| Budget Amount *help |
¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2012: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2011: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
|
|---|
| Research Abstract |
The analysis of the biological mechanism of small bioactive molecules in cells contributes to not only the basic research but also drug discovery research. For analyzing the mechanism of bioactive molecules, one of their approaches is to detect the direct interaction between small molecules and its target protein. Although this approach is quite effective and conduced breakthrough results, success probability is still not enough. In this research, in addition to the existing technique, it aims to provide one of the alternative methods for the direct detection technique. In detail, after preparing the cells incorporated the photoreactive amino acids in their protein and bioactive molecules attached to detecting moiety, we mixed these materials and induced covalent cross-linking between bioactive molecule and its target protein by radiating ultraviolet. Finally this approach appeared to improve the detection sensitivity.
|
