Development of a digital PCR assay using single cell isolationby large arrays of microwells on a lab-on-a-disk

• Project/Area Number: 22710126
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Microdevices/Nanodevices
• Research Institution: National Institute of Advanced Industrial Science and Technology
• Principal Investigator: NAGAI Hidenori 独立行政法人産業技術総合研究所, 健康工学研究部門, 主任研究員 (40357596)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: マイクロバイオシステム / 一細胞アッセイ / 細胞アッセイ / PCR / マイクロ流体 / マイクロデバイス / マイクロアレイ / 遺伝子 / 一細胞 / 単離 / 微小分割

Research Abstract

Aiming at the early detection of various diseases, such as cancer, a centrifugal disk for a single-cell PCR assay was developed. Instead of the large-sized and expensive cell sorter, the cell isolation based on a simple minute division was achieved byusin

Research Products

• [Journal Article] Detection of expressed gene in isolated single cells in microchambers by a novel hot cell-direct RT-PCR method (2012)
  • Author(s): Shunsuke Furutani
  • Journal Title: Analyst Volume: 137 Issue: 13 Pages: 2951-2957
  • DOI: 10.1039/c2an15866c
  • Peer Reviewed
• [Journal Article] Microarray of human P450 with an integrated oxygen sensing film for high-throughput detection ofmetabolic activities (2012)
  • Author(s): Gang Chan、森芳直、森里織、入江隆、永井秀典、後藤達志、達吉郎、今石浩正、森垣憲一
  • Journal Title: Analytical Chemistry Volume: 84 Issue: 12 Pages: 5292-5297
  • DOI: 10.1021/ac300355w
  • Peer Reviewed
• [Journal Article] Compact disk (CD)-shaped device for single cell isolation and PCR of a specific gene in the isolated cell (2010)
  • Author(s): 古谷俊介、永井秀典、高村禅、久保いづみ
  • Journal Title: Analytical and Bioanalytical Chemistry Volume: 398 Issue: 7-8 Pages: 2997-3004
  • DOI: 10.1007/s00216-010-4205-7
  • Peer Reviewed
• [Journal Article] Compact disk (CD)-shaped device for single cell isolation and PCR of aspecific gene in the isolated cell (2010)
  • Author(s): 古谷俊介、永井秀典、高村禅、久保いづみ
  • Journal Title: ANALYTICAL AND BIOANALYTICAL CHEMISTRY Volume: 398 Pages: 2997-3004
  • Peer Reviewed
• [Presentation] Rotatable reagent cartridge for high-performance microvalve system on a centrifugal microfluidicdevice (2013)
  • Author(s): 川井隆之
  • Organizer: Pittcon 2013
  • Place of Presentation: Pennsylvania Convention Center(Philadelphia,USA)
  • Year and Date: 2013-03-17
• [Presentation] Rotatable Reagent Cartridge for High-Performance Microvalve System on a Centrifugal Microfluidic Device (2013)
  • Author(s): Takayuki Kawai
  • Organizer: Pittcon 2013
  • Place of Presentation: Philadelphia
• [Presentation] CD 型細胞単離デバイス上でのHot cell-direct RT-PCR による単一細胞の発現遺伝子検出 (2012)
  • Author(s): 古谷俊介
  • Organizer: 日本化学会第92 春季年会
  • Place of Presentation: 慶應義塾大学(東京都)
  • Year and Date: 2012-03-28
• [Presentation] CD型細胞単離デバイス上でのHot cell-direct RT-PCRによる単一細胞の発現遺伝子検出 (2012)
  • Author(s): 古谷俊介、永井秀典、高村禅、青山由利、久保いづみ
  • Organizer: 日本化学会第92春季年会
  • Place of Presentation: 東京都、慶應義塾大学
  • Year and Date: 2012-03-28
• [Presentation] Cell isolation and celldirect RT-PCR on a compact disk (CD)-shaped device (2011)
  • Author(s): 古谷俊介
  • Organizer: Single Cell Analysis Summit 2011
  • Place of Presentation: South San Francisco Conference Center (San Francisco, USA)
  • Year and Date: 2011-09-30
• [Presentation] Cell isolation and cell-direct RT-PCR on a compact disk (CD)-shaped device (2011)
  • Author(s): 古谷俊介、永井秀典、高村禅、久保いづみ
  • Organizer: Single Cell Analysis Summit 2011
  • Place of Presentation: South San Francisco Conference Center, San Francisco, USA
  • Year and Date: 2011-09-30
• [Presentation] 簡便な遺伝子検査を目指した微小流体デバイスの開発 (2011)
  • Author(s): 永井秀典
  • Organizer: 電気学会量子ビームによるナノバイオ物理応用技術調査専門委員会
  • Place of Presentation: 産業技術総合研究所四国センター(香川県)
  • Year and Date: 2011-06-30
• [Presentation] 簡便な遺伝子検査を目指した微小流体デバイスの開発 (2011)
  • Author(s): 永井秀典
  • Organizer: 電気学会「量子ビームによるナノバイオ物理応用技術調査専門委員会」
  • Place of Presentation: 香川県、産業技術総合研究所四国センター(招待講演)
  • Year and Date: 2011-06-30
• [Presentation] RT-PCR of GAPDH in Jurkat cells isolated on a Compact Disk (CD)-shaped device (2011)
  • Author(s): 古谷俊介
  • Organizer: ISMM 2011
  • Place of Presentation: Hotel Seoul Kyoyuk Munhwa Hoekwan(Seoul, 韓国)
  • Year and Date: 2011-06-02
• [Presentation] RT-PCR of GAPDH in Jurkat cells isolated on a Compact Disk (CD)-shaped device (2011)
  • Author(s): 古谷俊介、永井秀典、高村禅、久保いづみ
  • Organizer: ISMM 2011
  • Place of Presentation: Hotel Seoul Kyoyuk Munhwa Hoekwan, Seoul, Korea
  • Year and Date: 2011-06-02
• [Presentation] Single cell separation and characterization on compact disk (CD) shaped micro fluidic device (2010)
  • Author(s): 古谷俊介
  • Organizer: The 2010International Chemical Congress of Pacific Basin Societies
  • Place of Presentation: Hawaii convention center(Honolulu, USA)
  • Year and Date: 2010-12-18
• [Presentation] Single cell separation and characterization on compact disk (CD) shaped micro fluidic device. (2010)
  • Author(s): 古谷俊介、永井秀典、高村禅、久保いづみ
  • Organizer: The 2010 International Chemical Congress of Pacific Basin Societies
  • Place of Presentation: Honolulu
  • Year and Date: 2010-12-18
• [Presentation] 簡便な遺伝子検査を目指した微小流体デバイスの開発 (2010)
  • Author(s): 永井秀典
  • Organizer: 産総研オープンラボ 2010
  • Place of Presentation: 産業技術総合研究所(茨城県)
  • Year and Date: 2010-10-15