Construction of the technical bases for conservation genetics in Aegagropila linnaei in Lake Akan

• Project/Area Number: 22710239
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Resource conservation science
• Research Institution: University of Tsukuba (2011-2012); National Institute for Environmental Studies (2010)
• Principal Investigator: NISHIZAWA Toru 筑波大学, 生命環境系, 研究員 (80414382)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2012: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: マリモ / 阿寒湖 / 分子マーカー / マイクロサテライト / 遺伝的多様性 / 個体群保全 / 個体群復元

Research Abstract

I developed microsatellite markers in Aegagropia linnaei, a green algae that distributes in Lake Akan. However, polymorphic loci were not captured in this study duration. I also considered optimum conditions which allow the nuclear-phase analysis that covers a single fragment specimen. Due to extremely low DNA content in a single fragment specimen, the nuclear-phase analysis using flow cytometry was impossible.