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Development of a cell microarray chip device for diagnosis of infectious disease

Research Project

Project/Area Number 22710124
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Microdevices/Nanodevices
Research InstitutionNational Institute of Advanced Industrial Science and Technology

Principal Investigator

YAMAMURA Shohei  独立行政法人産業技術総合研究所, 健康工学研究部門, 研究員 (50432141)

Project Period (FY) 2010 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2010: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Keywords細胞チップ / マイクロアレイ / バイオチップ / 単一細胞解析 / ハイスループットスクリーニング / 感染症 / マラリア
Research Abstract

Malaria is a vector-borne infectious disease caused by protozoan parasites. There are approximately 500 million cases of malaria, killing over two million people each year. The most standard detection method ; Giemsa-stained blood smears to detect malaria parasite in erythrocytes is time-consuming and need to technical handling. Although the detection methods based on immunochromatography and PCR were developed, they also have some problems in sensitivity and rapidity. In this study, we have developed a novel cell microarray system to detect malaria-infected erythrocytes with the high-sensitivity(<0.0001%) in a short time.

Report

(3 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report
  • Research Products

    (23 results)

All 2012 2011 2010 Other

All Journal Article (6 results) (of which Peer Reviewed: 6 results) Presentation (16 results) Remarks (1 results)

  • [Journal Article] Accurate detection of carcinoma cells by use of a cell microarray chip2012

    • Author(s)
      Yamamura S, Yatsushiro S, Yamaguchi Y., Abe K., Shinohara Y., Tamiya E., Baba Y., Kataoka M.
    • Journal Title

      PloS One

      Volume: 7 Issue: 3 Pages: e32370-e32370

    • DOI

      10.1371/journal.pone.0032370

    • Related Report
      2011 Annual Research Report 2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Development of a cell microarray chip for detection of circulating tumor cells2012

    • Author(s)
      Yamamura S, Yatsushiro S, Abe K, Baba Y, Kataoka M.
    • Journal Title

      J. Phys. Conf. Ser.

      Volume: 352 Pages: 12041-12041

    • DOI

      10.1088/1742-6596/352/1/012041

    • Related Report
      2011 Annual Research Report 2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Ribonuclease protection assay on microchip electrophoresis2011

    • Author(s)
      Yamaguchi Y, Yatsushiro S, Yamamura S, Abe H., Abe K., Watanabe M., Kajimoto K., Shinohara Y., Baba Y., Kataoka M.
    • Journal Title

      Analyst

      Volume: 136 Issue: 11 Pages: 2247-2251

    • DOI

      10.1039/c0an01044h

    • Related Report
      2011 Annual Research Report 2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Rapid and Highly Sensitive Detection of Malaria-Infected Erythrocytes Using a Cell Microarray Chip2010

    • Author(s)
      Yatsushiro S, Yamamura S, Yamaguchi Y, Shinohara Y, Tamiya E, Horii T, Baba Y, Kataoka M
    • Journal Title

      PloS One

      Volume: 5 Issue: 10 Pages: e13179-e13179

    • DOI

      10.1371/journal.pone.0013179

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Development of Cell Chip System for Cytological Analysis and Diagnosis2010

    • Author(s)
      山村昌平,八代聖基,山口裕加,民谷栄一,片岡正俊
    • Journal Title

      IEEJ Transactions on Electronics, Information and Systems

      Volume: 130 Issue: 10 Pages: 1795-1799

    • DOI

      10.1541/ieejeiss.130.1795

    • NAID

      130000415114

    • ISSN
      0385-4221, 1348-8155
    • Related Report
      2011 Final Research Report 2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Rapid and Highly Sensitive Detection of Malaria-Infected Erythrocytes Using a Cell Microarray Chip2010

    • Author(s)
      Yatsushiro S., Yamamura S., Yamaguchi Y., Shinohara Y., Tamiya E., Horii T., Baba Y., Kataoka M.
    • Journal Title

      PloS One

      Volume: 5

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Presentation] 生活習慣病の診断用マイクロチップ、ヘルスケア・イノベーションフォーラム(HCIF)2011

    • Author(s)
      山村昌平、阿部佳織、八代聖基、片岡正俊
    • Organizer
      第11回事例研究部会(招待講演)
    • Place of Presentation
      香川県
    • Year and Date
      2011-11-25
    • Related Report
      2011 Final Research Report
  • [Presentation] 生活習慣病の診断用マイクロチップ2011

    • Author(s)
      山村昌平、阿部佳織、八代聖基、片岡正俊
    • Organizer
      ヘルスケア・イノベーションフォーラム(HCIF)第11回事例研究部会
    • Place of Presentation
      香川県、高松合同庁舎(招待講演)
    • Year and Date
      2011-11-25
    • Related Report
      2011 Annual Research Report
  • [Presentation] Development of a cell microarray chip for detection of circulating tumor cells2011

    • Author(s)
      Yamamura S, Yatsushiro S, Abe K, Baba Y, Kataoka M
    • Organizer
      The Asia-Pacific Interdisciplinary Research Conference 2011(AP-IRC 2011)
    • Place of Presentation
      愛知県
    • Year and Date
      2011-11-18
    • Related Report
      2011 Final Research Report
  • [Presentation] Development of a cell microarray chip for detection of circulating tumor cells2011

    • Author(s)
      Yamamura S, Yatsushiro S, Abe K, Baba Y, Kataoka M
    • Organizer
      The Asia-Pacific Interdisciplinary Research Conference 2011 (AP-IRC 2011)
    • Place of Presentation
      愛知県、豊橋技術科学大学
    • Year and Date
      2011-11-18
    • Related Report
      2011 Annual Research Report
  • [Presentation] Quantitative analysis of serum interleukin-6 by immunoassay on microchip2011

    • Author(s)
      Abe K, Yatsushiro S, Yamamura S, Tanaka M, Ooie T, Baba Y, Kataoka M
    • Organizer
      The Asia-Pacific Interdisciplinary Research Conference 2011(AP-IRC 2011)
    • Place of Presentation
      愛知県
    • Year and Date
      2011-11-17
    • Related Report
      2011 Final Research Report
  • [Presentation] Quantitative analysis of serum interleukin-6 by immunoassay on microchip2011

    • Author(s)
      Abe K, Yatsushiro S, Yamamura S, Tanaka M, Ooie T, Baba Y
    • Organizer
      The Asia-Pacific Interdisciplinary Research Conference 2011 (AP-IRC 2011)
    • Place of Presentation
      愛知県、豊橋技術科学大学
    • Year and Date
      2011-11-17
    • Related Report
      2011 Annual Research Report
  • [Presentation] 各種疾患診断を目指した細胞チップの開発2011

    • Author(s)
      山村昌平
    • Organizer
      第3回若手異分野連携体セミナー(招待講演)
    • Place of Presentation
      岡山県
    • Year and Date
      2011-07-15
    • Related Report
      2011 Final Research Report
  • [Presentation] 各種疾患診断を目指した細胞チップの開発2011

    • Author(s)
      山村昌平
    • Organizer
      第3回若手異分野連携体セミナー
    • Place of Presentation
      岡山県、岡山大学(招待講演)
    • Year and Date
      2011-07-15
    • Related Report
      2011 Annual Research Report
  • [Presentation] 細胞チップを用いた感染症迅速診断法の開発2011

    • Author(s)
      山村昌平、八代聖基、山口裕加、片岡正俊
    • Organizer
      日本化学会第91春季年会
    • Place of Presentation
      神奈川県
    • Year and Date
      2011-03-28
    • Related Report
      2011 Final Research Report
  • [Presentation] 細胞チップを用いた感染症迅速診断法の開発2011

    • Author(s)
      山村昌平、八代聖基、山口裕加、片岡正俊
    • Organizer
      日本化学会第91春季年会
    • Place of Presentation
      神奈川
    • Year and Date
      2011-03-28
    • Related Report
      2010 Annual Research Report
  • [Presentation] Cell microarray chip for rapid and highly sensitive detection of malaria-infected erythrocytes2011

    • Author(s)
      Yamamura S, Yatsushiro S, Yuka Yamaguchi, Kataoka M
    • Organizer
      The 5^<nd> International Workshop on Approaches to Single-cell Analysis
    • Place of Presentation
      東京都
    • Year and Date
      2011-03-04
    • Related Report
      2011 Final Research Report
  • [Presentation] Cell microarray chip for rapid and highly sensitive detection of malaria-infected erythrocytes2011

    • Author(s)
      Shohei Yamamura, Shouki Yatsushiro, Yuka Yamaguchi, Masatoshi Kataoka
    • Organizer
      The 5^<nd> International Workshop on Approaches to Single-cell Analysis
    • Place of Presentation
      東京
    • Year and Date
      2011-03-04
    • Related Report
      2010 Annual Research Report
  • [Presentation] Rapid and highly sensitive detection system for malaria parasite with a cell microarray chip2010

    • Author(s)
      Yatsushiro S, Yamamura S, Kataoka M
    • Organizer
      The 3rd Stakeholders Meeting of the African Network for Drugs and Diagnostics Innovation(ANDI)
    • Place of Presentation
      Kenya(Nairobi)
    • Year and Date
      2010-10-11
    • Related Report
      2011 Final Research Report
  • [Presentation] Rapid and highly sensitive detection system for malaria parasite with a cell microarray chip2010

    • Author(s)
      Shouki Yatsushiro, Shohei Yamamura, Masatoshi Kataoka
    • Organizer
      The 3rd Stakeholders Meeting of the African Network for Drugs and Diagnostics Innovation (ANDI)
    • Place of Presentation
      Kenya(Nairobi)
    • Year and Date
      2010-10-11
    • Related Report
      2010 Annual Research Report
  • [Presentation] 細胞チップを用いたマラリア原虫迅速検出手法の構築2010

    • Author(s)
      八代聖基、山村昌平、山口裕加、堀井俊宏、片岡正俊
    • Organizer
      第51回日本生化学会中国四国支部会
    • Place of Presentation
      山口県
    • Year and Date
      2010-05-14
    • Related Report
      2011 Final Research Report
  • [Presentation] 細胞チップを用いたマラリア原虫迅速検出手法の構築2010

    • Author(s)
      八代聖基、山村昌平、山口裕加、堀井俊宏、片岡正俊
    • Organizer
      第51回日本生化学会中国四国支部会
    • Place of Presentation
      山口
    • Year and Date
      2010-05-14
    • Related Report
      2010 Annual Research Report
  • [Remarks]

    • URL

      http://unit.aist.go.jp/hri/group/barg/

    • Related Report
      2011 Final Research Report

URL: 

Published: 2010-08-23   Modified: 2016-04-21  

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