| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2011: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2010: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
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| Research Abstract |
Simultaneous determinations of 5' and 3' terminal sequences of a mRNA molecule were conducted on budding yeast Saccharomyces cerevisiae with highly parallelized manner. Logarithmically growing cells, and cells before and six times every 2 hours after induction of sporulation were collected. Cloning-free GIS were applied for then, and sequencing was performed on a next-generation sequencer. Construction of a unique database storing transcription initiation and termination position of each budding yeast transcript as a pair is currently underway.
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