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Functional analyses of an anammox protein and development of the monitoring method for anammox reaction by using its gene.

Research Project

Project/Area Number 22710078
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Environmental technology/Environmental materials
Research InstitutionSojo University (2011)
Kumamoto University (2010)

Principal Investigator

HIRA Daisuke  崇城大学, 生物生命学部, 助教 (00569890)

Project Period (FY) 2010 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2011: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2010: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Keywordsanammox / ヘム蛋白質 / 嫌気性アンモニア酸化 / 脱窒 / 窒素除去 / Anammox
Research Abstract

Anaerobic ammonium oxidation(anammox) is a novel and promising process for the removal of inorganic nitrogen from wastewater treatment. In this study, I have characterized a heterodimeric heme-protein with a low molecular weight being specific for anammox bacteria to reveal the reaction mechanism of anammox. Biochemical analyses revealed that the heterodimeric heme-protein was involved in the hydrazine synthesis which is a key reaction in anammox process.

Report

(3 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report
  • Research Products

    (17 results)

All 2012 2011 2010

All Journal Article (10 results) (of which Peer Reviewed: 10 results) Presentation (7 results)

  • [Journal Article] Anammox organism KSU-1 expresses a NirK-type copper-containing nitrite reductase instead of a NirS-type with cytochrome cd_12012

    • Author(s)
      Hira D, Toh H, Migita CT, Okubo H, Nishiyama T, Hattori M, Furukawa K, Fujii T
    • Journal Title

      FEBS Letters

      Volume: 586 Pages: 1658-1663

    • URL

      http://dx.doi.org/10.1016/j.febslet.2012.04.041

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Factors affecting the treatment of reject water by the anammox process2011

    • Author(s)
      Li Z, Ma Y, Hira D, Fujii T, Furukawa K
    • Journal Title

      Bioresour. Technol

      Volume: 102 Pages: 5702-5708

    • URL

      http://dx.doi.org/10.1016/j.biortech.2011.03.001

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Reject water treatment by improvement of whole cell anammox entrapment using polyvinyl alcohol/alginate gel2011

    • Author(s)
      Quan LM, Khanh do P, Hira D, Fujii T, Furukawa K
    • Journal Title

      Biodegradation

      Volume: 22 Pages: 1155-1167

    • NAID

      10027707853

    • URL

      http://dx.doi.org/10.1007/s10532-011-9471-3

    • Related Report
      2011 Annual Research Report 2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Effect of temperature on low-strength wastewater treatment by UASB reactor using poly(vinyl alcohol)-gel carrier2011

    • Author(s)
      Khanh D, Quan L, Zhang, Hira D, Furukawa K
    • Journal Title

      Bioresour. Technol

      Volume: 102 Pages: 11147-11154

    • URL

      http://dx.doi.org/10.1016/j.biortech.2011.09.108

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Nitrogen removal performance of a hybrid anammox reactor2011

    • Author(s)
      Ma Y, Hira D, Li Z, Chen C, Furukawa K
    • Journal Title

      Bioresour. Technol

      Volume: 102 Pages: 6650-6656

    • URL

      http://dx.doi.org/10.1016/j.biortech.2011.03.081

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Factors affecting the treatment of reject water by the anammox process2011

    • Author(s)
      Li Z, Ma Y, Hira D, Fujii T, Furukawa K
    • Journal Title

      Bioresour.Technol.

      Volume: 102 Issue: 10 Pages: 5702-5708

    • DOI

      10.1016/j.biortech.2011.03.001

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Effect of temperature on low-strength wastewater treatment by UASB reactor using poly (vinyl alcohol)-gel carrier2011

    • Author(s)
      Khanh D, Quan L, Zhang, Hira D, Furukawa K
    • Journal Title

      Bioresour.Technol.

      Volume: 102 Issue: 24 Pages: 11147-11154

    • DOI

      10.1016/j.biortech.2011.09.108

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A heterodimeric cytochrome c complex with a very low redox potential from an anaerobic ammonium-oxidizing enrichment culture2010

    • Author(s)
      Ukita S, Fujii T, Hira D, Nishiyama T, Kawase T, Migita CT, Furukawa K
    • Journal Title

      FEMS Microbiol. Lett

      Volume: 313 Issue: 1 Pages: 61-67

    • DOI

      10.1111/j.1574-6968.2010.02122.x

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] A heterodimeric c-type cytochrome c complex with very low redox poten tial from an anaerobic ammoniumoxidizing enrichment culture2010

    • Author(s)
      浮田早貴、藤井隆夫、平大輔, 他
    • Journal Title

      FEMS Microbiol.Lett

      Volume: Vol.313 Pages: 61-67

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Nitrogen removal performance of a hybrid anammox reactor2010

    • Author(s)
      馬永光、平大輔, 他
    • Journal Title

      Bioresource Technology

      Volume: (印刷中)

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Presentation] 嫌気性アンモニア酸化(anammox)菌由来NaxLS複合体の結晶構造2012

    • Author(s)
      平大輔、中村照也、山縣ゆり子、古川憲治、藤井隆夫
    • Organizer
      日本農芸化学会大会2012年度大会
    • Place of Presentation
      京都女子大学
    • Year and Date
      2012-03-24
    • Related Report
      2011 Annual Research Report 2011 Final Research Report
  • [Presentation] 嫌気性アンモニア酸化細菌のヘテロ2量体シトクロムcの構造と電子伝達系における機能2011

    • Author(s)
      平大輔、中村照也、山縣ゆり子、古川憲治、藤井隆夫
    • Organizer
      第84回日本生化学会大会
    • Place of Presentation
      国立京都国際会館
    • Year and Date
      2011-09-22
    • Related Report
      2011 Annual Research Report 2011 Final Research Report
  • [Presentation] Structural Analyses of a Heterodimeric Cytochrome c Complex from an Anammox Bactrium Strain KSU-12011

    • Author(s)
      Hira D, Nakamura T, Yamagata Y, Fujii T, Furukawa K
    • Organizer
      First International Anammox Symposium
    • Place of Presentation
      熊本大学
    • Year and Date
      2011-05-20
    • Related Report
      2011 Annual Research Report 2011 Final Research Report
  • [Presentation] Anammox菌の生産するヘテロ2量体c型ヘムタンパク質の構造と機能2011

    • Author(s)
      平大輔、中村照也、山縣ゆり子、古川憲治、藤井隆夫
    • Organizer
      日本農芸化学会2011年度大会
    • Place of Presentation
      京都女子大学
    • Year and Date
      2011-03-26
    • Related Report
      2011 Final Research Report
  • [Presentation] Anammox菌の生産するヘテロ2量体c型ヘムタンパク質の構造と機能2011

    • Author(s)
      平大輔, 他
    • Organizer
      日本農芸化学会2011年度大会
    • Place of Presentation
      京都女子大学(京都府)
    • Year and Date
      2011-03-26
    • Related Report
      2010 Annual Research Report
  • [Presentation] Anammox汚泥からのヘテロ2量体シトクロムc(NaxLS)遺伝子の検出とその一次構造の特徴2010

    • Author(s)
      藤井隆夫、平大輔、古川憲治
    • Organizer
      第62回日本生物工学会大会
    • Place of Presentation
      ワールドコンベンションセンターサミット(宮崎県)
    • Year and Date
      2010-10-28
    • Related Report
      2011 Final Research Report 2010 Annual Research Report
  • [Presentation] Anammox菌のヘテロ2量体シトクロムc(NaxLS)のX線結晶構造解析2010

    • Author(s)
      平大輔、中村照也、山縣ゆり子、古川憲治、藤井隆夫
    • Organizer
      第62回日本生物工学会大会
    • Place of Presentation
      ワールドコンベンションセンターサミット(宮崎県)
    • Year and Date
      2010-10-28
    • Related Report
      2011 Final Research Report 2010 Annual Research Report

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Published: 2010-08-23   Modified: 2016-04-21  

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