Research Project
Grant-in-Aid for Young Scientists (B)
DNA methylation dynamically affects the expression of downstream genes. To obtain the fundamental technologies of site-specific changes of modified DNA, I first established the methylated DNA binding domain capture and next-generation sequencing(MBD-seq) and observed the DNA methylation status of human fibroblasts and monocytes. I also established a simple and flexible episomal vector-based method called MoCEV(Modified Cytosine in Episomal Vector) to monitor the status of cytosine modification of any desired DNA fragments. I observed enhanced expression of fluorescent marker protein controlled by DNA methylated promoter upon the treatment of 5-aza-2'-deoxycytidine in a dose dependent manner. I believe that the established methods and findings in this project may help to develop new methods of site-specific changes of modified DNA.
All 2011
All Presentation (1 results)