Analysis of a new mechanism on homology-dependent repair

• Project/Area Number: 22710053
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Risk sciences of radiation/Chemicals
• Research Institution: Kyoto University
• Principal Investigator: KATO Akihiro 京都大学, 放射線生物研究センター, 研究員 (70423051)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2010: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
• Keywords: DNA修復 / 相同組換え / ゲノム / 放射線 / 蛋白質 / 核酸 / 細胞・組織

Research Abstract

In order to clarify the biological significance of the physical interaction between NBS1 and RAD51, we tried to establish a NBS1 mutant that was not able to bind to RAD51. In this process, we found that NBS1 interacted with RAD51 through MRE11. The MRE11 mutant that could not interact with RAD51 had full activity of homology-dependent repair of DNA double-strand breaks(DSBs). Cells expressing the MRE11 mutant did not show sensitivity to ionizing radiation. These results indicate that the interaction between NBS1 and RAD51 does not have an important role in DSB repair but has a role in other mechanisms.

Research Products

• [Journal Article] Genistein, isoflavonoids in soybeans, prevents the formation of excess radiation-induced centrosomes via p21up-regulation (2011)
  • Author(s): Shimada M, Kato A, Habu T, Komatsu K
  • Journal Title: Mutatation Research Volume: Volume 716 Issue: 1-2 Pages: 27-32
  • DOI: 10.1016/j.mrfmmm.2011.07.017
  • Peer Reviewed
• [Journal Article] HERC2 Interacts with Claspin and regulates DNA origin firingandr eplication fork progression (2011)
  • Author(s): Izawa N, Wu W, Sato K, Nishikawa H, Kato A, Boku N, Itoh F, Ohta T
  • Journal Title: Cancer Research Volume: Volume 71 Issue: 17 Pages: 5621-5625
  • DOI: 10.1158/0008-5472.can-11-0385
  • Peer Reviewed
• [Journal Article] NBS1 recruits RAD18 via aRAD6-like domain and regulates Polη-dependent translesion DNA synthesis (2011)
  • Author(s): Yanagihara H, Kobayashi J, Tateishi S, Kato A, Matsuura S, Tauchi H, Yamada K, Takezawa J, Sugasawa K, Masutani C, Hanaoka F, Weemaes CM, Mori T, Zou L, Komatsu K.
  • Journal Title: Mol Cell Volume: 43 Issue: 5 Pages: 788-797
  • DOI: 10.1016/j.molcel.2011.07.026
  • Peer Reviewed
• [Journal Article] Regulation of homologous recombination by RNF20-dependent H2Bubiquitination (2011)
  • Author(s): Nakamura K, Kato A, Kobayashi J, Yanagihara H, Sakamoto S, Oliveira D, Shimada S, Tauchi H, Suzuki H, Tashiro S, Zou L, Komatsu K.
  • Journal Title: Mol Cell Volume: 41 Issue: 5 Pages: 515-528
  • DOI: 10.1016/j.molcel.2011.02.002
  • NAID: 130007000484
  • Peer Reviewed
• [Journal Article] Regulation of Homologous Recombination by RNF20-Dependent H2B Ubiquitination (2011)
  • Author(s): 中村恭介、加藤晃弘, et.al
  • Journal Title: Molecular Cell Volume: Volume 41 Pages: 515-528
  • NAID: 130007000484
  • Peer Reviewed
• [Presentation] MRN複合体とRAD51の相互作用 (2011)
  • Author(s): 加藤晃弘
  • Organizer: 日本放射線影響学会第54回大会
  • Place of Presentation: 神戸
  • Year and Date: 2011-11-18
• [Presentation] MRN複合体とRAD51の相互作用 (2011)
  • Author(s): 加藤晃弘
  • Organizer: 日本放射線影響学会第54回大会
  • Place of Presentation: 神戸商工会議所会館(神戸市)
  • Year and Date: 2011-11-18
• [Presentation] Physical and functional interaction between MRN complex and RAD51 (2011)
  • Author(s): 加藤晃弘
  • Organizer: 第70回日本癌学会学術総会
  • Place of Presentation: 名古屋
  • Year and Date: 2011-10-05
• [Presentation] Physical and functional interaction between MRN complex and RAD51 (2011)
  • Author(s): 加藤晃弘
  • Organizer: 第70回日本癌学会学術総会
  • Place of Presentation: 名古屋国際会議場(名古屋市)
  • Year and Date: 2011-10-05
• [Presentation] The link between MRN complex and RAD51 (2011)
  • Author(s): 加藤晃弘
  • Organizer: ICRR2011
  • Place of Presentation: ポーランド
  • Year and Date: 2011-08-30
• [Presentation] The link between MRN complex and RAD51 (2011)
  • Author(s): 加藤晃弘
  • Organizer: ICRR2011
  • Place of Presentation: 京都
  • Year and Date: 2011-08-30
• [Presentation] The link between MRN complex and RAD51 (2011)
  • Author(s): 加藤晃弘
  • Organizer: ICRR2011
  • Place of Presentation: ワルシャワ(ポーランド)
  • Year and Date: 2011-08-30
• [Presentation] MRN複合体とRAD51との関係 (2010)
  • Author(s): 加藤晃弘
  • Organizer: 日本放射線影響学会第53回大会
  • Place of Presentation: 大阪
  • Year and Date: 2010-10-20
• [Presentation] MRN複合体とRAD51との関係 (2010)
  • Author(s): 加藤晃弘
  • Organizer: 日本放射線影響学会第53回大会
  • Place of Presentation: 京都
  • Year and Date: 2010-10-20
• [Presentation] RAD51がMRN複合体に結合する (2010)
  • Author(s): 加藤晃弘
  • Organizer: 第69回日本癌学会学術総会
  • Place of Presentation: 大阪
  • Year and Date: 2010-09-24
• [Presentation] Control of homologous recombination by RNF20-dependent H2B ubiquitination (2010)
  • Author(s): 加藤晃弘
  • Organizer: EMBO workshop「The Interface between the Ubiquitin Family and the DNA Damage Response」
  • Place of Presentation: クロアチア
  • Year and Date: 2010-09-04
• [Presentation] Control of homologous recombination by RNF20-dependent H2B ubiquitination (2010)
  • Author(s): 加藤晃弘
  • Organizer: EMBO workshop 「The Interface between the Ubiquitin Family and the DNA Damage Response」
  • Place of Presentation: クロアチア,ロビーニ
  • Year and Date: 2010-09-04
• [Presentation] Analysis of the relationship between the MRN complex and RAD51. (2010)
  • Author(s): 加藤晃弘
  • Organizer: Maintenance of Genome Stability 2010
  • Place of Presentation: (Antigua and Batbuda) Bolans village
  • Year and Date: 2010-03-10