| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Research Abstract |
For analyzing a single cell contents or gene information, we have fabricated the microchamber array chip which has microscale reaction fields. One of chip substrate material was silicon and other one was polystylen polymer. After evaluation and optimization of the on-chip PCR conditions, DNA amplification from a single cell in the microchamber, was attempted. A single cells were placed in each microchambers by micromanipulating. As a result, we could successfully observed the fluorescence which was associated with DNA amplification from a single cells.
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