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Absolute quantification of human proteome by information based quantitative proteomics

Research Project

Project/Area Number 22710186
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Genome biology
Research InstitutionKyushu University

Principal Investigator

MATSUMOTO Masaki  九州大学, 生体防御医学研究所 (60380531)

Project Period (FY) 2010 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2010: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Keywordsプロテオーム / プロテオミクス / 絶対定量 / ネットワーク解析 / システム生物学
Research Abstract

Due to the huge dynamic range of proteins amount, the detection of proteins expressed in low copy number by conventional proteomics based on mass-spectrometry is a major challenge. To solve this issue, we developed novel method to detect and quantitate human proteins by the combination of MRM, target proteomics and recombinant proteins generated from cDNA library. Using this system, we obtained information about the absolute expression level of many proteins including low abundant proteins that were difficult to detect.

Report

(3 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report
  • Research Products

    (22 results)

All 2012 2011 2010 Other

All Journal Article (14 results) (of which Peer Reviewed: 14 results) Presentation (6 results) Remarks (2 results)

  • [Journal Article] Proteome-wide identification of ubiquitylation sites by conjugation of engineered lysine-less ubiquitin2012

    • Author(s)
      Oshikawa K, Matsumoto M, Oyamada K, Nakayama KI.
    • Journal Title

      J. Proteome Res

      Volume: 11 Issue: 2 Pages: 796-807

    • DOI

      10.1021/pr200668y

    • Related Report
      2011 Annual Research Report 2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Genetic reevaluation of the role of F-box proteins in cyclin D1 degradation2012

    • Author(s)
      Kanie T, et al
    • Journal Title

      Molecular and Cellular Biology

      Volume: 32 Issue: 3 Pages: 590-605

    • DOI

      10.1128/mcb.06570-11

    • Related Report
      2011 Annual Research Report 2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Chk1 phosphorylates the tumor suppressor Mig-6, regulating the activation of EGF signaling2012

    • Author(s)
      Liu N., Matsumoto M., Kitagawa K., Kotake Y., Suzuki S., Shirasawa S., Nakayama KI, Nakanishi M., Niida H., Kitagawa M.
    • Journal Title

      EMBO Journal

      Volume: (In press)

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Protrudin serves as an adaptor molecule that connects KIF5 and its cargoes in vesicular transport during process formation2011

    • Author(s)
      Matsuzaki, F., Shirane, M., Matsumoto, M., & Nakayama, K.I
    • Journal Title

      Mol. Biol. Cell

      Volume: 22 Issue: 23 Pages: 4602-4620

    • DOI

      10.1091/mbc.e11-01-0068

    • Related Report
      2011 Annual Research Report 2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Functional analyses of the activation loop of phototropin2 in Arabidopsis2011

    • Author(s)
      Inoue S, Matsushita T, Tomokiyo Y, Matsumoto M, Nakayama KI, Kinoshita T, Shimazaki K.
    • Journal Title

      Plant Physiology

      Volume: 156 Issue: 1 Pages: 117-128

    • DOI

      10.1104/pp.111.175943

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] TRIM8 regulates Nanog via Hsp90 beta-mediated nuclear translocation of STAT3 in embryonic stem cells2011

    • Author(s)
      Okumura, F., Okumura, A.J., Matsumoto, M., Nakayama, K.I. and Hatakeyama, S.
    • Journal Title

      BBA-Mol. Cell Res.

      Volume: 1813 Issue: 10 Pages: 1784-1792

    • DOI

      10.1016/j.bbamcr.2011.05.013

    • Related Report
      2011 Annual Research Report 2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Proteome of ubiquitin/MVB pathway : possible involvement of iron-induced ubiquitylation of transferrin receptor in lysosomal degradation2011

    • Author(s)
      Tachiyama R, Ishikawa D, Matsumoto M, Nakayama KI, Yoshimori T, Yokota S, Himeno M, Tanaka Y, Fujita H
    • Journal Title

      Genes Cells

      Volume: 16 Issue: 4 Pages: 448-66

    • DOI

      10.1111/j.1365-2443.2011.01499.x

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Proteome of ubiquitin/MVB pathway : possible involvement of iron-induced ubiquitylation of transferrin receptor in lysosomal degradation.2011

    • Author(s)
      Tachiyama R, Ishikawa D, Matsumoto M, Nakayama KI, Yoshimori T, Yokota S, Himeno M, Tanaka Y, Fujita H.
    • Journal Title

      Genes Cells

      Volume: 16 Pages: 448-466

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Human proteins that specifically bind to 8-oxoguanine-containing RNA and their responses to oxidative stress2010

    • Author(s)
      Hayakawa H, Fujikane A, Ito R, Matsumoto M, Nakayama KI, Sekiguchi M.
    • Journal Title

      Biochem Biophys Res. Commun

      Volume: 403 Issue: 2 Pages: 220-224

    • DOI

      10.1016/j.bbrc.2010.11.011

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Comparative analysis of human SRC-family kinase substrate specificity in vitro2010

    • Author(s)
      Takeda H, Kawamura Y, Miura A, Mori M, Wakamatsu A, Yamamoto J., Isogai T., Matsumoto M, Nakayama KI., Natsume T., Nomura N., Goshima N.
    • Journal Title

      J. Proteome Res

      Volume: 9 Issue: 11 Pages: 5982-5993

    • DOI

      10.1021/pr100773t

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Phosphorylation of the chromodomain changes the binding specificity of Cbx2 for methylated histone H32010

    • Author(s)
      Hatano A, Matsumoto M, Higashinakagawa T, Nakayama KI.
    • Journal Title

      Biochem Biophys Res. Commun

      Volume: 397 Issue: 1 Pages: 93-99

    • DOI

      10.1016/j.bbrc.2010.05.074

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Human proteins that specifically bind to 8-oxoguanine-containing RNA and their responses to oxidative stress.2010

    • Author(s)
      Hayakawa H, Fujikane A, Ito R, Matsumoto M, Nakayama KI, Sekiguchi M
    • Journal Title

      Biochem Biophys Res Commun

      Volume: 403 Pages: 220-224

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Comparative analysis of human SRC-family kinase substrate specificity in vitro2010

    • Author(s)
      Takeda H., Kawamura Y., Miura A., Mori M., Wakamatsu A., Yamamoto J., Isogai T., Matsumoto M., Nakayama KI., Natsume T., Nomura N., Goshim N.
    • Journal Title

      J Proteome Res

      Volume: 9 Pages: 5982-93

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Phosphorylation of the chromodomain changes the binding specificity of Cbx2 for methylated histone H3.2010

    • Author(s)
      Hatano A, Matsumoto M, Higashinakagawa T, Nakayama KI
    • Journal Title

      Biochem Biophys Res Commun.

      Volume: 397 Pages: 93-99

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Presentation] MS定量解析および大規模データ解析2011

    • Author(s)
      松本雅記
    • Organizer
      日本生化学会
    • Place of Presentation
      京都・国立国際会館
    • Year and Date
      2011-09-13
    • Related Report
      2011 Final Research Report
  • [Presentation] MS定量解析および大規模データ解析2011

    • Author(s)
      松本雅記
    • Organizer
      日本生化学会
    • Place of Presentation
      京都・国立国際会館(招待講演)
    • Year and Date
      2011-09-13
    • Related Report
      2011 Annual Research Report
  • [Presentation] 定量プロテオミクスによる生命科学研究の新展開2011

    • Author(s)
      松本雅記、中山敬一
    • Organizer
      日本ヒトプロテオーム学会
    • Place of Presentation
      新潟・朱鷺メッセ
    • Year and Date
      2011-07-19
    • Related Report
      2011 Final Research Report
  • [Presentation] 定量プロテオミクスによる生命科学研究の新展開2011

    • Author(s)
      松本雅記、中山敬一
    • Organizer
      日本ヒトプロテオーム学会
    • Place of Presentation
      新潟・朱鷺メッセ(招待講演)
    • Year and Date
      2011-07-19
    • Related Report
      2011 Annual Research Report
  • [Presentation] 定量プロテオミクスが拓く生命科学研究の新展開2010

    • Author(s)
      松本雅記
    • Organizer
      第33回日本分子生物学会年会・第83回日本生化学会大会合同大会
    • Place of Presentation
      神戸ポートアイランド
    • Year and Date
      2010-12-08
    • Related Report
      2011 Final Research Report 2010 Annual Research Report
  • [Presentation] 情報基盤ターゲット・プロテオミクスを用いたヒト・プロテオームの絶対定量2010

    • Author(s)
      松本雅記、五島直樹、夏目徹、中山敬一
    • Organizer
      日本プロテオーム学会
    • Place of Presentation
      東京ベイホテル東急
    • Year and Date
      2010-07-27
    • Related Report
      2011 Final Research Report 2010 Annual Research Report
  • [Remarks]

    • URL

      http://www.bioreg.kyushu-u.ac.jp/saibou/index.html

    • Related Report
      2011 Annual Research Report
  • [Remarks]

    • URL

      http://www.bioreg.kyushu-u.ac.jp/saibou/index.html

    • Related Report
      2010 Annual Research Report

URL: 

Published: 2010-08-23   Modified: 2016-04-21  

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