| Project/Area Number |
22710189
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Genome biology
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| Research Institution | The Institute of Physical and Chemical Research |
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Principal Investigator |
KAWAJI Hideya 独立行政法人理化学研究所, 小分子RNA連携ユニット, ユニットリーダー (20525406)
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|---|
| Project Period (FY) |
2010 – 2011
|
| Project Status |
Completed (Fiscal Year 2011)
|
| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2010: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | miRNA / primary transcript / promoter / transcriptome / transcriptional regulation / small RNA / next generation sequencer / primary miRNA / non-coding RNA / プロモータ / 転写開始点 / RNA silencing / RNA干渉 / primary miRN / ncRNA |
|---|
| Research Abstract |
We performed siRNA knockdown experiments against factors contributing the miRNA biogenesis, and profiled nuclear RNA with high-throughput sequencing technologies. With HeliScopeCAGE that profile TSS activities quantitatively, we identified 24 promoters on sixty miRNAs. With RNA-seq, we identified more than half of the 24 miRNA promoters.
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