Construction of dynamic transcriptional regulatory netowork analysis system

• Project/Area Number: 22710201
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: System genome science
• Research Institution: The Institute of Physical and Chemical Research
• Principal Investigator: TOMARU Yasuhiro 独立行政法人理化学研究所, LSA要素技術開発ユニット, 研究員 (30381528)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2011: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000); Fiscal Year 2010: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: Matrix RNAi(マトリックスRNAi) / 転写制御ネットワーク / ダイナミック転写制御ネットワーク / 間葉系幹細胞(MSC) / Matrix RNAi / 転写制御 / Cell Dynamics

Research Abstract

Human mesenchymal stem cell(MSC) can differentiate to adipocyte and osteoblast. Aim of this research is experimental analysis system construction for dynamic transcriptional regulatory network using this MSC differentiation model.
Three achievements indicated below could be completed. 1) 12 time points of genome wide gene expression profile of adipogenic and osteogenic from MSC, 2) Adipogenic or osteogenic specific transcription foctor list, 3) specialization of gene knock down system with siRNA and lentivirus shRNA expression vector for MSCs.
However construction of conditional shRNA expression system could not succeed. And construction of dynamic network analysis system was not finished completely in this term.