Development of a method for purification of membrane proteins by photocleavable linkers.

• Project/Area Number: 22710216
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Living organism molecular science
• Research Institution: Osaka University
• Principal Investigator: HARA Toshiaki 大阪大学, 大学院・理学研究科, 特任講師 (90528233)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 天然物有機化学 / 膜タンパク質 / ペプチド化学合成 / 構造生物学 / 膜蛋白質 / 光分解リンカー

Research Abstract

We has developed a novel class of photocleavable linkers useful in a purification of a chemically-synthesized membrane peptides and proteins.

Research Products

• [Journal Article] New Redox Linker For The Preparation of Membrane Protein "Subunitc of Human FoF_1-ATP Synthase (2011)
  • Author(s): Hara, T.; Yajima, N.; Aimoto, S.
  • Journal Title: Peptides Volume: 2010 Pages: 199-200
  • Peer Reviewed
• [Journal Article] New Redox Linker For The Preparation of Membrane Protein" Subunit c of Human F_OF_1-ATP Synthase" (2010)
  • Author(s): Hara, T.; Yajima, N.; Aimoto, S.
  • Journal Title: Peptides Pages: 199-199
  • Peer Reviewed
• [Journal Article] Chemical Synthesis of the Functional Domain of Voltage-Gated Proton Channel (2010)
  • Author(s): Nakamura, K.; Hara, T.; Akai, Y.; Sato, T.; Kawakami, T.; Aimoto, S.
  • Journal Title: Peptides
  • NAID: 10028237752
  • Peer Reviewed
• [Journal Article] Synthetic studies of membrane proteins : the subunit c of human F1Fo-ATP synthase and voltage-gated proton channel(78-222). Ed. Rui Wang and Kai-Rong Wang. (2010)
  • Author(s): Saburo Aimoto, Ken' ichiro Nakamura, Takeshi Sato, Toru Kawakami, and Toshiaki Hara
  • Journal Title: Peptides, 11^<th> CPS2010 Pages: 47-53
• [Presentation] Membrane-induced folding reaction of disulfide-rich peptides (2011)
  • Author(s): Toshiaki HARA
  • Organizer: International ERATO Symposium on Lipid Structures in and around Proteins
  • Place of Presentation: 大阪、吹田
  • Year and Date: 2011-11-14
• [Presentation] (2011)
  • Author(s): 原利明, 矢嶋奈央, Euis Maras Purwati, 相本三郎
  • Organizer: 日本化学会第91回春季年会
  • Place of Presentation: 神奈川、横浜、口頭発表
  • Year and Date: 2011-03-11
• [Presentation] "New linker system for the preparation of membrane proteins." IPR Seminar (2010)
  • Author(s): Toshiaki Hara
  • Organizer: The New Trend of Peptide Science in Asia and Oceania
  • Place of Presentation: 大阪、吹田、口頭発表
  • Year and Date: 2010-10-10
• [Presentation] New Redox Linker For The Preparation Of Membrane Protein : Subunit c of Human F_1F_o-ATP Synthase (2010)
  • Author(s): Toshiaki Hara, Nao Yajima, and Saburo Aimoto
  • Organizer: The 5th International Peptide Symposium
  • Place of Presentation: 京都、ポスター発表
  • Year and Date: 2010-10-09
• [Presentation] Chemical Synthesis of the Functional Domain of Voltage-Gated Proton Channel (2010)
  • Author(s): Ken' ichiroh Nakamura, Toshiaki Hara, Yuichi Akai, Takeshi Sato, Toru Kawakami, Saburo Aimoto
  • Organizer: The 5th International Peptide Symposium
  • Place of Presentation: 京都、ポスター発表
  • Year and Date: 2010-10-09
• [Presentation] the subunit c of human F1Fo-ATP synthase and voltage-gated proton channel(78-222) (2010)
  • Author(s): Saburo Aimoto*, Ken' ichiro Nakamura, Takeshi Sato, Toru Kawakami, and Toshiaki Hara
  • Organizer: Synthetic studies of membrane proteins
  • Place of Presentation: 中国、蘭州、口頭発表
• [Remarks]
  • URL: http://www.protein.osaka-u.ac.jp/organic/research/research.html
• [Remarks]
  • URL: http://www.protein.osaka-u.ac.jp/organic/research/research.html