In vitro selection of non-standard cyclic peptides that regulate maturation of miRNAs

• Project/Area Number: 22710210
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Living organism molecular science
• Research Institution: The University of Tokyo
• Principal Investigator: TAKAYUKI Katoh 東京大学, 大学院・理学系研究科, 助教 (90567760)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2011: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2010: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: ケミカルバイオロジー / マイクロRNA / 特殊ペプチド / mRNAディスプレイ / FITシステム / 遺伝暗号リプログラミング / 分子標的医薬 / ペプチド医薬 / 特殊環状ペプチド

Research Abstract

In this proect, we tried to develop non-standard cyclic peptides that can inhibit the maturation of miRNA precursor by using RaPID display method, which is a combination of genetic code reprogramming and mRNA display. So far, we have successfully obtained several cyclic peptides that have binding affinity to miR-21 precursor. Evaluations of specificity and binding affinity of the peptides are under way.

Research Products

• [Journal Article] Charging of tRNAs using ribozymes and selection of cyclic peptides containing thioethers (2012)
  • Author(s): Reid PC, Goto Y, Katoh TSuga H
  • Journal Title: Methods Mol Biol Volume: 805 Pages: 335-348
• [Journal Article] Selective thioether macrocyclization of peptides having the N-terminal 2-chloroacetyl group and competing two or three cysteine residues in translation (2012)
  • Author(s): Iwasaki K, Goto Y, Katoh T, Suga H
  • Journal Title: Org Biomol Chem
  • Peer Reviewed
• [Journal Article] Selective thioether macrocyclization of peptides having the N-terminal 2-chloroacetyl group and competing two or three cysteine residues in translation (2012)
  • Author(s): Kazuhiro Iwasaki, Yuki Goto, Takayuki Katoh, Hiroaki Suga
  • Journal Title: Org.Biomol.Chem. Volume: 10 Issue: 30 Pages: 5783-5786
  • DOI: 10.1039/c2ob25306b
  • Peer Reviewed
• [Journal Article] Flexizymes for genetic code reprogramming (2011)
  • Author(s): Goto Y, Katoh T, Suga H
  • Journal Title: Nat Protoc Volume: 6 Pages: 779-790
  • Peer Reviewed
• [Journal Article] Ribosomal synthesis of backbone macro cyclic peptides (2011)
  • Author(s): Katoh TGoto Y, Reza MS, Suga H
  • Journal Title: Chem Commun Volume: 47 Pages: 9946-9958
  • Peer Reviewed
• [Journal Article] Natural product-like macro cyclic N-methyl-peptide inhibitors against ubiquitin ligase uncovered from ribosome-expressed de novo library (2011)
  • Author(s): Yamagishi Y, ShoI, Miyagawa S, Kawakami T, Katoh T, Goto Y, Suga H
  • Journal Title: Chem Biol Volume: 18 Pages: 1562-1570
  • Peer Reviewed
• [Journal Article] Drug discovery of non-standard peptide with genetic code reprogramming (2011)
  • Author(s): 山口淳, 加藤敬行, 菅裕明
  • Journal Title: Drug Delivery System Volume: 26 Issue: 6 Pages: 584-592
  • DOI: 10.2745/dds.26.584
  • NAID: 130002100330
  • ISSN: 0913-5006, 1881-2732
• [Journal Article] Flexizymes for genetic code reprogramming (2011)
  • Author(s): Y.Goto, T.Katoh, H.Suga
  • Journal Title: Nature Protocols Volume: 6 Issue: 6 Pages: 779-790
  • DOI: 10.1038/nprot.2011.331
  • Peer Reviewed
• [Journal Article] Ribosomal synthesis of backbone macrocyclic peptides (2011)
  • Author(s): T.Katoh, Y.Goto, S.Raza, H.Suga*
  • Journal Title: Chemical Communication Volume: 47 Issue: 36 Pages: 9946-9958
  • DOI: 10.1039/c1cc12647d
  • Peer Reviewed
• [Journal Article] Charging of tRNAs using ribozymes and selection of cyclic peptides containing thioethers (2011)
  • Author(s): Reid PC, Goto Y, Katoh T, Suga H
  • Journal Title: Methods Mol Biol. Volume: 805 Pages: 335-348
• [Journal Article] Natural product-like macrocyclic N-methyl-peptide inhibitors against a ubiquitin ligase uncovered from a ribosome-expressed de novo library (2011)
  • Author(s): Y.Yamagishi, I.Shoji, S.Miyagawa, T.Kawakami, T.Katoh, Y.Goto, H.Suga*
  • Journal Title: Chemistry & Biology Volume: 18 Issue: 12 Pages: 1562-1570
  • DOI: 10.1016/j.chembiol.2011.09.013
  • Peer Reviewed
• [Journal Article] Flexizymes for genetic code reprogramming (2011)
  • Author(s): Yuki Goto, Takayuki Katoh, Hiroaki Suga
  • Journal Title: Nature Protocols Volume: 6 Pages: 779-790
  • Peer Reviewed
• [Presentation] ヒストンリシン脱メチル化酵素JMJD2Aに対する特殊環状ペプチド阻害剤のセレクション及び活性評価 (2012)
  • Author(s): 小嶋達矢、加藤敬行、菅裕明
  • Organizer: 日本化学会第92春季年会
  • Place of Presentation: 慶應義塾大学
  • Year and Date: 2012-03-27
• [Presentation] ヒストンリシン脱メチル化酵素JMJD2Aに対する特殊環状ペプチド阻害剤のセレクション及び活性評価 (2012)
  • Author(s): 小嶋達矢
  • Organizer: 日本化学会第92春季年会
  • Place of Presentation: 神奈川(慶應大学)
  • Year and Date: 2012-03-27
• [Presentation] In vitroセレクションによる小分子結合性small non-coding RNAの探索 (2011)
  • Author(s): 寺坂尚紘、加藤敬行、菅裕明
  • Organizer: 第34回日本分子生物学会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2011-12-15
• [Presentation] in vitroセレクションによる小分子結合性small non-coding RNAの探索 (2011)
  • Author(s): 寺坂尚紘
  • Organizer: 第34回日本分子生物学会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2011-12-15
• [Presentation] In vitro selection of macrocyclic peptide inhibitors against various target proteins by means of ribosome-expressed peptide library (2011)
  • Author(s): 加藤敬行
  • Organizer: 第34回日本分子生物学会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2011-12-13
• [Presentation] In vitro selection of macrocyclic peptide inhibitors against various target proteins by means of ribosome-expressed peptide library (2011)
  • Author(s): 加藤敬行
  • Organizer: 第34回日本分子生物学会
  • Place of Presentation: パシフィコ横浜(招待講演)
  • Year and Date: 2011-12-13
• [Presentation] 特殊環状ペプチドライブラリを用いた分子標的医薬の開発 (2011)
  • Author(s): 加藤敬行
  • Organizer: 第11回日本蛋白質科学会
  • Place of Presentation: 大阪・ホテル阪急エキスポパーク
  • Year and Date: 2011-06-07
• [Presentation] 特殊環状ペプチドライブラリを用いた分子標的医薬の開発 (2011)
  • Author(s): 加藤敬行
  • Organizer: 第11回日本蛋白質科学会
  • Place of Presentation: 大阪・ホテル阪急エキスポパーク(招待講演)
  • Year and Date: 2011-06-07
• [Book] 新しい薬学事典(ペプチド創薬の章) (2011)
  • Author(s): 加藤敬行、渡辺達哉、人見梓、青木由一郎、菅裕明
  • Publisher: 朝倉書店(刊行確定)
• [Remarks]
  • URL: http://www.chem.s.u-tokyo.ac.jp/users/bioorg/index.html