MS : Spatial Variation from the Coast to Dome Fuji (2011)
  • Author(s): Han Y., Y.Huh, S.Hong, S.D.Hur, H.Motoyama, S.Fujita, F.Nakazawa, K.Fukui
  • Journal Title: Bulletin of the Korean Chemical Society Volume: 32 Pages: 4258-4264
  • Peer Reviewed
• [Journal Article] Establishing the timing of chemical deposition events on Belukha glacier, Altai Mountains, Russia, using pollen analysis (2011)
  • Author(s): Nakazawa F., T.Miyake, K.Fujita, N.Takeuchi, J.Uetake, T.Fujiki, V.Aizen, M.Nakawo
  • Journal Title: Arctic, Antarctic, and Alpine Research Volume: 43 Pages: 66-72
  • Peer Reviewed
• [Journal Article] Evidence for propagation of cold-adapted yeast in an ice core from a Siberian Altai glacier (2011)
  • Author(s): Uetake J., S.Kohshima, F.Nakazawa, N.Takeuchi, K.Fujita, T.Miyake, H.Narita, V.B.Aizenand M.Nakawo
  • Journal Title: Journal of Geophysical Research-Biogeosciences Volume: 116
  • Peer Reviewed
• [Journal Article] Re-evaluation of the reconstruction of summer temperatures from meltfeatures in Belukha ice cores, Siberian Altai (2011)
  • Author(s): Okamoto S, K.Fujita, H.Narita, J.Uetake, N.Takeuchi, T.Miyake, F.Nakazawa, V.Aizen, S.Nikitin, M.Nakawo
  • Journal Title: Journal of Geophysical Research-Atmospheres Volume: 116
  • Peer Reviewed
• [Presentation] 節レベルでの同定を目的としたベルーハ氷河中のマツ属花粉1粒ずつのDNA分析 (2011)
  • Author(s): 中澤文男・植竹淳・陶山佳久・金子亮・竹内望・藤田耕史・本山秀明・神田啓史
  • Organizer: 第2回極域科学シンポジウム
  • Place of Presentation: 国立極地研究所(東京都立川市)
  • Year and Date: 2011-11-16
• [Presentation] 節レベルでの同定を目的とした氷河中のマツ属花粉1粒ずつのDNA分析 (2011)
  • Author(s): 中澤文男・植竹淳・陶山佳久・金子亮・竹内望・藤田耕史・本山秀明・神田啓史
  • Organizer: 日本花粉学会第52回大会
  • Place of Presentation: 名城大学(愛知県名古屋市)
  • Year and Date: 2011-10-09
• [Presentation] 節レベルでの識別を目的としたベルーハ氷河中のマツ属花粉1粒ずつのDNA分析 (2011)
  • Author(s): 中澤文男・植竹淳・陶山佳久・金子亮・竹内望・藤田耕史・本山秀明・神田啓史
  • Organizer: 雪氷研究大会
  • Place of Presentation: ハイブ長岡(新潟県長岡市)
  • Year and Date: 2011-09-21
• [Presentation] 節レベルでの識別を目的としたベルーハ氷河中のマツ属花粉1粒ずつのDNA分析 (2011)
  • Author(s): 中澤文男・植竹淳・陶山佳久・金子亮・竹内望・藤田耕史・本山秀明・神田啓史
  • Organizer: 雪氷研究大会(2011)
  • Place of Presentation: ハイブ長岡(新潟県長岡市)
  • Year and Date: 2011-09-21
• [Presentation] ベルーハ氷河に含まれるマツ属花粉1粒ずつのDNA分析 (2011)
  • Author(s): 中澤文男・植竹淳・陶山佳久・金子亮・竹内望・藤田耕史・本山秀明・神田啓史
  • Organizer: 日本地球惑星科学連合2011年大会
  • Place of Presentation: 幕張メッセ国際会議場(千葉県千葉市)
  • Year and Date: 2011-05-24
• [Presentation] DNA分析による氷河中のマツ属花粉の詳細分類 (2010)
  • Author(s): 中澤文男・植竹淳・陶山佳久・金子亮・竹内望・藤田耕史・本山秀明・神田啓史
  • Organizer: 第32回極域生物シンポジウム
  • Place of Presentation: 国立極地研究所(東京都立川市)
  • Year and Date: 2010-11-30
• [Presentation] DNA分析による氷河試料中のマツ属花粉の分類 (2010)
  • Author(s): 中澤文男・植竹淳・陶山佳久・金子亮・竹内望・竹内望・藤田耕史・本山秀明・神田啓史
  • Organizer: 日本花粉学会第51回大会
  • Place of Presentation: 中央大学後楽園キャンパス(東京都文京区)
  • Year and Date: 2010-10-10
• [Presentation] 氷河試料中のマツ属花粉1粒ずつDNA分析(2) (2010)
  • Author(s): 中澤文男・植竹淳・陶山佳久・金子亮・竹内望・竹内望・藤田耕史・本山秀明・神田啓史
  • Organizer: 雪氷研究大会
  • Place of Presentation: 東京エレクトロンホール宮城(宮城県仙台市)
  • Year and Date: 2010-09-27
• [Presentation] 氷河試料中のマツ属花粉1粒ずつDNA分析(2) (2010)
  • Author(s): 中澤文男・植竹淳・陶山佳久・金子亮・竹内望・竹内望・藤田耕史・本山秀明・神田啓史
  • Organizer: 雪氷研究大会(2010)
  • Place of Presentation: 東京エレクトロンホール宮城(宮城県仙台市)
  • Year and Date: 2010-09-27
• [Presentation] DNA analysis of a single Pinus pollen grain in a glacier for identification of the species (2010)
  • Author(s): Nakazawa F, J. Uetake, Y. Suyama, R. Kaneko, N. Takeuchi, K. Fujita and H. Kanda
  • Organizer: International Symposium on Snow, Ice and Humanity in a Changing Climate
  • Place of Presentation: Hokkaido University(Sapporo、Japan)
  • Year and Date: 2010-06-22
• [Presentation] DNA analysis of a single Pinus pollen grain in a glacier for identification of the species (2010)
  • Author(s): Nakazawa F, J.Uetake, Y.Suyama, R.Kaneko, N.Takeuchi, K.Fujita, H.Kanda
  • Organizer: International Symposium on Snow, Ice and Humanity in a Changing Climate
  • Place of Presentation: Hokkaido University (Sapporo, Japan)
  • Year and Date: 2010-06-22
• [Presentation] ベルーハ氷河における積雪の花粉分析から同定された化学成分の沈着時期 (2010)
  • Author(s): 中澤文男・三宅隆之・藤田耕史・竹内望・植竹淳・藤木利之・ウラジミールアイゼン・中尾正義
  • Organizer: 日本地球惑星科学連合2010年大会
  • Place of Presentation: 幕張メッセ国際会議場(千葉県千葉市)
  • Year and Date: 2010-05-28
• [Presentation] 種レベルでの花粉分析を目的とした氷河中のマツ属花粉1粒ずつのDNA分析(2) (2010)
  • Author(s): 中澤文男・植竹淳・陶山佳久・金子亮・竹内望・藤田耕史・神田啓史
  • Organizer: 日本地球惑星科学連合2010年大会
  • Place of Presentation: 幕張メッセ国際会議場(千葉県千葉市)
  • Year and Date: 2010-05-27