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Development of label-free biochip for early diagnosis of Alzheimer's disease using artificial nano-receptor particles

Research Project

Project/Area Number 22710125
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Microdevices/Nanodevices
Research InstitutionNational Institute of Advanced Industrial Science and Technology

Principal Investigator

FUCHIWAKI Yusuke  独立行政法人産業技術総合研究所, 健康工学研究部門, 研究員 (80468884)

Project Period (FY) 2010 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2011: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2010: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Keywordsバイオチップ / 分子インプリント / LSPR / 金ナノ / インクジェット / MIP / ナノ材料 / マイクロナノデバイス / チップ / 非標識 / アルツハイマー
Research Abstract

We studied to develop a novel label-free biochip device for early diagnosis of Alzheimer's disease using molecularly imprinted-based artificial receptors. In order to improve the nonspecific adsorption on surface of nano-artificial receptors, the newly functional polymer, which includes both phophorylcholine and amino groups, was synthesized and accomplished to increase signal to noise ratio. Ink-jet printing technology has been included for the accurate preparation of bio-recognition interface, and got in about three times lower variations from calibration curves as compared to previous studies.

Report

(3 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report
  • Research Products

    (40 results)

All 2012 2011 2010

All Journal Article (20 results) (of which Peer Reviewed: 14 results) Presentation (19 results) Book (1 results)

  • [Journal Article] Immunosensor Accuracy is Improved by Coating Beads with Poly-2-[3-(Methacryloylamino) Propyla mmonio] Ethyl 3-Aminopropyl Phosphate2012

    • Author(s)
      Yusuke Fuchiwaki、Mikito Yasuzawa
    • Journal Title

      Anal. Lett.

      Volume: 45巻 Issue: 2-3 Pages: 262-271

    • DOI

      10.1080/00032719.2011.633180

    • Related Report
      2011 Annual Research Report 2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Characteristics of Molecularly Imprinted Polymer(MIP) Thin Layer for Bisphenol A and Response of the MIP-Modified Sensor2012

    • Author(s)
      Izumi Kubo、Nobuyuki Yokota、Yusuke Fuchiwaki、Yuko Nakane
    • Journal Title

      ISRN Materials Science

      Volume: Vol.2012 Pages: 6-6

    • DOI

      10.5402/2012/861643

    • Related Report
      2011 Annual Research Report 2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Fabrication of the printed microchip toward point-of-care testing2012

    • Author(s)
      Yusuke Fuchiwaki、Yuichi Yabe、Yoshinori Adachi、Masato Tanaka、Kaori Abe、Tomonori Nakahara、Yuji Yamachoshi、Masatoshi Kataoka、Toshihiko Ooie
    • Journal Title

      Chemical Sensors

      Volume: 28A巻 Pages: 58-60

    • NAID

      40019318615

    • URL

      http://chemsens.electrochem.jp/journal/2012/2012a-abt.htm

    • Related Report
      2011 Final Research Report
  • [Journal Article] Development of ultra rapid flow-through PCR microfluidics devices using vapor pressure2012

    • Author(s)
      Yusuke Fuchiwaki, Hidenori Nagai, Masato Saito, Shin-ichi Wakida, Masatoshi Kataoka, Toshihiko Ooie, Eiichi Tamiya
    • Journal Title

      Chemical Sensors

      Volume: 28 Supplement A Pages: 64-66

    • NAID

      40019318629

    • Related Report
      2011 Annual Research Report
  • [Journal Article] Fabrication of the printed microchip toward point-of-care testing2012

    • Author(s)
      Yusuke Fuchiwaki, Yuichi Yabe, Yoshinori Adachi, Masato Tanaka, Kaori Abe, Tomonori Nakahara, Yuji Yamachoshi, Masatoshi Kataoka, Toshihiko Ooie
    • Journal Title

      Chemical Sensors

      Volume: 28 Supplement A Pages: 58-60

    • NAID

      40019318615

    • Related Report
      2011 Annual Research Report
  • [Journal Article] Improvement of accuracy in flow immunosensor system by introduction of poly-2-[3-(methacryloylamino) propyla mmonio] ethyl 3-aminopropyl phosphate2011

    • Author(s)
      Yusuke Fuchiwaki、Mikito Yasuzawa、Norimichi Futagami、Kotaro Rikitake
    • Journal Title

      Journal of Sensors

      Volume: V0l. 2011 Pages: 10-10

    • DOI

      10.1155/2011/507047

    • Related Report
      2011 Annual Research Report 2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Flow immunosensing system utilizing antibodies immobilized on beads coated with poly-2-[3-(methacryloylamino) propyla mmonio] ethyl 3-aminopropyl phosphate2011

    • Author(s)
      Yusuke Fuchiwaki、Mikito Yasuzawa
    • Journal Title

      Sensors and Actuators A

      Volume: 170巻 Issue: 1-2 Pages: 100-105

    • DOI

      10.1016/j.sna.2011.05.025

    • Related Report
      2011 Annual Research Report 2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Selectivity and Regeneratability Evaluation of Antigen Analogous-Modified Surface Film for Polychlorinated Dibenzodioxin Immunosensor Using Surface Plasmon Resonance Measurement2011

    • Author(s)
      安澤幹人、二神規道、渕脇雄介、薮谷智樹、本仲純子
    • Journal Title

      BUNSEKI KAGAKU

      Volume: 60 Issue: 1 Pages: 81-86

    • DOI

      10.2116/bunsekikagaku.60.81

    • NAID

      10027410005

    • ISSN
      0525-1931
    • Related Report
      2011 Final Research Report 2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] bisphenol Aインプリントポリマー薄膜の特性とセンサ応答2011

    • Author(s)
      Izumi Kubo、Nobuyuki Yokota、Yuko Nakane、Tomoyuki Kanematsu、Yusuke Fuchiwaki
    • Journal Title

      Chemical Sensors

      Volume: 27A巻 Pages: 19-21

    • URL

      http://chemsens.electrochem.jp/journal/2011/2011a-abt.htm

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] The Establishment of Bisphenol A Sensing System Utilizing Molecularly Imprinted Receptor and Electrochemical Determination2011

    • Author(s)
      I. KUBO, N. YOKOTA, Y. NAKANE, Y. FUCHIWAKI
    • Journal Title

      International Journal of Electrochemistry

      Volume: Vol.2011 Pages: 4-4

    • DOI

      10.4061/2011/534936

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] PORTABLE DNA DETECTION SYSTEM BASED ON ULTRAFAST SEGMENT-FLOW AND FLUORESCENCE DETECTION2011

    • Author(s)
      Hidenori Nagai, Yusuke Fuchiwaki, Keiichiro Yamanaka, Masato Saito, Eiichi Tamiya
    • Journal Title

      The Proceedings of Micro Total Analysis Systems 2011

      Volume: 2011 CBMS Pages: 503-505

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Ultra-rapid flow-through polymerase chain reaction microfluidics using vapor pressure2011

    • Author(s)
      Yusuke Fuchiwaki, Hidenori Nagai, Masato Saito, Eiichi Tamiya
    • Journal Title

      Biosensors and Bioelectronics

      Volume: 27 Issue: 1 Pages: 88-94

    • DOI

      10.1016/j.bios.2011.06.022

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Study of DNA Amplification Efficiency Based on Temperature Analyses of the Moving Fluid in a Liquid-Plug Flow PCR System2011

    • Author(s)
      Yusuke Fuchiwaki, Hidenori Nagai, Masato Saito, Eiichi Tamiya
    • Journal Title

      Bulletin of the Chemical Society of Japan

      Volume: 84 Issue: 10 Pages: 1075-1081

    • DOI

      10.1246/bcsj.20110130

    • NAID

      130004152815

    • ISSN
      0009-2673, 1348-0634
    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A practical liquid plug flow-through polymerase chain reaction system based on a heat resistant resin chip2011

    • Author(s)
      渕脇雄介、斉藤真人、脇田慎一、民谷栄一、永井秀典
    • Journal Title

      Analytical Sciences

      Volume: 27 Pages: 225-230

    • NAID

      10027905970

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Immunosensor Accuracy is Improved by Coating Beads with Poly-2-[3-(Methacryloylamino)Propylammonio]Ethyl 3-Aminopropyl Phosphate2011

    • Author(s)
      渕脇雄介、安澤幹人
    • Journal Title

      Analytical Letters

      Volume: (Web 掲載確定)

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] bisphenol Aインプリントポリマー薄膜の特性とセンサ応答2011

    • Author(s)
      久保いづみ、横田宣之、中根優子、金松知幸、渕脇雄介
    • Journal Title

      Chemical Sensors

      Volume: 27 Pages: 19-21

    • Related Report
      2010 Annual Research Report
  • [Journal Article] Development of ultra rapid flow-through PCR chip2010

    • Author(s)
      渕脇雄介、斉藤真人、民谷栄一、脇田慎一、永井秀典
    • Journal Title

      Chemical Sensors

      Volume: 26 Pages: 181-183

    • NAID

      40017082342

    • Related Report
      2010 Annual Research Report
  • [Journal Article] 超高速フロースルーPCR法を組み合わせた電気化学的遺伝子検知チップの開発2010

    • Author(s)
      渕脇雄介、斉藤真人、民谷栄一、永井秀典
    • Journal Title

      電気学会論文誌E(センサ・マイクロマシン部門誌)

      Volume: BMS-10 Pages: 37-40

    • NAID

      10026492031

    • Related Report
      2010 Annual Research Report
  • [Journal Article] ULTRA-FAST AND HIGHLY-EFFICIENT FLOW-THROUGH PCR MICROFLUIDICS USING VAPOR PRESSURE AND ITS APPLICATION TO RAPID FIELC DETECTION2010

    • Author(s)
      渕脇雄介、斉藤真人、脇田慎一、民谷栄一、永井秀典
    • Journal Title

      The Proceedings of Micro Total Analysis Systems 2010

      Volume: (CD-ROM)

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The establishment of bisphenol A (BPA) sensing system utilizing molecularly imprinted polymer receptor and electrochemical determination2010

    • Author(s)
      久保いづみ、横田宣之、中根優子、渕脇雄介
    • Journal Title

      International Journal of Electrochemistry

      Volume: (Web)

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Presentation] Point of Care(POC)診断用プリンテッドマイクロチップの構築2012

    • Author(s)
      渕脇雄介、矢部雄一、安達良紀、田中正人、阿部佳織、中原伴徳、山瓶子勇次、片岡正俊、大家利彦
    • Organizer
      第53回化学センサ研究発表会
    • Place of Presentation
      静岡県
    • Year and Date
      2012-03-29
    • Related Report
      2011 Final Research Report
  • [Presentation] Point of Care (POC)診断用プリンテッドマイクロチップの構築2012

    • Author(s)
      渕脇雄介、矢部雄一、安達良紀、田中正人、阿部佳織、中原伴徳、山瓶子勇次、片岡正俊、大家利彦
    • Organizer
      第53回化学センサ研究発表会
    • Place of Presentation
      静岡県、アクトシティ浜松
    • Year and Date
      2012-03-29
    • Related Report
      2011 Annual Research Report
  • [Presentation] 蒸気圧を利用した超高速PCRマイクロ流体デバイスの開発2012

    • Author(s)
      渕脇雄介、永井秀典、斉藤真人、脇田慎一、片岡正俊、大家利彦、民谷栄一
    • Organizer
      第53回化学センサ研究発表会
    • Place of Presentation
      静岡県、アクトシティ浜松
    • Year and Date
      2012-03-29
    • Related Report
      2011 Annual Research Report
  • [Presentation] 超高速PCRマイクロ流体チップの開発とバイオセキュリティへの実用展開2012

    • Author(s)
      渕脇雄介
    • Organizer
      日本化学会第92春季年会(2012)
    • Place of Presentation
      神奈川県、慶応大学
    • Year and Date
      2012-03-26
    • Related Report
      2011 Annual Research Report
  • [Presentation] バイオ関連機能紙の特性と分析・診断チップへの展望2012

    • Author(s)
      渕脇雄介
    • Organizer
      材料フォーラム「ナノバイオデバイス」分科会
    • Place of Presentation
      茨城県、産総研つくばセンター
    • Year and Date
      2012-02-01
    • Related Report
      2011 Annual Research Report
  • [Presentation] 現場型遺伝子検査用超高速PCRデバイスの開発2011

    • Author(s)
      渕脇雄介、永井秀典、斉藤真人、民谷栄一
    • Organizer
      2011年日本化学会西日本大会
    • Place of Presentation
      徳島県、徳島大学
    • Year and Date
      2011-11-12
    • Related Report
      2011 Annual Research Report
  • [Presentation] プリンタブル紙バイオチップを志向したバイオインクジェットシステムの高機能化2011

    • Author(s)
      渕脇雄介、田中正人、内村浩美、片岡正俊、大家利彦
    • Organizer
      第50回機能紙研究発表・講演会
    • Place of Presentation
      高知県、高知県立県民文化ホール
    • Year and Date
      2011-10-27
    • Related Report
      2011 Annual Research Report
  • [Presentation] パルスインジェクター2011

    • Author(s)
      田中正人、出島秀一、中原伴徳、渕脇雄介、大家利彦
    • Organizer
      BioJapan 2011
    • Place of Presentation
      神奈川県
    • Year and Date
      2011-10-05
    • Related Report
      2011 Final Research Report
  • [Presentation] パルスインジェクター2011

    • Author(s)
      田中正人、出島秀一、中原伴徳、渕脇雄介、大家利彦
    • Organizer
      BioJapan 2011
    • Place of Presentation
      神奈川県、パシフィコ横浜
    • Year and Date
      2011-10-05
    • Related Report
      2011 Annual Research Report
  • [Presentation] PORTABLE DNA DETECTION SYSTEM BASED ON ULTRAFAST SEGMENT-FLOW AND FLUORESCENCE DETECTION2011

    • Author(s)
      永井秀典、渕脇雄介、山中啓一郎、斉藤真人、民谷栄一
    • Organizer
      The 15th International Conference on Miniaturized Systems for Chemistry and Life Science
    • Place of Presentation
      米国、シアトル
    • Year and Date
      2011-10-03
    • Related Report
      2011 Annual Research Report
  • [Presentation] Portable DNA Detection System Using Segment-flow PCR2011

    • Author(s)
      永井秀典、渕脇雄介、斉藤真人、民谷栄一
    • Organizer
      JAIMA conference
    • Place of Presentation
      千葉県、幕張メッセ
    • Year and Date
      2011-09-08
    • Related Report
      2011 Annual Research Report
  • [Presentation] セグメントフローPCRを用いた超高速遺伝子検知システムの開発2011

    • Author(s)
      永井秀典、渕脇雄介、斉藤真人、民谷栄一
    • Organizer
      第23回化学とマイクロ・ナノシステム研究会
    • Place of Presentation
      千葉県、千葉大学
    • Year and Date
      2011-06-11
    • Related Report
      2011 Annual Research Report
  • [Presentation] Ultra-fast DNA detection using a PCR microfluidic device2011

    • Author(s)
      永井秀典、渕脇雄介、山中啓一郎、斉藤真人、民谷栄一
    • Organizer
      IUPAC International Congress on Analytical Sciences 2011
    • Place of Presentation
      京都府、国立京都国際会館
    • Year and Date
      2011-05-26
    • Related Report
      2011 Annual Research Report
  • [Presentation] bisphenol Aインプリントポリマー薄膜の特性とセンサ応答2011

    • Author(s)
      久保いづみ、横田宣之、中根優子、金松知幸、渕脇雄介
    • Organizer
      第51回化学センサ研究発表会
    • Place of Presentation
      横浜国立大(神奈川県)
    • Year and Date
      2011-03-29
    • Related Report
      2010 Annual Research Report
  • [Presentation] Ultra rapid flow-through PCR system with disposable electrochemical printed (DEP) chip2010

    • Author(s)
      渕脇雄介、斉藤真人、脇田慎一、民谷栄一、永井秀典
    • Organizer
      PACIFICHEM 2010
    • Place of Presentation
      ハワイ(アメリカ)
    • Year and Date
      2010-12-17
    • Related Report
      2010 Annual Research Report
  • [Presentation] 超高速遺フロースルーPCRチップの開発とオンサイト遺伝子検知への応用2010

    • Author(s)
      渕脇雄介、斉藤真人、脇田慎一、民谷栄一、永井秀典
    • Organizer
      第5回ナノバイオデバイスワークショップ
    • Place of Presentation
      産業技術総合研究所関西センター(大阪府)
    • Year and Date
      2010-12-01
    • Related Report
      2010 Annual Research Report
  • [Presentation] オンサイト遺伝子迅速検知チップの開発2010

    • Author(s)
      渕脇雄介
    • Organizer
      2010産総研オープンラボ
    • Place of Presentation
      産業技術総合研究所つくば中央(茨城県)
    • Year and Date
      2010-10-15
    • Related Report
      2010 Annual Research Report
  • [Presentation] ULTRA-FAST AND HIGHLY-EFFICIENT FLOW-THROUGH PCR MICROFLUIDICS USING VAPOR PRESSURE AND ITS APPLICATION TO RAPID FIELC DETECTION2010

    • Author(s)
      渕脇雄介、斉藤真人、脇田慎一、民谷栄一、永井秀典
    • Organizer
      μ-TAS 2010
    • Place of Presentation
      グローニンゲン(オランダ)
    • Year and Date
      2010-10-05
    • Related Report
      2010 Annual Research Report
  • [Presentation] 超高速フロースルーPCR法を組み合わせた電気化学的遺伝子検知チップの開発2010

    • Author(s)
      渕脇雄介、斉藤真人、民谷栄一、永井秀典
    • Organizer
      2010年度電気学会バイオ・マイクロシステム研究会
    • Place of Presentation
      東京大学(東京都)
    • Year and Date
      2010-06-18
    • Related Report
      2010 Annual Research Report
  • [Book] Biomimetics, Learning from nature2010

    • Author(s)
      渕脇雄介、久保いづみ
    • Publisher
      INTECH
    • Related Report
      2011 Final Research Report

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Published: 2010-08-23   Modified: 2016-04-21  

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