Development of the on chip microchamber array device for single cell gene analysis
| Project/Area Number |
22710122
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Microdevices/Nanodevices
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| Research Institution | Osaka University |
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Principal Investigator |
SAITO Masato 大阪大学, 大学院・工学研究科, 助教 (80457001)
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| Project Period (FY) |
2010 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | シングルセル / PCR / RT-PCR / マイクロチャンバー / マイクロ・ナノデバイス / マイクロバイオシステム |
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| Research Abstract |
For analyzing a single cell contents or gene information, we have fabricated the microchamber array chip which has microscale reaction fields. One of chip substrate material was silicon and other one was polystylen polymer. After evaluation and optimization of the on-chip PCR conditions, DNA amplification from a single cell in the microchamber, was attempted. A single cells were placed in each microchambers by micromanipulating. As a result, we could successfully observed the fluorescence which was associated with DNA amplification from a single cells.
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Report
(3 results)
Research Products
(26 results)
