| Project/Area Number |
22710061
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Risk sciences of radiation/Chemicals
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| Research Institution | National Institute of Health Sciences |
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Principal Investigator |
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| Research Collaborator |
HONMA Masamitsu 国立医薬品食品衛生研究所, 変異遺伝部, 室長 (30250179)
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| Project Period (FY) |
2010 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2010: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | 修復 / ゲノム / プロテオーム / DNA修復 / 突然変異 / 放射線 |
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| Research Abstract |
DNA topoisomerase I(Top1) is an essential enzyme involved in resolving the torsional stress associated with DNA replication and transcription. Top1 have a deleterious effect on cells in these processes. In the event, when the final re-ligation step of the reaction cycle is prevented, the covalent topoisomerase I. DNA intermediate becomes a toxic DNA lesion, called topoisomerase I-DNA covalent complex(Top1-cc). These lesions must be repaired. The molecular mechanisms of repair of Top1-cc seem to be complicated with DNA replication, transcription, recombination and protein degradation. However, detailed mechanisms are not clear. Here we purified and analyzed Top1 protein complexes to know molecular mechanisms of repair of Top1-cc, Top1 itself and interaction proteins.
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