Scafflods created with electrospun nanofibers to maintain pluripotent stem cell culture

• Project/Area Number: 22710116
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Nanomaterials/Nanobioscience
• Research Institution: Kyoto University
• Principal Investigator: LIU Li 京都大学, 物質-細胞統合システム拠点, 特定研究員 (50380093)
• Co-Investigator(Renkei-kenkyūsha): CHEN Yong 京都大学, 物質-細胞統合システム拠点, 教授 (70512458)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 分子マニビュレーション / ナノファイバー / 幹細胞 / 分化・未分化 / 細胞パターン / 分化・末分化

Research Abstract

Embryonic stem cells(ESCs) are useful resources for drug discovery, developmental biology and disease studies. Cellular microenvironmental cues play critical roles in regulating ESC functions, but it is challenging to control them with synthetic components. Nanofibers hold a potential to create artificial cellular cues for controlling cell adhesion and cell-cell interactions. In this project, I focus to develop a new technology for maintaining pluripotent stem cell culture by electrospun nanofibers. The last two years, I provide a chemical defined scaffold created with electrospun nanofibers made from polymethylglutarimide(PMGI), which is a synthetic thermoplastic polymer stable under culture conditions. mESCs on nanofibers showed a growth rate comparable to those cultured conventionally and they retained their pluripotency. In addition, integrated with micro/nano-engineering technology, I successfully established an approach for patterning pluripotent stem cells.

Research Products

• [Journal Article] Integrated and diffusion-based micro-injectors for open access cell assays (2011)
  • Author(s): X.Li, L.Liu, L.Wang, K.Kamei, Q.Yuan, F.Zhang, J.Shi, A.Kusumi, M.Xie, Z.Zhao, Y.Chen
  • Journal Title: Lab Chip Volume: 11 Issue: 15 Pages: 2612-2617
  • DOI: 10.1039/c1lc20258h
  • Peer Reviewed
• [Journal Article] Fabrication of polymer fiber scaffolds by centrifugal spinning for cell culture studies (2011)
  • Author(s): Li Wang, Jian Shi, Li Liu, Emilie Secret, Yong Chen
  • Journal Title: Microelectron. Eng. Volume: 88 Issue: 8 Pages: 1718-1721
  • DOI: 10.1016/j.mee.2010.12.054
  • Peer Reviewed
• [Journal Article] Investigation of cell culture in microfluidic devices with differet bi-layer substrate (2011)
  • Author(s): Jian Shi, Li Liu, Yong Chen
  • Journal Title: Microelectron. Eng. Volume: 88 Issue: 8 Pages: 1693-1697
  • DOI: 10.1016/j.mee.2011.01.047
  • Peer Reviewed
• [Journal Article] Differentiating stem cells on patterned substrates for neural network formation (2011)
  • Author(s): Chunxiong Luo, Li Liu, Xiaofang Ni, Li Wang, Shi-ichiro Nomura, Quyuan Qi, Yong Chen
  • Journal Title: Microelectron. Eng. Volume: 88 Issue: 8 Pages: 1707-1710
  • DOI: 10.1016/j.mee.2010.12.062
  • Peer Reviewed
• [Journal Article] Differentiating stem cells on patterned substrates for neural net-work formation (2011)
  • Author(s): Chunxiong Luo, Li Liu, Xiaofang Ni, Li Wang, Shi-ichiro Nomura, Ouyuang Qi, Yong Chen (contributed equally)
  • Journal Title: Microelectronic Engineering Volume: (印刷中)
  • Peer Reviewed
• [Journal Article] Investigation of cell culture in microfluidic devices with differentbi-layer substrate (2011)
  • Author(s): Jian Shi, Li Liu, Yong Chen (contributed equally)
  • Journal Title: Microelectronic Engineering Volume: (印刷中)
  • Peer Reviewed
• [Journal Article] Chemically defined scaffolds created with electrospun synthetic nanofibers to maintain mouse embryonic stem cell culture under feeder-free conditions
  • Author(s): Li Liu, Qinghua Yuan, Jian Shi, Xin Li, Dong Jung, Li Wang, Kaori Yamauchi, Norio Nakatsuji, Ken-ichiro Kamei, Yong. Chen
  • Journal Title: Biotechnol. Lett. Volume: (in press)
• [Presentation] Title : Electrospun nanofibers for creating artificial cellular microenvironments for culturing mouse embryonic stem cells (2011)
  • Author(s): Li Liu, Qinghua Yuan, Jian Shi, Li Wang, Xin Li, Dong Jung, Kaori Yamauchi, Norio Nakatsuji, Ken-ichiro Kamei, Yong Chen
  • Organizer: 2^<nd> Nano Today
  • Place of Presentation: Hawaii
• [Presentation] Electrospun nanofibers for creating artificial cellular microenvironments for culturing mouse embryonic stem cells (2011)
  • Author(s): L.Liu, Q.H.Yuan, J.Shi, L.Wang, X.Li, D.Jung, K.Yamauchi, N.Nakatsuji, K.Kamei, Y.Chen
  • Organizer: 2nd Nano Today
  • Place of Presentation: ハワイ(アメリカ)
• [Presentation] Title : Long-term feeder-free culture of mouse embryonic stem cells on electrospun nanofibers (2010)
  • Author(s): Li Liu, Q. Yuan, K Kamei, Jian Shi, Xin Li, Li Wang, Dong Jung, Kaori Yamauchi, Norio Nakatsuji and Yong Chen
  • Organizer: 8^<th> iCeMS Interventional Symposium, Meso-Control of Functional Architectures
  • Place of Presentation: Kyoto