Functional analysis of chromatin dynamics during aging by genomic approach

• Project/Area Number: 22710183
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Genome biology
• Research Institution: Niigata University (2011-2012); Tokyo Institute of Technology (2010)
• Principal Investigator: SAKAI Akiko 新潟大学, 医歯学総合研究科, 特任助教 (70532745)
• Research Collaborator: KUWANO Ryozo 新潟大学, 脳研究所, 教授 (20111734) ; SHIRAHIGE Katsuhiko 東京大学, 分子細胞生物学研究所, 教授 (90273854) ; NAKATO Ryuichiro 東京大学, 分子細胞生物学研究所, 助教 (60583044)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥6,110,000 (Direct Cost: ¥4,700,000、Indirect Cost: ¥1,410,000); Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2011: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: ゲノム / ChIP-seq / 転写制御 / クロマチン / 発現制御

Research Abstract

Chromatin structure has fundamental roles in gene expression, which could bere-organized as conditions and circumstances of the cells change. In this study, wehave tried to understand the molecular mechanism and the roles of the structuralchanges in chromatin during aging. ChIP-seq method was applied to identify thebinding sites of chromatin factors throughout the genome in mammalian brain. Ourresults indicated that during the critical period in which the plasticity of the brainincreases, Otx2, the essential transcription factor, and cohesin, the regulator of higherorder chromatin structure, act cooperatively for gene regulation.

Research Products

• [Presentation] Chromatin dynamics duringthe critical period of ocular dominanceplasticity in mouse visual cortex (2012)
  • Author(s): Akiko Sakai
  • Organizer: 遺伝研研究会 Circuit construction in themammalian cerebral cortex: Genetic andimaging approaches
  • Place of Presentation: 三島
• [Presentation] 臨界期におけるマウス視覚野のクロマチン動態の解析 (2012)
  • Author(s): 酒井晶子、中戸隆一郎、桑野良三、白髭克彦、杉山清佳
  • Organizer: 第35回日本神経科学大会
  • Place of Presentation: 名古屋
• [Presentation] 経験依存的な神経回路の可塑性に関わるクロマチン動態の解析 (2012)
  • Author(s): 酒井晶子、桑野良三、杉山清佳
  • Organizer: 第6回日本エピジェネティクス研究会年会
  • Place of Presentation: 東京
• [Presentation] Chromatin dynamics during the critical period of ocular dominance plasticity in mouse visual cortex (2012)
  • Author(s): Akiko Sakai
  • Organizer: 遺伝研研究会Circuit construction in the mammalian cerebral cortex: Genetic and imaging approaches
  • Place of Presentation: 三島