In vitro selection of fluorescent RNAs for in vivo imaging

• Project/Area Number: 22710217
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Living organism molecular science
• Research Institution: Osaka University
• Principal Investigator: HAGIHARA Masaki 大阪大学, 産業科学研究所, 助教 (40403000)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: RNAアプタマー / 蛍光タンパク質 / 蛍光性RNA

Research Abstract

In vitro selection provides one of the most powerful strategies for obtaining functional RNA molecules. In this project, we tried to obtain RNA receptors against the synthetic chromophores by in vitro selection to investigate whether synthetic RNA receptor-chromophore complexes can emit fluorescence as other fluorescent proteins do. The selection and evolution technique has produced RNA aptamers that specifically bind small molecules and emit fluorescence only when RNA aptamers bound to synthetic chromophores

Research Products

• [Journal Article] A small molecule regulates hairpin structures in d(CGG) trinucleotide repeats (2012)
  • Author(s): Masaki Hagihara, Hanping He, Maki Kimura, Kazuhiko Nakatani
  • Journal Title: Bioorganic & Medicinal Chemistry Letters Volume: 22 Pages: 2000-2003
  • Peer Reviewed
• [Journal Article] Small Molecule Modulates Hairpin Structures in CAG Trinucleotide Repeats (2011)
  • Author(s): Masaki Hagihara, He Hanping, Kazuhiko Nakatani
  • Journal Title: ChemBioChem Volume: 12 Pages: 1686-1689
  • Peer Reviewed
• [Journal Article] Ligand-Assisted Complex of Two DNA Hairpin Loops (2011)
  • Author(s): Changfeng Hong, Masaki Hagihara, Kazuhiko Nakatani
  • Journal Title: Angewandte Chemie International Edition Volume: 50 Pages: 4390-4393
  • Peer Reviewed
• [Journal Article] Antisense-induced guanine quadruplexes inhibit reverse transcription by HIV-1 reverse transcriptase (2010)
  • Author(s): Masaki Hagihara, Lisa Yamauchi, Akiko Seo, Keisuke Yoneda, Mayo Senda, Kazuhiko Nakatani
  • Journal Title: Journal of the American Chemical Society Volume: 132 Pages: 11171-11178
  • Peer Reviewed
• [Journal Article] A reverse transcriptase stop assay revealed diverse quadruplex formations in UTRs in mRNA (2010)
  • Author(s): Masaki Hagihara, Keisuke Yoneda, Hiroaki Yabuuchi, Yasushi Okuno, Kazuhiko Nakatani
  • Journal Title: Bioorganic & Medicinal Chemistry Letters Volume: 20 Pages: 2350-2353
  • Peer Reviewed
• [Journal Article] Structure-activity studies on the fluorescent indicator in the displacement assay for the screening of small molecules binding to RNA
  • Author(s): Shiori Umemoto, Seongwang Im, Jinhua Zhang, Masaki Hagihara, Asako Murata, Yasue Harada, Takeo Fukuzumi, Takahiro Wazaki, Shin-ichi Sasaoka, and Kazuhiko Nakatani
  • Journal Title: Chemistry European Journal Volume: (in press)
  • Peer Reviewed
• [Presentation] グアニン修飾アンチセンス核酸を利用したグアニン四重鎖構造制御法 (2012)
  • Author(s): 萩原正規、中谷和彦
  • Organizer: 日本化学会第92春季年会
  • Place of Presentation: 慶應義塾大学日吉キャンパス
  • Year and Date: 2012-03-28
• [Presentation] 新奇アンチセンス核酸を利用したグアニン四重鎖構造制御法 (2011)
  • Author(s): 萩原正規、中谷和彦
  • Organizer: 第5回バイオ関連化学シンポジウム
  • Place of Presentation: つくば国際会議場「エポカルつくば」
  • Year and Date: 2011-09-13
• [Presentation] 新奇アンチセンス核酸を利用したグアニン四重鎖構造制御法 (2011)
  • Author(s): 萩原正規、中谷和彦
  • Organizer: 第5回バイオ関連化学シンポジウム
  • Place of Presentation: つくば国際会議場「エポカルつくば」(茨城)
  • Year and Date: 2011-09-13
• [Presentation] 新奇アンチセンス核酸を利用したRNA中のグアニン四重鎖構造の制御 (2011)
  • Author(s): 萩原正規、中谷和彦
  • Organizer: アンチセンス.遺伝子.デリバリーシンポジウム2011
  • Place of Presentation: 大阪大学コンベンションセンター
  • Year and Date: 2011-09-01
• [Presentation] 新奇アンチセンス核酸を利用したRNA中のグアニン四重鎖構造の制御 (2011)
  • Author(s): 萩原正規、中谷和彦
  • Organizer: アンチセンス・遺伝子・デリバリーシンポジウム2011
  • Place of Presentation: 大阪大学コンベンションセンター(大阪)
  • Year and Date: 2011-09-01
• [Presentation] Antisense-Induced G-Quadruplex Structures Interfere with Reverse Transcription by HIV-1 Reverse Transcriptase (2011)
  • Author(s): Masaki Hagihara, Kazuhiko Nakatani
  • Organizer: RNA 2011, the 16th Annual Meeting of the RNA Society
  • Place of Presentation: 京都国際会議場(京都)
  • Year and Date: 2011-06-16
• [Presentation] Antisense-Induced G-Quadruplex Structures Interfere with Reverse Transcription by HIV-1 Reverse Transcriptase (2011)
  • Author(s): Masaki Hagihara, Kazuhiko Nakatani
  • Organizer: the 16th Annual Meeting of the RNA Society
  • Place of Presentation: 京都国際会議場
  • Year and Date: 2011-06-15
• [Presentation] アンチセンス核酸を利用したグアニン四重鎖構造制御法 (2011)
  • Author(s): 萩原正規、中谷和彦
  • Organizer: 日本化学会第91春季年会
  • Place of Presentation: 神奈川大学
  • Year and Date: 2011-03-26
• [Presentation] In vitro selection of RNA aptamers that can bind to GFP chromophore (2010)
  • Author(s): Masaki Hagihara, Keisuke Yoneda, and Kazuhiko Nakatani
  • Organizer: PACIFICHEM 2010
  • Place of Presentation: ハワイコンベンションセンター
  • Year and Date: 2010-12-19
• [Presentation] Reverse transcriptase stop assay revealed diverse quadruplex formations in mRNA (2010)
  • Author(s): Masaki Hagihara and Kazuhiko Nakatani
  • Organizer: PACIFICHEM2010
  • Place of Presentation: 日ハワイコンベンションセンター
  • Year and Date: 2010-12-09
• [Presentation] Kazuhiko Nakatani Antisense-induced guanine quadruplexes interfere with reverse transcription by HIV-1 reverse transcriptase (2010)
  • Author(s): Masaki Hagihara, Lisa Yamauchi, Akiko Seo, Keisuke Yoneda, Mayo Senda
  • Organizer: ISNAC2010
  • Place of Presentation: 横浜はまぎんホール・ヴィアマーレ
  • Year and Date: 2010-11-10
• [Presentation] Quadruplexes in Untranslated Regions in mRNA Modulates Translation (2010)
  • Author(s): Masaki Hagihara
  • Organizer: The 4th HiPep-Okinawa International Workshop Program
  • Place of Presentation: 沖縄産業支援センター
  • Year and Date: 2010-07-09
• [Presentation] RNA Quadruplexes in Untranslated Regions in mRNA Modulates Translation (2010)
  • Author(s): Masaki Hagihara
  • Organizer: The 4th HiPep-Okinawa International Workshop Program
  • Place of Presentation: 沖縄産業支援センター(招待講演)
  • Year and Date: 2010-07-09
• [Presentation] Antisense-induced guanine quadruplexes interfere with reverse transcription by HIV-1 reverse transcriptase (2010)
  • Author(s): Masaki Hagihara, Lisa Yamauchi, Akiko Seo, Keisuke Yoneda, Mayo Senda, Kazuhiko Nakatani
  • Organizer: 第37回国際核酸化学シンポジウム
  • Place of Presentation: 横浜・みなとみらい
• [Remarks]
  • URL: http://www.sanken.osaka-u.ac.jp/labs/rbc/member/hagihara.html