| Project/Area Number |
22710187
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Genome biology
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| Research Institution | Kyushu University |
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Principal Investigator |
MIURA Daisuke 九州大学, 先端融合医療レドックスナビ研究拠点, 准教授 (40532627)
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| Project Period (FY) |
2010 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2010: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | メタボローム / 質量分析 / マトリックス支援レーザー脱離イオン化 / メタボロミクス / イメージング / マトリックス支援レーザー脱離イオン化法 / 代謝動態 |
|---|
| Research Abstract |
We successfully showed the applicability of a matrix-assisted laser desorption ionization mass spectrometry(MALDI-MS) system for getting chemically diverse metabolite profiles on a single-mammalian cell. This ultrahighly sensitive MALDI-MS technique enabled a spatially resolved detection of a broad range of metabolites including nucleotides, cofactors, phosphorylated sugars, amino acids, lipids, and carboxylic acids with their unique distributions. A combination of MS imaging and metabolic pathway analysis visualized a spatiotemporal behavior of metabolites in the central metabolic pathway regulated by an ischemia reperfusion in rat brain. Furthermore, we developed a novel strategy for determining the elemental composition of organic compounds using the peak ratio of isotopic fine structure observed by ultrahigh mass resolution mass spectrometric data.
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