| Project/Area Number |
22710234
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Resource conservation science
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| Research Institution | University of Yamanashi |
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Principal Investigator |
YAMAMURA Hideki 山梨大学, 大学院・医学工学総合研究部, 助教 (70516939)
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| Project Period (FY) |
2010 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2011: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2010: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
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| Keywords | 微生物 / 保全 / 放線菌 / 分類学 / 生態学 / 多様性 / 遺伝資源 / 亜熱帯 / ミコール酸含有菌 / 系統解析 / 選択分離法 / 16S rRNA遺伝子 / 微生物多様性 / 抗生物質耐性 |
|---|
| Research Abstract |
The aim of this study was to reveal the diversity and conservation of mycolic acid containing actinomycete such as Nocardia and Mycobacterium from subtropical area in Japan. Forest and cultivated field soil samples were collected from Iriomote island, Okinawa, Japan. The isolates belonging to the genus Nocardia and Mycobacterium were selectively isolated using sucrose-gradient centrifugation method and Oil-SDS emulsification method. Twelve species of Nocardia and two species of Mycobacterium was detected. Selective isolation method for the genus Rhodococcus was developed as combined use of antibiotics(fradiomycine ; 20 mg/L and novobiocin ; 10 mg/L) and pretreatment of soil with alkaline-SDS solution. Some of isolates were suspected as new species and were deposited in NITE-Biological Resource Center for microbial conservation
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