| Project/Area Number |
22710062
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Risk sciences of radiation/Chemicals
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| Research Institution | The University of Tokyo |
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Principal Investigator |
KOMORI Kikuo 東京大学, 生産技術研究所, 助教 (60431813)
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| Project Period (FY) |
2009 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2010: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | トキシコロジー / 細胞組織チップ / 毒性評価 / 組織工学 / 3次元組織 / マイクロファブリケーション / 蛍光イメージング |
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| Research Abstract |
In the present work, a three-dimensional micro-tissue of human hepatocarcinoma Hep G2 cells was formed in microwells incorporated in a polydimethylsiloxane sheet and was determined its minimum required size for in vitro toxicity analyses. Based on the EROD assay, the intracellular enzyme cytochrome P450(CYP) 1A1/2 activity was evaluated as an index of liver functions. The drug metabolic activity for the micro-tissue 200μm or larger in diameter was 3-5 times higher than that 63μm in diameter, indicating a threshold level of the activity appeared between 63 and 200μm in diameter. EC50 values to afratoxin B1 for micro-tissues 200μm in diameter or larger is one order of magnitude lower than that 63μm in diameter and were nearly equal to that for a conventional 96-well plate(6. 4mm in diameter). On the other hand, a well-of-the-well system based culture plate with an oxygen sensing photoluminescence probe was also developed for the determination of a cellular respiratory activity. Thus, the present methodology allows for the determination of the minimum required size of the three-dimensional micro-tissue consisting of organ-derived cells for in vitro toxicity analyses.
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