Design and Preparation of a Nanodevice Containing Gold Nanoparticles for Delivery and Imaging of Nucleic Acids
| Project/Area Number |
22710114
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Nanomaterials/Nanobioscience
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| Research Institution | University of Tsukuba |
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Principal Investigator |
OISHI Motoi 筑波大学, 数理物質系, 講師 (90419242)
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| Project Period (FY) |
2010 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2010: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
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| Keywords | 金ナノ粒子 / ナノデバイス / ナノゲル粒子 / siRNA / 送達 / 可視化 / ナノゲル粒 |
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| Research Abstract |
A PEGylated nanogel containing gold nanoparticles and siRNAs (nanodevice) was designed and prepared for delivery of nucleic acids and imaging of cancer. The nanodevice shows efficient release of siRNAs in response to intracellular environments, because the thiol-Au interaction caused an exchange reaction between siRNA-SH/Au and thiol-containing glutathione molecules, allowing the efficient RNAi activity in cancer cells. Additionally, the nanodevice also serves as an X-ray CT contrast agent due to the large payload of gold nanoparticles in the core of nanogel. The PEGylated nanogel described here is suitable as a smart nanodevice for delivery of nucleic acids and imaging of cancer.
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Report
(3 results)
Research Products
(11 results)
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[Journal Article] Systemic delivery of siRNA to tumors using a lipid nanoparticle containing a tumor-specific cleavable PEG-lipid2011
Author(s)
Hiroto Hatakeyama, Hidetaka Akita, Erika Itoh, Yasuhiro Hayashi, Motoi Oishi, Yukio Nagasaki, R. Danev, K. Nagayama, N. Kaji, H. Kikuchi, Y. Baba, Harashima H
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Journal Title
Biomaterials
Volume: 32(18)
Issue: 18
Pages: 4-4
DOI
Related Report
Peer Reviewed
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