Metabolite profiling in rice plant by Multidimensional NMR-Based Metabolomics

• Project/Area Number: 22710230
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Living organism molecular science
• Research Institution: National Agriculture and Food Research Organization
• Principal Investigator: SEKIYAMA Yasuyo 独立行政法人農業・食品産業技術総合研究機構, 食品総合研究所・食品分析研究領域, 任期付研究員 (60342804)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000); Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2010: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 機器分析 / メタボローム解析 / 核磁気共鳴法 / 安定同位体標識 / メタボローム / イネ

Research Abstract

^<13> C labeling allows to increase the reliability of metabolite annotation. In an attempt to expand information on metabolite profiles in Oryza sativa, I tried to generate highly^<13> C-labeled rice plants by^<13> CO_2 feeding. The labeling ratio of the labeled plant estimated from the anomeric proton signal of sucrose in the 1^H NMR spectra was 75%. Candidate polar and semipolar metabolites in the leaves were listed from the standard database.

Research Products

• [Journal Article] NMR計測による生体代謝物情報の抽出 (2012)
  • Author(s): 関山恭代、近山英輔、菊地淳
  • Journal Title: ぶんせき Volume: 2月号 Pages: 81-88
  • NAID: 10030723663
• [Journal Article] Evaluation of a Semipolar Solvent System as a Step toward Heteronuclear Multidimensional NMR-Based Metabolomics for C^<13>-Labeled Bacteria (2011)
  • Author(s): Yasuyo Sekiyama, Eisuke Chikayama, Jun Kikuchi
  • Journal Title: Plants, and Animals, Analytical Chemistry Volume: 83 Pages: 719-726
  • Peer Reviewed
• [Presentation] NMR法によるメタボローム解析の現状と農作物解析への応用 (2012)
  • Author(s): 関山恭代
  • Organizer: 総合研究試験研究推進会議土壌肥料研究会
  • Place of Presentation: つくば
  • Year and Date: 2012-02-21
• [Presentation] NMR法によるメタボローム解析の現状と農作物解析への応用 (2012)
  • Author(s): 関山恭代
  • Organizer: 総合研究試験研究推進会議土壌肥料研究会第1分科会「メタボローム解析技術の農業への展開」
  • Place of Presentation: 農林水産技術会議筑波事務所(茨城県)(招待講演)
  • Year and Date: 2012-02-21
• [Presentation] NMR-based metabolite profiling in potato and rice plants (2012)
  • Author(s): Yasuyo Sekiyama, Seishi Ikeda, Shogo Tsuda, Nobutaka Someya, Eisuke Chikayama, Jun Kikuchi, Hiroshi Ono
  • Organizer: 8th Annual International Meeting of the Metabolomics Society
  • Place of Presentation: Washington D. C