Analysis of genome-wide DNA methylation profiles of the brain in mice exposed to diesel exhaust during fetal period.

• Project/Area Number: 22710067
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Risk sciences of radiation/Chemicals
• Research Institution: Tokyo University of Science
• Principal Investigator: TACHIBANA Ken 東京理科大学, 薬学部, 助教 (10400540)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2010: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: トキシコロジー / DNAメチル化 / 環境 / 衛生 / エピジェネティクス / ディーゼル排ガス

Research Abstract

Diesel exhaust(DE) was exposed to pregnant mice and genomic DNA was obtained from offspring. Subsequently, genome-wide DNA methylation analysis was performed. The results indicated that prenatal DE exposure disrupts the construction of DNA methylation pattern in the brain of offspring. It is also suggested that disrupted DNA methylation pattern is sustained during growth. Our findings provide the important information to clarify the effects and mechanisms of functional impairment of the brain induced by prenatal DE exposure.

Research Products

• [Journal Article] MEK-ERK signaling dictates DNA-repair gene MGMT expression and temozolomide resistance of stem-like glioblastoma cells via the MDM2-p53 axis (2011)
  • Author(s): Sato A, Sunayama J, Matsuda K, Seino S, Suzuki K, Watanabe E, Tachibana K, Tomiyama A, Kayama T, Kitanaka C.
  • Journal Title: Stem Cells Volume: Vol.29 Pages: 1942-1951
  • Peer Reviewed
• [Journal Article] FoxO3a functions as a key integrator of cellular signals that control glioblastoma stem-like cell differentiation and tumorigenicity (2011)
  • Author(s): Sunayama J, Sato A, Matsuda K, Tachibana K, Watanabe E, Seino S, Suzuki K, Narita Y, Shibui S, Sakurada K, Kayama T, Tomiyama A, Kitanaka C.
  • Journal Title: Stem Cells Volume: Vol.29 Pages: 1327-1337
  • Peer Reviewed
• [Journal Article] DNA配列に無関係な遺伝子発現調節 (2011)
  • Author(s): 立花研
  • Journal Title: 科学フォーラム Volume: 第28巻第1号 Pages: 42-46
• [Journal Article] MEK-ERK signaling dictates DNA-repair gene MGMT expression and temozolomide resistance of stem-like glioblastoma cells via the MDM2-p53 axis (2011)
  • Author(s): Sato A
  • Journal Title: Stem Cells Volume: 29 Issue: 12 Pages: 1942-1951
  • DOI: 10.1002/stem.753
  • Peer Reviewed
• [Journal Article] Fox03a functions as a key integrator of cellular signals that control glioblastoma stem-like cell differentiation and tumorigenicity (2011)
  • Author(s): Sunayama J, Sato A, Matsuda K, Tachibana K, Watanabe E, Seino S, Suzuki K, Narita Y, Shibui S, Sakurada K, Kayama T, Tomiyama A, Kitanaka C
  • Journal Title: Stem Cells Volume: 29 Issue: 9 Pages: 1327-1337
  • DOI: 10.1002/stem.696
  • Peer Reviewed
• [Presentation] ディーゼル排ガス胎仔期曝露によるDNAメチル化異常の網羅的解析 (2012)
  • Author(s): 立花研、高柳皓平、秋本純芽、新海雄介、武田健
  • Organizer: 日本薬学会第132年会
  • Place of Presentation: 札幌(北海道)
  • Year and Date: 2012-03-31
• [Presentation] 環境化学物質により次世代に生じる健康影響 (2012)
  • Author(s): 立花研
  • Organizer: 日本幼少児健康教育学会第30回記念大会
  • Place of Presentation: 野田(千葉)
  • Year and Date: 2012-02-19
• [Presentation] 胎仔期にディーゼル排ガスに曝露されたマウスにおけるDNAメチル化状態の網羅的解析 (2011)
  • Author(s): 高柳皓平、立花研、秋本純芽、新海雄介、武田健
  • Organizer: 日本薬学会第131年会
  • Place of Presentation: 静岡(静岡)
  • Year and Date: 2011-03-30
• [Presentation] 胎仔期にディーゼル排ガスに曝露されたマウスにおけるDNAメチル化状態の網羅的解析 (2011)
  • Author(s): 高柳皓平、立花研、秋本純芽、新海雄介、武田健
  • Organizer: 日本薬学会第131年会
  • Place of Presentation: 静岡
  • Year and Date: 2011-03-30