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Analysis of promoter-proximal pausing of transcription by using insertional chromatin immunoprecipitation(iChIP)

Research Project

Project/Area Number 22710185
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Genome biology
Research InstitutionOsaka University

Principal Investigator

FUJITA Toshitsugu  大阪大学, 微生物病研究所, 助教 (10550030)

Project Period (FY) 2010 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywords遺伝子発現調節 / c-fos / iChIP / 転写
Research Abstract

To understand mechanisms of promoter-proximal pausing of transcription, we aimed to specifically isolate the promoter-proximal region of the c-fos gene and to identify molecules(proteins, DNA, RNA) binding to the isolated region by using insertional chromatin immunoprecipitation(iChIP). As a step of iChIP, we constructed a targeting vector for insertion of the binding elements of LexA, a bacterial DNA-binding protein, around the c-fos promoter-proximal region. We are now conducting gene targeting with the vector. On the other hand, we optimized iChIP for isolation of low copy number of a target genomic region and identification of proteins and RNA binding to the region.

Report

(3 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report
  • Research Products

    (11 results)

All 2011 2010 Other

All Journal Article (3 results) (of which Peer Reviewed: 3 results) Presentation (2 results) Remarks (3 results) Patent(Industrial Property Rights) (3 results) (of which Overseas: 2 results)

  • [Journal Article] Direct identification of insulator components by insertional chromatin immunoprecipitation2011

    • Author(s)
      Fujita, T. and Fujii, H
    • Journal Title

      PLoS ONE

      Volume: 6 Issue: 10 Pages: e26109-e26109

    • DOI

      10.1371/journal.pone.0026109

    • Related Report
      2011 Annual Research Report 2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Species-specific 5'-genomic structure and multiple transcription start sites in the chicken Pax5 gene2011

    • Author(s)
      Fujita, T. and Fujii, H
    • Journal Title

      Gene

      Volume: 477 Issue: 1-2 Pages: 24-31

    • DOI

      10.1016/j.gene.2011.01.008

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Species-specific 5'-genomic structure and multiple transcription start sites in the chicken Pax5 gene.2011

    • Author(s)
      藤田敏次
    • Journal Title

      Gene

      Volume: (in press)

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Presentation] 挿入的クロマチン免疫法を利用したインスレーター分子の同定2011

    • Author(s)
      藤田敏次、藤井穂高
    • Organizer
      第34回日本分子生物学会年会
    • Place of Presentation
      パシフィコ横浜、神奈川県
    • Year and Date
      2011-12-16
    • Related Report
      2011 Final Research Report
  • [Presentation] 挿入的クロマチン免疫法を利用したインスレーター分子の同定2011

    • Author(s)
      藤田敏次
    • Organizer
      日本分子生物学会
    • Place of Presentation
      パシフィコ横浜(神奈川県)
    • Year and Date
      2011-12-16
    • Related Report
      2011 Annual Research Report
  • [Remarks] ホームページ

    • URL

      http://www.biken.osaka-u.ac.jp/lab/microimm/fujii/index.html

    • Related Report
      2011 Final Research Report
  • [Remarks]

    • URL

      http://www.biken.osaka-u.ac.jp/lab/microimm/fujii/index.html

    • Related Report
      2011 Annual Research Report
  • [Remarks]

    • URL

      http://www.biken.osaka-u.ac.jp/lab/microimm/fujii/index.html

    • Related Report
      2010 Annual Research Report
  • [Patent(Industrial Property Rights)] 特定ゲノム領域の単離方法2010

    • Inventor(s)
      藤井穂高、藤田敏次
    • Industrial Property Rights Holder
      大阪大学
    • Filing Date
      2010-08-20
    • Related Report
      2011 Final Research Report
    • Overseas
  • [Patent(Industrial Property Rights)] 特定ゲノム領域の単離方法2010

    • Inventor(s)
      藤井穂高, 藤田敏次
    • Industrial Property Rights Holder
      大阪大学
    • Filing Date
      2010-08-20
    • Related Report
      2010 Annual Research Report
  • [Patent(Industrial Property Rights)] 特定ゲノム領域の単離方法2010

    • Inventor(s)
      藤井穂高, 藤田敏次
    • Industrial Property Rights Holder
      大阪大学
    • Filing Date
      2010-08-20
    • Related Report
      2010 Annual Research Report
    • Overseas

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Published: 2010-08-23   Modified: 2016-04-21  

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