Development of a digital PCR assay using single cell isolationby large arrays of microwells on a lab-on-a-disk
| Project/Area Number |
22710126
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Microdevices/Nanodevices
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| Research Institution | National Institute of Advanced Industrial Science and Technology |
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Principal Investigator |
NAGAI Hidenori 独立行政法人産業技術総合研究所, 健康工学研究部門, 主任研究員 (40357596)
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| Project Period (FY) |
2010 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | マイクロバイオシステム / 一細胞アッセイ / 細胞アッセイ / PCR / マイクロ流体 / マイクロデバイス / マイクロアレイ / 遺伝子 / 一細胞 / 単離 / 微小分割 |
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| Research Abstract |
Aiming at the early detection of various diseases, such as cancer, a centrifugal disk for a single-cell PCR assay was developed. Instead of the large-sized and expensive cell sorter, the cell isolation based on a simple minute division was achieved byusin
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Report
(4 results)
Research Products
(17 results)
