Elucidation of functional diversity of heterochromatin using ChIP-seq

• Project/Area Number: 22710180
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Genome biology
• Research Institution: Hokkaido University
• Principal Investigator: NAGAO Koji 北海道大学, 大学院・先端生命科学研究院, 助教 (60426575)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2010: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: エピゲノム / ヘテロクロマチン / バイオインフォマティクス / ゲノム

Research Abstract

Heterochromatin plays an essential role for the maintenance of chromosomal integrity and the regulation of cell specific gene expression pattern. In this study, we map heterochromatin-associated proteins throughout genome using ChIP-seq(chromatin immunoprecipitation combined with high-throughput sequencing) method, and elucidate the architecture of human heterochromatin.

Research Products

• [Journal Article] CDK promotes interactions of Sld3 and Drc1 with Cut5 for initiation of DNA replication in fission yeast (2011)
  • Author(s): Fukuura, M., Nagao, K., Obuse, C., Takahashi, T. S., Nakagawa, T., and Masukata, H.
  • Journal Title: Mol. Biol. Cell Volume: 14 Pages: 2620-2633
  • Peer Reviewed
• [Journal Article] CDK promotes interactions of Sld3 and Drc1 with Cut5 for initiation of DNA replication in fission yeast (2011)
  • Author(s): Fukuura M.、 Nagao K.、 Obuse C.、 Takahashi T.S.、 Nakagawa T.、Masukata H.
  • Journal Title: Molecular biology of the cell Volume: 22(14) Issue: 14 Pages: 2620-2633
  • DOI: 10.1091/mbc.e10-12-0995
  • Peer Reviewed
• [Journal Article] Human POGZ modulates dissociation of HP1alpha from mitotic chromosome arms through Aurora B activation (2010)
  • Author(s): Nozawa, R. S., Nagao, K., Masuda, H. T., Iwasaki, O., Hirota, T., Nozaki, N., Kimura, H., and Obuse, C.
  • Journal Title: Nat. Cell Biol Volume: 12 Pages: 719-727
  • Peer Reviewed
• [Journal Article] ヒトHP1結合タンパク質のプロテオーム解析からみえてきたHP1の新機能 (2010)
  • Author(s): 野澤竜介、長尾恒治、小布施力史
  • Journal Title: 実験医学 Volume: 28 Pages: 2983-2986
• [Journal Article] ヒトHP1結合タンパク質POGZは分裂前期のAurora Bキナーゼの染色体腕部での活性化に寄与する (2010)
  • Author(s): 野澤竜介、長尾恒治、小布施力史
  • Journal Title: 細胞工学 Volume: 29 Pages: 868-870
• [Journal Article] Human POGZ modulates dissociation of HP1 alpha from mitotic chromosome arms through Aurora B activation. (2010)
  • Author(s): Nozawa, R.
  • Journal Title: Nat.Cell.Biol. Volume: 12 Pages: 719-727
  • Peer Reviewed
• [Journal Article] Synergistic roles of the proteasome and autophagy for mitochondrial maintenance and chronological lifespan in fission yeast. (2010)
  • Author(s): Takeda, K.
  • Journal Title: Proc.Natl.Acad.Sci.USA. Volume: 107 Pages: 3540-3545
  • Peer Reviewed
• [Presentation] ChIP-seq Analysis of Heterochromatin-associated Histone Modifications in Human Female Cells (2012)
  • Author(s): 長尾恒治、野澤竜介、柴田幸子、木村宏、小布施力史
  • Place of Presentation: 北海道大学
  • Year and Date: 2012-02-24
• [Presentation] ChIP-seq Analysis of Heterochromatin-associated Histone Modifications in Human Female Cells (2012)
  • Author(s): 長尾恒治
  • Organizer: 「哺乳類X染色体不活性化について」研究会
  • Place of Presentation: 北海道大学(札幌市)
  • Year and Date: 2012-02-24
• [Presentation] ChIP-seq Analysis of Heterochromatin-associated Histone Modifications in Human Female Cells (2011)
  • Author(s): 長尾恒治、野澤竜介、柴田幸子、木村宏、小布施力史
  • Organizer: BMB2011第34回日本分子細胞生物学会年会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2011-12-15
• [Presentation] ChIP-seq Analysis of Heterochromatin-associated Histone Modifications in Human Female Cells (2011)
  • Author(s): 長尾恒治
  • Organizer: 第34回日本分子細胞生物学会年会
  • Place of Presentation: パシフィコ横浜(神奈川県)
  • Year and Date: 2011-12-15
• [Presentation] Quantitiative Proteomic Analysis of HP1αInteraction Network-Four Different Interaction Modes of HP1αwith Its Associated Proteins (2011)
  • Author(s): Koji Nagao, Ryu-suke Nozawa, and Chikashi Obuse
  • Organizer: International Symposium Physicochemical Field for Genetic Activities
  • Place of Presentation: 淡路夢舞台国際会議場
  • Year and Date: 2011-01-25
• [Presentation] Quantitative Proteomic Analysis of HP1 α Interaction Network-Four Different Interaction Modes of HP1 α with Its Associated Proteins. (2011)
  • Author(s): 長尾恒治
  • Organizer: International Symposium Physicochemical Field for Genetic Activities
  • Place of Presentation: 淡路市
• [Presentation] 半定量プロテオミクスによるヒトHP1αタンパク質間相互作用ネットワークの解明-HP1は4つの異なる様式でさまざまなタンパク質と相互作用する (2010)
  • Author(s): 長尾恒治、野澤竜介、小布施力史
  • Organizer: BMB2010第33回日本分子細胞生物学会年会第83回日本生化学会大会合同大会
  • Place of Presentation: 神戸国際会議場
  • Year and Date: 2010-12-08
• [Presentation] 判定量プロテオミクスによるヒトHP1αタンパク質間相互作用ネットワークの解明-HP1は4つの異なる様式でさまざまなタンパク質と相互作用する (2010)
  • Author(s): 長尾恒治
  • Organizer: BMB2010
  • Place of Presentation: 神戸市