RNAi screening for genes involved in differentiation of embryonic stem cells into primordial germ cells.

Research Project

Project/Area Number	22710197
Research Category	Grant-in-Aid for Young Scientists (B)
Allocation Type	Single-year Grants
Research Field	System genome science
Research Institution	Tohoku University
Principal Investigator	MAEDA Ikuma 東北大学, 加齢医学研究所, 助教 (20376560)
Project Period (FY)	2010 – 2011
Project Status	Completed (Fiscal Year 2011)
Budget Amount *help	¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000) Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000) Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywords	発生分化 / 生殖細胞 / ゲノム / ES細胞
Research Abstract	We identified Max as a repressor of germ-cell-related genes in embryonic stem cells (ESCs)via RNAi screen. Transcriptome analysis revealed that Max knockdown (KD)in ESCs resulted in selective, global derepression of germ-cell-specific genes. Max interacted with histone H3K9 methyltransferases and associated with the promoter regions of germ-cell-specific genes in ESCs. In addition, Max-KD resulted in a decrease in histone H3K9 dimethylation at those promoter regions.