Development and application of fluorine-based photoaffinity probe

• Project/Area Number: 22710229
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Living organism molecular science
• Research Institution: Tokyo University of Science (2011-2012); The Institute of Physical and Chemical Research (2010)
• Principal Investigator: KAMISUKI Shinji 東京理科大学, 理工学部, 助教 (30553846)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: 光親和性標識 / 標的タンパク質 / 標的タンパク質同定

Research Abstract

In this study, we have demonstrated development of novel photoaffinity labeling, in which 4-fluoroindole was utilized as a photoreactive group. Model study revealed that Neutravidin photolabeled with biotinylated 4-fluorotryptophan could be detected. This method is expected to be applied for identification of receptor proteins for peptide ligand

Research Products

• [Journal Article] Ridaifen B, a tamoxifen derivative, directly binds to Grb10 interacting GYF protein 2 (2013)
  • Author(s): 九十田千子
  • Journal Title: ioorgnic & Medicinal Chemistry Volume: 21巻 Issue: 1 Pages: 311-320
  • DOI: 10.1016/j.bmc.2012.10.037
  • Peer Reviewed
• [Journal Article] Exploration of the binding proteins of perfluorooctane sulfonate by a T7 phage display screen (2012)
  • Author(s): Miyano Y, Tsukuda S, Sakimoto I, Takeuchi R, Shimura S, Takahashi N, Kusayanagi T, Takakusagi Y, Okado M, Matsumoto Y, Takakusagi K, Takeuchi T, Kamisuki S, Nakazaki A, Ohta K, Miura M, Kuramochi K, Mizushina Y, Kobayashi S, Sugawara F, Sakaguchi K.
  • Journal Title: Bioorg. Med. Chem. Volume: 20 Issue: 13 Pages: 3985-3990
  • DOI: 10.1016/j.bmc.2012.05.016
  • Peer Reviewed
• [Journal Article] Total Synthesis of (+)-Sch 725680: Inhibitor of Mammalian A-, B-, and Y-Family DNA Polymerases (2012)
  • Author(s): 竹内倫文
  • Journal Title: Organic Letters Volume: 14巻 Issue: 17 Pages: 4303-4305
  • DOI: 10.1021/ol301865u
  • Peer Reviewed
• [Journal Article] The antitumor agent doxorubicin binds to Fanconi anemia group F protein (2012)
  • Author(s): Kusavanagi T, Tsukuda S, Shimura S, Manita D, Iwakiri K, Kamisuki S, Takakusagi Y, Takeuchi T, Kuramochi K, Nakazaki A, Sakaguchi K, Kobayashi S, Sugawara F
  • Journal Title: Bioorg. Med. Chem. Volume: 20 Issue: 21 Pages: 6248-6255
  • DOI: 10.1016/j.bmc.2012.09.015
  • Peer Reviewed
• [Journal Article] Synthesis and Evaluation of Diarylthiazole Derivatives That Inhibit Activation of Sterol Regulatory Element-Binding Proteins (2011)
  • Author(s): 紙透伸治
  • Journal Title: Journal of Medicinal Chemistry Volume: 54巻 Issue: 13 Pages: 4923-4927
  • DOI: 10.1021/jm200304y
  • Peer Reviewed
• [Journal Article] Catch and release of alkyne-tagged molecules in water by a polymer-supported cobalt complex (2011)
  • Author(s): 江上寛通
  • Journal Title: Organic & Biomolecular Chemistry Volume: 9巻 Issue: 22 Pages: 7667-7670
  • DOI: 10.1039/c1ob06123b
  • Peer Reviewed
• [Journal Article] Camptothecin (CPT) directly binds to human heterogeneous nuclear ribonucleoprotein A1 (hnRNP A1) and inhibits the hnRNP A1/ topoisomerase I interaction (2011)
  • Author(s): 真仁田大輔
  • Journal Title: Bioorgnic & Medicinal Chemistry Volume: 19巻 Issue: 24 Pages: 7690-7697
  • DOI: 10.1016/j.bmc.2011.09.059
  • Peer Reviewed
• [Presentation] 脂肪合成を阻害する小分子化合物 (2011)
  • Author(s): 紙透伸治
  • Organizer: 日本植物学会
  • Place of Presentation: 名古屋
  • Year and Date: 2011-09-09