Development of techniques for single-molecule photon statistics and real-time measurements toward elucidation of folding processes in protein molecules
| Project/Area Number |
22750013
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Physical chemistry
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| Research Institution | National Institute of Information and Communications Technology |
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Principal Investigator |
KAJI Takahiro 独立行政法人情報通信研究機構, 未来ICT 研究所 ナノICT 研究室, 研究員 (40573134)
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| Project Period (FY) |
2010 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2012: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2011: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2010: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
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| Keywords | 蛋白質 / 生物物理 / 1 分子計測(SMD) / 共焦点顕微鏡 / １分子計測（SMD） / フォトニック結晶 / 1分子計測(SMD) |
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| Research Abstract |
We succeeded in preparing two-dimensional photonic crystal slabs that have low background emission in visible light region and observing fluorescence enhancement of single molecules on the photonic crystals. In addition, we found that the fluorescence lifetime varies greatly depending on the orientation of the fluorescent dye using a two-dimensional photonic band gap. From the results, we showed a new way to increase the sensitivity of single-molecule fluorescence spectroscopy as well as to determine the orientation of the fluorescent dye by analyzing change in the fluorescence lifetime using photonic crystals that have nanospaces needed for local spectroscopic measurements of single biomolecules.
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Report
(4 results)
Research Products
(42 results)
