High-throughput definition of transcriptional units to grasp global expression states of non-coding RNA in budding yeast cells
| Project/Area Number |
22710182
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Genome biology
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| Research Institution | The University of Tokyo |
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Principal Investigator |
MIURA Fumihito 東京大学, 大学院・理学系研究科, 特任助教 (50447348)
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| Project Period (FY) |
2010 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2011: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2010: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
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| Keywords | トランスクリプトーム / 転写開始点 / 転写終結点 / 出芽酵母 / 対数増殖期 / 胞子形成 / 減数分裂 / ヌクレオソーム |
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| Research Abstract |
Simultaneous determinations of 5' and 3' terminal sequences of a mRNA molecule were conducted on budding yeast Saccharomyces cerevisiae with highly parallelized manner. Logarithmically growing cells, and cells before and six times every 2 hours after induction of sporulation were collected. Cloning-free GIS were applied for then, and sequencing was performed on a next-generation sequencer. Construction of a unique database storing transcription initiation and termination position of each budding yeast transcript as a pair is currently underway.
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Report
(3 results)
Research Products
(13 results)
