Microirradiation system for centrosome in human-derived living cells
| Project/Area Number |
22740269
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Atomic/Molecular/Quantum electronics
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| Research Institution | The Institute of Physical and Chemical Research |
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Principal Investigator |
MEISSL WALTER 独立行政法人理化学研究所, 山崎原子物理研究室, 訪問研究員 (30548970)
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| Project Period (FY) |
2010 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2011: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2010: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
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| Keywords | ビーム物理 / マイクロビーム / ガラスキャピラリー / 生物物理 |
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| Research Abstract |
A fully automatic 3-D target stage for the microirradiation of living cells was successfully acquired. We can thus visit any cell within the target zone of a cell dish and position target any point within the cell precisely. We could, for example, add an algorithm to identify cells automatically or integrate the ability to get so called z-stack images which allow a 3-dimensional representation of cells and areas affected by irradiation. First tests of the system show that all components are fully working and that the desired sub-micrometer precision is reached in all three dimensions. Living human cells kept in the on-stage incubator for several days remained healthy over the full period and the cell cycle of the cells was measured as less than 20 hours. In a first test with fixed(not moving) cells we were able to precisely aim the ion beam at the circa 1 micrometer wide centrosome, an organelle within the cells. The improved system will allow precise targeting of sub-cellular components as well as tracking of irradiated cells for several days without any harmful effects on the cells.
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Report
(3 results)
Research Products
(14 results)
