The role of DNA repair factor FNACD2 in centrosome duplication maintenance

• Project/Area Number: 22710054
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Risk sciences of radiation/Chemicals
• Research Institution: Kyoto University
• Principal Investigator: SHIMADA Mikio 京都大学, 放射線生物研究センター, 研究員 (20548557)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: DNA修復 / 中心体 / 発癌 / FANCD2 / 発癌制御 / 染色体異常

Research Abstract

Centrosome is an organelle function as microtubules organizing center. Disfunction of centrosome results in improper cell division and chromosome unstability leads to tumorigenesis. FANCD2 is a cause gene of Fanconi Anemia, which represent predisposition malignancy. In this study, we addressed the role of FNACD2 in centrosome maintence. Defect of FANCD2 by siRNA knockdown in U2OS cells result in centrosome overduplication and reducing of mictorubule assembly function. These results indicated that FANCD2 is involved in centrosome functions.

Research Products

• [Journal Article] Genistein, isoflavonoids in soybeans, prevents the formation of excess radiation-induced centrosomes via p21up-regulation (2011)
  • Author(s): Shimada M, Kato A, Habu T, Komatsu K
  • Journal Title: Mutatation Research Volume: Volume 716 Issue: 1-2 Pages: 27-32
  • DOI: 10.1016/j.mrfmmm.2011.07.017
  • Peer Reviewed
• [Journal Article] Poteintial role of DNA repiar proteins in centrosome maintenance (2011)
  • Author(s): Shimada M, Kato A, Kobayashi J.
  • Journal Title: Book 4/DNA Pages: 55-66
  • DOI: 10.5772/21396
  • ISBN: 9789533076973
  • Peer Reviewed
• [Journal Article] Regulation of homologous recombination by RNF20-dependent H2Bubiquitination (2011)
  • Author(s): Nakamura K, Kato A, Kobayashi J, Yanagihara H, Sakamoto S, Oliveira D, Shimada S, Tauchi H, Suzuki H, Tashiro S, Zou L, Komatsu K.
  • Journal Title: Mol Cell Volume: 41 Issue: 5 Pages: 515-528
  • DOI: 10.1016/j.molcel.2011.02.002
  • NAID: 130007000484
  • Peer Reviewed
• [Journal Article] The potential roles of DNA repair proteins in centrosome maintenance (2011)
  • Author(s): Mikio shimda, Akihiro Kato, Junya Kobayashi
  • Journal Title: DNA repair/Book Volume: 4 Pages: 57-66
  • Peer Reviewed
• [Journal Article] Regulation of homologous recombination by RNF20-dependent H2B ubiquitination. (2011)
  • Author(s): K.Nakamura, A.Kato, J.Kobayashi, H.Yanagihara, S.Sakamoto, D.V.N.P.Oliveira, M.Shimada, H.Tauchi, H.Suzuki, S.Tashiro, L.Zou, K.Komatsu
  • Journal Title: Molcular Cell Volume: 41 Pages: 515-528
  • NAID: 130007001514
  • Peer Reviewed
• [Journal Article] Differential role of repair proteins, BRCA1/NBS1 and Ku70/DNA-PKcs, in radiation-induced centrosome overduplication (2010)
  • Author(s): Shimada M, Kobayashi J, Hirayama R, Komatsu K
  • Journal Title: Cancer Sci Volume: 101 Pages: 2531-2537
  • NAID: 10027834303
  • Peer Reviewed
• [Journal Article] Differential role of repair proteins, BRCA1/NBS1 and Ku70/DNA-PKc in radiation-induced centrosome over-duplication. (2010)
  • Author(s): M.Shimada, J.Kobayashi, R.Hirayama, K.Komatsu
  • Journal Title: Cancer Science Volume: 101 Pages: 2531-2537
  • Peer Reviewed
• [Presentation] Ionizing radiation induced-microcephaly depends on dissociation of apical structure during embryonic development of the mouse cerebral cortex. (2011)
  • Author(s): M Shimada, JN Puivers, F Matsuzaki, T Matsumoto, K Komatsu
  • Organizer: International Congress of Radiation Research
  • Place of Presentation: Walsaw
  • Year and Date: 2011-08-29
• [Presentation] Ionizing radiation induced-microcephaly depends on dissociation of apical structure during embryonic development of the mouse cerebral cortex (2011)
  • Author(s): M Shimada, JN Puivers, F Matsuzaki, T Matsumoto, K Komatsu
  • Organizer: International Congress of Radiation Research
  • Place of Presentation: ワルシャワ、ポーランド
  • Year and Date: 2011-08-29
• [Presentation] High LET重粒子線照射時の中心体複製への影響の検討 (2011)
  • Author(s): 島田幹男、平山亮一、小松賢
  • Organizer: 平成二十二年度HIMAC共同利用研究成果発表会
  • Place of Presentation: 千葉
  • Year and Date: 2011-04-23
• [Presentation] High LET重粒子線照射時の中心体複製への影響の検討 (2011)
  • Author(s): 島田幹男、平山亮一、小松賢志
  • Organizer: 平成二十三年度HIMAC共同利用研究成果発表会
  • Place of Presentation: 千葉
  • Year and Date: 2011-04-23
• [Presentation] Centrosome maintenance by NBS1/BRCA1 dependent ubiquitination ofγ-tubulin (2011)
  • Author(s): Mikio Shimada and Kenshi Komatsu
  • Organizer: Keystone symposia, Genomic Instability and DNA repair
  • Place of Presentation: Keystone, Colorado
  • Year and Date: 2011-02-03
• [Presentation] NBS1/BRCA1 pathway dependent ubuquitination of gamma-tubulin is involved in centrosome maintenance. (2011)
  • Author(s): Mikio shimada, Kenshi Komatsu
  • Organizer: Keystone Symposia Genomic Instability and DNA Repair.
  • Place of Presentation: アメリカ、コロラド
  • Year and Date: 2011-02-03
• [Presentation] 放射線誘発小頭症における中心体による神経幹細胞制御機構の解析 (2011)
  • Author(s): 島田幹男、松崎文雄、小松賢志
  • Organizer: 第一回放射線神経生物研究集会
  • Place of Presentation: 前橋
  • Year and Date: 2011-01-29
• [Presentation] 放射線誘発小頭症における中心体による神経幹細胞制御機構の解析 (2011)
  • Author(s): 島田幹男、松崎文雄、小松賢志
  • Organizer: 第一回放射線神経生物研究集会
  • Place of Presentation: 群馬
  • Year and Date: 2011-01-29
• [Presentation] Centrosome maintenance by NBS1/BRCA1-dependent ubiquitination ofγ-tubulin (2010)
  • Author(s): Mikio shimada and Kenshi Komatsu
  • Organizer: RBC International Symposium
  • Place of Presentation: Kyoto
  • Year and Date: 2010-10-22
• [Presentation] Centrosome maintenance by NBS1/BRCA1-dependent ubiquitination of γ-tubulin (2010)
  • Author(s): Mikio shimada, Kenshi Komatsu
  • Organizer: RBC International Symposium
  • Place of Presentation: 京都(招待講演)
  • Year and Date: 2010-10-22
• [Presentation] 中心体過剰複製と線量率効果の解析 (2010)
  • Author(s): 島田幹男、小林純也、小松賢志
  • Organizer: 第53回日本放射線影響学会
  • Place of Presentation: 京都
  • Year and Date: 2010-10-21
• [Presentation] Novel role of DNA repair protein FANCD2 in centrosome maintenance (2010)
  • Author(s): 島田幹男、小林純也、小松賢志
  • Organizer: 第67回日本癌学会学術総会
  • Place of Presentation: 大阪
  • Year and Date: 2010-09-22
• [Presentation] Novel role of DNA repair protein FANCD2 in centrosome maintenance (2010)
  • Author(s): 島田幹男、小林純也、小松賢志
  • Organizer: 日本細胞生物学会
  • Place of Presentation: 大阪
  • Year and Date: 2010-05-22
• [Presentation] Novel role of DNA repair protein FANCD2 in centrosome maintenance (2010)
  • Author(s): 島田幹男、小林純也、小松賢志
  • Organizer: 第62回日本細胞生物学会
  • Place of Presentation: 大阪
  • Year and Date: 2010-05-22
• [Presentation] High LET重粒子線照射時の中心体複製への影響の検討 (2010)
  • Author(s): 島田幹男、平山亮一、小松賢志
  • Organizer: 平成二十一年度HIMAC共同利用研究成果発表会
  • Place of Presentation: 千葉
• [Remarks] 研究室web site
  • URL: http://www.rbc.kyoto-u.ac.jp/Genome/index.html
• [Remarks] 研究室web site
  • URL: http://www.rbc.kyoto-u.ac.jp/Genome/index.html