| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2010: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
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| Research Abstract |
Due to the huge dynamic range of proteins amount, the detection of proteins expressed in low copy number by conventional proteomics based on mass-spectrometry is a major challenge. To solve this issue, we developed novel method to detect and quantitate human proteins by the combination of MRM, target proteomics and recombinant proteins generated from cDNA library. Using this system, we obtained information about the absolute expression level of many proteins including low abundant proteins that were difficult to detect.
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