Development of the affinity matrix for comprehensive analysis of ligand- binding proteins

• Project/Area Number: 22710219
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Living organism molecular science
• Research Institution: Kyoto Prefectural University
• Principal Investigator: KURAMOCHI Kouji 京都府立大学, 大学院・生命環境科学研究科, 准教授 (90408708)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000); Fiscal Year 2012: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2011: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: ケミカルバイオロジー / アフィニティー精製 / ファージディスプレイ / 固相合成用樹脂 / マンノース / コンカナバリンA / 生体分子 / 生理活性 / 蛋白質 / 有機化学

Research Abstract

Bioactive compounds such as drugs and toxins generally show their activities by interacting with their specific receptors. In this study, we explored the binding proteins of perfluorooctanesulfonic acid, which is a man-made fluorosurfactant and global pollutant, and apigenin, which is a natural product belonging to the flavone. We also developed a novel affinity matrix for purifying the binding proteins of bioactive compounds.

Research Products

• [Journal Article] Molecular basis for the action of a dietary flavonoid revealed by the comprehensive identification of apigenin human targets (2013)
  • Author(s): Daniel Arango, Kengo Morohashi, Alper Yilmaz, Kouji Kuramochi, Arti Parihar, Bledi Brahimaj, Erich Grotewold, and Andrea I. DoseffDaniel Arango, Kengo Morohashi, Alper Yilmaz, Kouji Kuramochi, Arti Parihar, Bledi Brahimaj, Erich Grotewold, and Andrea I. Doseff
  • Journal Title: Proceedings of the National Academy of Sciences of the United States of America Volume: 110 Issue: 24 Pages: 2153-2162
  • DOI: 10.1073/pnas.1303726110
  • Peer Reviewed
• [Journal Article] Synthetic and Structure-Activity Relationship Studies on Bioactive Natural Products (2013)
  • Author(s): Kouji Kuramochi
  • Journal Title: Bioscience, Biotechnology, and Biochemistry Volume: 77 Issue: 3 Pages: 446-454
  • DOI: 10.1271/bbb.120884
  • NAID: 10031164761
  • ISSN: 0916-8451, 1347-6947
  • Peer Reviewed
• [Journal Article] Molecular basis for the action of a dietary flavonoid revealed by the comprehensive identification of apigenin human targets (2013)
  • Author(s): Daniel Arango
  • Journal Title: Proceedings of the National Academy of Sciences of the United States of America Volume: 110
  • Peer Reviewed
• [Journal Article] The antitumor agent doxorubicin binds to Fanconi anemia group F protein (2012)
  • Author(s): Kusavanagi T, Tsukuda S, Shimura S, Manita D, Iwakiri K, Kamisuki S, Takakusagi Y, Takeuchi T, Kuramochi K, Nakazaki A, Sakaguchi K, Kobayashi S, Sugawara F
  • Journal Title: Bioorg. Med. Chem. Volume: 20 Issue: 21 Pages: 6248-6255
  • DOI: 10.1016/j.bmc.2012.09.015
  • Peer Reviewed
• [Journal Article] Exploration of the binding proteins of perfluorooctane sulfonate by a T7 phage display screen (2012)
  • Author(s): Miyano Y, Tsukuda S, Sakimoto I, Takeuchi R, Shimura S, Takahashi N, Kusayanagi T, Takakusagi Y, Okado M, Matsumoto Y, Takakusagi K, Takeuchi T, Kamisuki S, Nakazaki A, Ohta K, Miura M, Kuramochi K, Mizushina Y, Kobayashi S, Sugawara F, Sakaguchi K.
  • Journal Title: Bioorg. Med. Chem. Volume: 20 Issue: 13 Pages: 3985-3990
  • DOI: 10.1016/j.bmc.2012.05.016
  • Peer Reviewed
• [Journal Article] Exploration of the binding proteins of perfluorooctane sulfonate by a T7 phage display screen (2012)
  • Author(s): Yuka Miyano
  • Journal Title: Bioorganic & Medicinal Chemistry Volume: 20(掲載予定)
  • Peer Reviewed
• [Journal Article] A screening of a library of T7 phage-displayed peptide identifies E2F-4 as an etoposide-binding protein (2011)
  • Author(s): 鷹見美穂子、高草木洋一、、太田慶介、小林進、坂口謙吾、菅原二三男
  • Journal Title: Molecules Volume: 16 Issue: 5 Pages: 4278-4294
  • DOI: 10.3390/molecules16054278
  • Peer Reviewed
• [Journal Article] Cyclosporin A Associated Helicase-Like Protein Facilitates the Association of Hepatitis C Virus RNA Polymerase with Its Cellular Cyclophilin B (2011)
  • Author(s): 諸橋謙吾、佐原広益、小林進、坂口謙吾、菅原二三男
  • Journal Title: PLoS ONE Volume: 6 Issue: 4 Pages: 18285-18285
  • DOI: 10.1371/journal.pone.0018285
  • Peer Reviewed
• [Journal Article] Cyclosporin A associated helicase-like protein facilitates the association of hepatitis C virus RNA polymerase with its cellular cyclophilin B. (2011)
  • Author(s): Kengo Morohashi
  • Journal Title: PLoS ONE Volume: 6
  • Peer Reviewed
• [Presentation] 固相合成とファージディスプレイ法を組み合わせた PFOS 結合タンパク質の探索 (2012)
  • Author(s): 宮野 友香、九十田 千子、崎元 一平、武内 亮、 志村 聡美、 高橋 徳行、 草柳 友恵、高草木 洋一、岡戸 真実、松本 勇記、高草木 香織、 竹内 倫文、 紙透 伸治、 中崎 敦夫、太田 慶祐、三浦 雅彦、倉持 幸司、水品 善之、 小林 進、 菅原 二三男、 坂口 謙吾
  • Organizer: 日本農芸化学会関西支部大会、日本農芸化学会関西支部大会
  • Place of Presentation: 京都学園大学(京都府)
  • Year and Date: 2012-09-29
• [Presentation] 生物活性の探索と解明を指向した有用化合物の合成研究と化学生物学的研究 (2012)
  • Author(s): 倉持 幸司
  • Organizer: 日本農芸化学会 関西支部 第474回講演会
  • Place of Presentation: 京都府立大学(京都府);招待講演
  • Year and Date: 2012-05-26
• [Presentation] 生物活性の探索と解明を指向した有用化合物の合成研究と化学生物学的研究 (2012)
  • Author(s): 倉持 幸司
  • Organizer: 日本農芸化学会 2012 年度大会
  • Place of Presentation: ウェスティン都ホテル京都(京都府);招待講演
  • Year and Date: 2012-03-22
• [Presentation] 生物活性の探索と解明を指向した有用化合物の合成研究と化学生物学的研究 (2012)
  • Author(s): 倉持幸司
  • Organizer: 日本農芸化学会農芸化学奨励賞受賞講演
  • Place of Presentation: ウェスティン都ホテル京都(招待講演)
  • Year and Date: 2012-03-22
• [Presentation] 光固定化とファージディスプレイ法を融合した薬剤結合タンパク質の探索 (2011)
  • Author(s): 倉持 幸司
  • Organizer: 生物化学的測定法研究会、第16回学術シンポジウム
  • Place of Presentation: 京都高度技術研究所(京都府);招待講演
  • Year and Date: 2011-11-02
• [Presentation] 光固定化とファージディスプレイ法を融合した薬剤結合タンパク質の探索 (2011)
  • Author(s): 倉持幸司
  • Organizer: 生物化学的測定法研究会、第16回学術シンポジウム
  • Place of Presentation: 京都高度技術研究所(招待講演)
  • Year and Date: 2011-11-02
• [Presentation] Innovative approach to identify cellular targets of the anti-inflammatory flavonoid apigenin (2011)
  • Author(s): Daniel Arango
  • Organizer: The T2C Comprehensive Wound Care Conference
  • Place of Presentation: Ohio State University
  • Year and Date: 2011-04-08
• [Presentation] 生物活性の探索と解明を指向した有用化合物の合成研究と化学生物学的研究
  • Author(s): 倉持 幸司
  • Organizer: 日本農芸化学会関西支部 第474回 講演会
  • Place of Presentation: 京都府立大学
  • Invited
• [Presentation] 固相合成とファージディスプレイ法を組み合わせたPFOS結合タンパク質の探索
  • Author(s): 倉持 幸司
  • Organizer: 日本農芸化学会関西支部大会
  • Place of Presentation: 京都学園大学