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Development of the techniques for the protein patterning by atmospheric pressure plasmas

Research Project

Project/Area Number 22740365
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Plasma science
Research InstitutionOsaka University

Principal Investigator

ANDO Ayumi  大阪大学, 大学院・工学研究科, 特任研究員 (60452471)

Project Period (FY) 2010 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2010: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywordsプラズマ / 大気圧プラズマ / 細胞外マトリックス / マイクロパターニング / バイオチップ / ラジカル / パターニング
Research Abstract

Extracellular matrices(ECMs) patterns of many tens or hundreds micrometers in size have been formed on silicon substrates with the use of low-temperature atmospheric-pressure plasma(APP) jets with metal stencil masks in this research. Neuronal model cells were shown to grow on the ECM patterned by APP jets, so that the patterning technique by low-temperature APPs for ECM layers provides a simple mean of arranging neuron cells on a relatively large area of a silicon surface.

Report

(3 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report
  • Research Products

    (22 results)

All 2012 2011 2010

All Journal Article (8 results) (of which Peer Reviewed: 6 results) Presentation (14 results)

  • [Journal Article] Micro-patterning formation of extracellular-matrix(ECM) layers2012

    • Author(s)
      Ayumi Ando, Toshifumi Asano, Tsuneo Urisu and Satoshi Hamaguchi
    • Journal Title

      Europhysics news

      Volume: 43(3) Pages: 13-13

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Extracellular Matrix Patterning for Cell Alignment by Atmospheric Pressure Plasma Jets2012

    • Author(s)
      Ayumi Ando, Toshifumi Asano, Md. Abu Sayed, Ryugo Tero, Katsuhisa Kitano, Tsuneo Urisu and Satoshi Hamaguchi
    • Journal Title

      Japanese Journal of Applied Physics

      Volume: 51 Issue: 3R Pages: 36201-36201

    • DOI

      10.1143/jjap.51.036201

    • NAID

      40019199845

    • Related Report
      2011 Annual Research Report 2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Arrangement of PC12 Cells on a Silicon Chip via Extracellular Matrix (ECM) Layer Patterning by Atmospheric Pressure Plasmas2011

    • Author(s)
      Ayumi Ando, Hidetaka Uno, Toshifumi Asano, Tsuneo Urisu and Satoshi Hamaguchi
    • Journal Title

      Plasma and Fusion Research

      Volume: 6 Pages: 1306155-1306155

    • DOI

      10.1585/pfr.6.1306155

    • NAID

      130002059489

    • ISSN
      1880-6821
    • Related Report
      2011 Annual Research Report 2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Micro pattern formation of extracellular-matrix(ECM) layers by atmospheric-pressure plasmas and cell culture on the patterned ECMs2011

    • Author(s)
      Ayumi Ando, Toshifumi Asano, Tsuneo Urisu and Satoshi Hamaguchi
    • Journal Title

      Journal of Physics D : Applied Physics

      Volume: 44 Issue: 48 Pages: 482002-482002

    • DOI

      10.1088/0022-3727/44/48/482002

    • Related Report
      2011 Annual Research Report 2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Patterning of an extracellular matrix(ECM) layer on silicon by atmospheric-pressure plasmas for living neuron network chip fabrication2011

    • Author(s)
      Ayumi Ando, Toshifumi Asano, Hidetaka Uno, Ryugo Tero, Katsuhisa Kitano, Tsuneo Urisu and Satoshi Hamaguchi
    • Journal Title

      Proceedings of the 20th International Symposium on Plasma Chemistry(ISPC 20)

      Volume: Paper No.MED07

    • Related Report
      2011 Final Research Report
  • [Journal Article] Protein pattering by atmospheric-pressure plasmas2010

    • Author(s)
      Ayumi Ando, Md. Abu Sayed, Toshifumi Asano, Ryugo Tero, Katsuhisa Kitano, Tsuneo Urisu and Satoshi Hamaguchi
    • Journal Title

      Proceedings of 4^<th> International Symposium on Atomic Technology(ISAT-4), Journal of Physics ; Conference Series

      Volume: No.232 Pages: 12019-12019

    • DOI

      10.1088/1742-6596/232/1/012019

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Protein pattering by atmospheric-pressure plasmas2010

    • Author(s)
      安藤あゆみ
    • Journal Title

      Proceedings of 4^<th> International Symposium on Atomic Technology (ISAT-4), Journal of Physics ; Conference Series

      Volume: No.232

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Extracellular matrix patterning by atmospheric pressure plasma jets2010

    • Author(s)
      安藤あゆみ
    • Journal Title

      Proceedings of 7^<th> International Conference on Reactive Plasmas, 28th Symposium on Plasma Processing and 63rd Gaseous Electronics Conference (ICRP-7/SPP-28/GEC-63)

    • Related Report
      2010 Annual Research Report
  • [Presentation] Arrangement of PC12 cells on extracellular matrix(ECM) layers patterned by atmospheric pressure plasmas2012

    • Author(s)
      Ayumi Ando, Hidetaka Uno, Tsuneo Urisu and Satoshi Hamaguchi
    • Organizer
      The 8^<th> EU-Japan Joint Symposium on Plasma Processing(JSPP2012)
    • Place of Presentation
      Todaiji Culture Center, Todaiji Temple, Nara, Japan, Book of abstracts P33
    • Year and Date
      2012-01-17
    • Related Report
      2011 Final Research Report
  • [Presentation] Arrangement of PC12 cells on extracellular matrix (ECM) layers patterned by at mospheric pressure plasmas2012

    • Author(s)
      Ayumi Ando
    • Organizer
      The 8^<th> EU-Japan Joint Symposium on Plasma Processing (JSPP2012)
    • Place of Presentation
      Nara, Japan
    • Year and Date
      2012-01-17
    • Related Report
      2011 Annual Research Report
  • [Presentation] Orthopaedic Applications of Low-temperature plasmas2012

    • Author(s)
      Kazuto Masuda
    • Organizer
      The 8^<th> EU-Japan Joint Symposium on Plasma Processing (JSPP2012)
    • Place of Presentation
      Nara, Japan
    • Year and Date
      2012-01-17
    • Related Report
      2011 Annual Research Report
  • [Presentation] Extracellular matrix(ECM) patterning by atmospheric-pressure plasmas for arranging neuronal cells on silicon substrates2011

    • Author(s)
      Ayumi Ando, Toshifumi Asano, Hidetaka Uno, Ryugo Tero, Katsuhisa Kitano, Tsuneo Urisu and Satoshi Hamaguchi
    • Organizer
      Plasma Conference 2011(PLASMA 2011)
    • Place of Presentation
      Ishikawa Ongakudo, Kanazawa, Japan, 25B05.(招待講演)
    • Year and Date
      2011-11-25
    • Related Report
      2011 Final Research Report
  • [Presentation] Extracellular matrix (ECM) patterning by atmospheric-pressure plasmas for arranging neuronal cells on silicon substrates2011

    • Author(s)
      Ayumi Ando
    • Organizer
      Plasma Conference 2011 (PLASMA 2011)
    • Place of Presentation
      Kanazawa, Japan(招待講演)
    • Year and Date
      2011-11-25
    • Related Report
      2011 Annual Research Report
  • [Presentation] Patterning of an extracellular matrix(ECM) layer on silicon by atmospheric-pressure plasmas for living neuron network chip fabrication2011

    • Author(s)
      Ayumi Ando, Toshifumi Asano, Hidetaka Uno, Ryugo Tero, Katsuhisa Kitano, Tsuneo Urisu and Satoshi Hamaguchi
    • Organizer
      The 20th International Symposium on Plasma Chemistry(ISPC 20)
    • Place of Presentation
      Loews Hotel, Philadelphia, USA, MED07
    • Year and Date
      2011-07-28
    • Related Report
      2011 Final Research Report
  • [Presentation] Patterning of an extracellular matrix (ECM) layer on silicon by atmospheric-pressure plasmas for living neuron network chip fabrication2011

    • Author(s)
      Ayumi Ando
    • Organizer
      The 20th International Symposium on Plasma Chemistry (ISPC 20)
    • Place of Presentation
      Philadelphia, USA
    • Year and Date
      2011-07-28
    • Related Report
      2011 Annual Research Report
  • [Presentation] Plasma Irradiation Effects on Cells, Tissues, and Biomaterials in Orthopaedic Applications2011

    • Author(s)
      Kazuto Masuda
    • Organizer
      The 20th International Symposium on Plasma Chemistry (ISPC 20)
    • Place of Presentation
      Philadelphia, USA
    • Year and Date
      2011-07-28
    • Related Report
      2011 Annual Research Report
  • [Presentation] 大気圧低温プラズマによる神経細胞ネットワーク用細胞外マトリックスパターニング技術の開発2011

    • Author(s)
      安藤あゆみ
    • Organizer
      春季第58回応用物理学関係連合講演会
    • Place of Presentation
      神奈川工科大学(神奈川県)
    • Year and Date
      2011-03-26
    • Related Report
      2010 Annual Research Report
  • [Presentation] 大気圧低温プラズマによる神経細胞ネットワーク用細胞外マトリックスパターニング技術の開発2011

    • Author(s)
      安藤あゆみ, 宇野秀隆, 浅野豪文, 手老龍吾, 北野勝久, 宇理須恒雄, 浜口智志
    • Organizer
      第58回応用物理学関係連合講演会,第58回応用物理学関係連合講演会予稿集
    • Related Report
      2011 Final Research Report
  • [Presentation] 大気圧プラズマによる神経細胞ネットワークチップ用細胞外マトリックスパターニング2010

    • Author(s)
      安藤あゆみ, 浅野豪文, 手老龍吾, 北野勝久, 宇理須恒雄, 浜口智志
    • Organizer
      第27回プラズマ・核融合学会年会
    • Place of Presentation
      北海道大学
    • Year and Date
      2010-12-01
    • Related Report
      2011 Final Research Report
  • [Presentation] 大気圧プラズマによる神経細胞ネットワークチップ用細胞外マトリックスパターニング2010

    • Author(s)
      安藤あゆみ
    • Organizer
      第27回プラズマ・核融合学会年会
    • Place of Presentation
      北海道大学(北海道)
    • Year and Date
      2010-12-01
    • Related Report
      2010 Annual Research Report
  • [Presentation] Extracellular matrix patterning by atmospheric pressure plasma jets2010

    • Author(s)
      安藤あゆみ
    • Organizer
      7^<th> International Conference on Reactive Plasmas, 28th Symposium on Plasma Processing and 63rd Gaseous Electronics Conference (ICRP-7/SPP-28/GEC-63)
    • Place of Presentation
      Maison de la chimie (Paris, France)
    • Year and Date
      2010-10-06
    • Related Report
      2010 Annual Research Report
  • [Presentation] Extracellular matrix patterning by atmospheric pressure plasma jets2010

    • Author(s)
      Ayumi Ando, Toshifumi Asano, Ryugo Tero, Katsuhisa Kitano, Tsuneo Urisu and Satoshi Hamaguchi
    • Organizer
      7^<th> International Conference on Reactive Plasmas, 28th Symposium on Plasma Processing and 63rd Gaseous Electronics Conference(ICRP-7/ SPP-28/ GEC-63)
    • Place of Presentation
      Maison de la chimie, Paris, France, KWP. 00034
    • Related Report
      2011 Final Research Report

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Published: 2010-08-23   Modified: 2016-04-21  

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