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Development of techniques for single-molecule photon statistics and real-time measurements toward elucidation of folding processes in protein molecules

Research Project

Project/Area Number 22750013
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Physical chemistry
Research InstitutionNational Institute of Information and Communications Technology

Principal Investigator

KAJI Takahiro  独立行政法人情報通信研究機構, 未来ICT 研究所 ナノICT 研究室, 研究員 (40573134)

Project Period (FY) 2010 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2012: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2011: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2010: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Keywords蛋白質 / 生物物理 / 1 分子計測(SMD) / 共焦点顕微鏡 / 1分子計測(SMD) / フォトニック結晶 / 1分子計測(SMD)
Research Abstract

We succeeded in preparing two-dimensional photonic crystal slabs that have low background emission in visible light region and observing fluorescence enhancement of single molecules on the photonic crystals. In addition, we found that the fluorescence lifetime varies greatly depending on the orientation of the fluorescent dye using a two-dimensional photonic band gap. From the results, we showed a new way to increase the sensitivity of single-molecule fluorescence spectroscopy as well as to determine the orientation of the fluorescent dye by analyzing change in the fluorescence lifetime using photonic crystals that have nanospaces needed for local spectroscopic measurements of single biomolecules.

Report

(4 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Annual Research Report
  • 2010 Annual Research Report
  • Research Products

    (42 results)

All 2013 2012 2011 2010 Other

All Journal Article (8 results) (of which Peer Reviewed: 6 results) Presentation (28 results) Remarks (4 results) Patent(Industrial Property Rights) (2 results)

  • [Journal Article] Controlled Spontaneous Emission of Single Molecules in a Two-Dimensional Photonic Band Gap2012

    • Author(s)
      Takahiro Kaji
    • Journal Title

      Journal of the American Chemical Society

      Volume: 135 Issue: 1 Pages: 106-109

    • DOI

      10.1021/ja3115357

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Orientation of a bacteriorhodopsin thin film deposited by dip coating technique and its chiral SHG as studied by SHG interference technique2012

    • Author(s)
      Toshiki Yamada、Yoshihiro Haruyama、 Katsuyuki Kasai 、Toshifumi Terui 、 Shukichi Tanaka 、Takahiro Kaji 、 Hiroshi Kikuchi 、Akira Otomo
    • Journal Title

      Chemical Physics Letters

      Volume: Vol.530 Pages: 113-119

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Orientation of a bacteriorhodopsin thin film deposited by dip coating technique and its chiral SHG as studied by SHG interference technique2012

    • Author(s)
      Toshiki Yamada
    • Journal Title

      Chemical Physics Letters

      Volume: 530 Pages: 113-119

    • DOI

      10.1016/j.cplett.2012.01.063

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Enhanced fluorescence emission from single molecules on a two-dimensional photonic crystal slab with low background emission2011

    • Author(s)
      Takahiro Kaji、Toshiki Yamada、Rieko Ueda 、Akira Otomo
    • Journal Title

      The Journal of Physical Chemistry Letters

      Volume: Vol.2 Issue: 14 Pages: 1651-1656

    • DOI

      10.1021/jz2006989

    • Related Report
      2012 Final Research Report 2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Fabrication of two-dimensional Ta2O5 photonic crystal slabs with ultra-low background emission toward highly sensitive fluorescence spectroscopy2011

    • Author(s)
      Takahiro Kaji、Toshiki Yamada、Rieko Ueda 、Xingsheng Xu 、Akira Otomo
    • Journal Title

      Optics Express

      Volume: Vol.19 Issue: 2 Pages: 1422-1428

    • DOI

      10.1364/oe.19.001422

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] 新規な顕微鏡集光ユニット及び時間相 関単一光子計数系の開発と応用―様々 な周囲環境下及び電磁場環境下におけ る単一分子分光―2011

    • Author(s)
      山田俊樹、梶 貴博、上田里永子、大友 明
    • Journal Title

      信学技報(社団法人電子情報通信学会)

      Volume: Vol.111、No.4 Pages: 31-36

    • URL

      http://iss.ndl.go.jp/books/R000000004-I11069494-00

    • Related Report
      2012 Final Research Report
  • [Journal Article] Fabrication of two-dimensional Ta_2O_5 photonic crystal slabs with ultra-low background emission toward highly sensitive fluorescence spectroscopy2011

    • Author(s)
      Takahiro Kaji
    • Journal Title

      OPTICS EXPRESS

      Volume: 19 Pages: 1422-1428

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 新規な顕微鏡集光ユニット及び時間相関単一光子計数系の開発と応用-様々な周囲環境下及び電磁場環境下における単一分子分光-2011

    • Author(s)
      山田俊樹
    • Journal Title

      信学技報(社団法人電子情報通信学会)

      Volume: vol.111 No.4 Pages: 31-36

    • Related Report
      2010 Annual Research Report
  • [Presentation] 単一分子の発光制御を実現する2 次元フォトニック結晶プラットフォームの開発2013

    • Author(s)
      梶 貴博、山田俊樹、伊都将司、宮坂 博、上田里永子、井上振一郎、大友 明
    • Organizer
      2013 年第60回応用物理学会春季学術講演会
    • Place of Presentation
      神奈川工科大学、神奈川県
    • Related Report
      2012 Final Research Report
  • [Presentation] 2 次元フォトニックバンドギャップ中の単一分子蛍光挙動の統計的解析2013

    • Author(s)
      梶 貴博、山田俊樹、上田里永子、井上振一郎、大友 明
    • Organizer
      日本化学会第93 春季年会(2013)
    • Place of Presentation
      立命館大学 びわこ・くさつキャンパス、滋賀県
    • Related Report
      2012 Final Research Report
  • [Presentation] Control of spontaneous emission from single molecules in a photonic band gap of a two-dimensional photonic crystal slab2013

    • Author(s)
      Takahiro Kaji、Toshiki Yamada、Syoji Ito、 Hiroshi Miyasaka、Rieko Ueda、 Shin-ichiro Inoue 、Akira Otomo
    • Organizer
      Seventh International Conference on Molecular Electronics and Bioelectronics (M&BE7)
    • Place of Presentation
      Fukuoka International Cogress Center 、 Fukuoka、Japan
    • Related Report
      2012 Final Research Report
  • [Presentation] Control of spontaneous emission from single molecules in a photonic band gap of a two-dimensional photonic crystal slab2013

    • Author(s)
      Takahiro Kaji
    • Organizer
      Seventh International Conference on Molecular Electronics and Bioelectronics (M&BE7)
    • Place of Presentation
      Fukuoka International Congress Center, Fukuoka, Japan
    • Related Report
      2012 Annual Research Report
  • [Presentation] 2次元フォトニックバンドギャップ中の単一分子蛍光挙動の統計的解析2013

    • Author(s)
      梶 貴博
    • Organizer
      日本化学会第93春季年会
    • Place of Presentation
      立命館大学 びわこ・くさつキャンパス (滋賀県)
    • Related Report
      2012 Annual Research Report
  • [Presentation] 単一分子の発光制御を実現する2次元フォトニック結晶プラットフォームの開発2013

    • Author(s)
      梶 貴博
    • Organizer
      第60回応用物理学会春季学術講演会
    • Place of Presentation
      神奈川工科大学 (神奈川県)
    • Related Report
      2012 Annual Research Report
  • [Presentation] 2次元フォトニック結晶スラブ上の単一分子の発光挙動2012

    • Author(s)
      梶貴博
    • Organizer
      日本化学会第92春季年会(2012)
    • Place of Presentation
      慶應義塾大学日吉キャンパス・矢上キャンパス(神奈川県)
    • Year and Date
      2012-03-27
    • Related Report
      2011 Annual Research Report
  • [Presentation] 2次元フォトニック結晶スラブを用いた高感度な単一分子蛍光測定2012

    • Author(s)
      梶貴博
    • Organizer
      2012年春季第59回応用物理学関係連合講演会
    • Place of Presentation
      早稲田大学早稲田キャンパス(東京都)
    • Year and Date
      2012-03-16
    • Related Report
      2011 Annual Research Report
  • [Presentation] Photonic crystals for enhancing fluorescence from single molecules2012

    • Author(s)
      Takahiro Kaji、Toshiki Yamada、Rieko Ueda、Shin-ichiro Inoue、Akira Otomo
    • Organizer
      10th International Conference on Nano-Molecular Electronics (ICNME)
    • Place of Presentation
      Awaji Yumebutai International Conference Center、Awaji、Hyogo、Japan
    • Related Report
      2012 Final Research Report
  • [Presentation] 2次元フォトニックバンドギャップによる単一分子の自然放出抑制2012

    • Author(s)
      梶 貴博、山田俊樹、伊都将司、宮坂 博、 上田里永子、井上振一郎、大友 明
    • Organizer
      2012 年光化学討論会
    • Place of Presentation
      東京工業大学 大岡山キャンパス、東京都
    • Related Report
      2012 Final Research Report
  • [Presentation] 2 次元フォトニック結晶スラブ上の単一分子の発光挙動2012

    • Author(s)
      梶 貴博、山田俊樹、井上振一郎、上田里永子、大友 明
    • Organizer
      日本化学会第92 春季年会(2012)
    • Place of Presentation
      慶應義塾大学 日吉キャンパス・矢上キャンパス、神奈川県
    • Related Report
      2012 Final Research Report
  • [Presentation] 2 次元フォトニック結晶スラブを用いた高感度な単一分子蛍光測定2012

    • Author(s)
      梶 貴博、山田俊樹、井上振一郎、上田里永子、大友 明
    • Organizer
      2012 年春季第59回応用物理学関係連合講演会
    • Place of Presentation
      早稲田大学 早稲田キャンパス
    • Related Report
      2012 Final Research Report
  • [Presentation] Photonic crystals for enhancing fluorescence from single molecules2012

    • Author(s)
      Takahiro Kaji
    • Organizer
      10th International Conference on Nano-Molecular Electronics
    • Place of Presentation
      Awaji Yumebutai International Conference Center, Awaji, Hyogo, Japan
    • Related Report
      2012 Annual Research Report
  • [Presentation] 2次元フォトニックバンドギャップによる単一分子の自然放出抑制2012

    • Author(s)
      梶 貴博
    • Organizer
      2012年光化学討論会
    • Place of Presentation
      東京工業大学 大岡山キャンパス (東京都)
    • Related Report
      2012 Annual Research Report
  • [Presentation] Fabrication and Characterization of a Two-Dimensional Photonic Crystal Slab with Low Background Emission for Enhancing Fluorescence Emission from Single Molecules2011

    • Author(s)
      Takahiro Kaji
    • Organizer
      XXV International Conference on Photochemistry (ICP2011)
    • Place of Presentation
      Beijing Friendship Hotel, Beijing, China
    • Year and Date
      2011-10-08
    • Related Report
      2011 Annual Research Report
  • [Presentation] 低バックグラウンド発光の2次元フォトニック結晶スラブを用いた単一分子の発光制御2011

    • Author(s)
      梶貴博
    • Organizer
      2011年光化学討論会
    • Place of Presentation
      宮崎市河畔コンベンションエリア(宮崎県)
    • Year and Date
      2011-09-07
    • Related Report
      2011 Annual Research Report
  • [Presentation] 高感度蛍光顕微鏡法実現に向けた2次元Ta_2O_5 フォトニック結晶の作製2011

    • Author(s)
      梶貴博
    • Organizer
      2011年春季第58回応用物理学関係連合講演会
    • Place of Presentation
      神奈川工科大学(アブストラクト集による発表扱い)
    • Year and Date
      2011-03-27
    • Related Report
      2010 Annual Research Report
  • [Presentation] 超低バックグラウンド発光の2次元フォトニック結晶を用いたペリレンビスイミドの蛍光増強2011

    • Author(s)
      梶貴博
    • Organizer
      日本化学会第91春季年会(2011)
    • Place of Presentation
      神奈川大学(アブストラクト集による発表扱い)
    • Year and Date
      2011-03-26
    • Related Report
      2010 Annual Research Report
  • [Presentation] Enhancement of dye fluorescence using two-dimensional Ta_2O_5 photonic crystal slabs with ultra-low background emission2011

    • Author(s)
      Takahiro Kaji
    • Organizer
      Sixth International Conference on Molecular Electronics and Bioelectronics (M&BE6)
    • Place of Presentation
      Sendai International Center, Miyagi, Japan(アブストラクト集による発表扱い)
    • Year and Date
      2011-03-16
    • Related Report
      2010 Annual Research Report
  • [Presentation] 低バックグラウンド発光の2 次元フォトニック結晶スラブを用いた単一分子の発光制御2011

    • Author(s)
      梶 貴博、山田 俊樹、井上 振一郎、上田 里永子、大友 明
    • Organizer
      2011 年光化学討論会
    • Place of Presentation
      宮崎市河畔コンベンションエリア、宮崎県
    • Related Report
      2012 Final Research Report
  • [Presentation] Fabrication and characterization of a two-dimensional photonic crystal slab with low background emission for enhancing fluorescence emission from single molecules2011

    • Author(s)
      Takahiro Kaji、Toshiki Yamada、Rieko Ueda、Akira Otomo
    • Organizer
      XXV International Conference on Photochemistry (ICP2011)
    • Place of Presentation
      Beijing Friendship Hotel、Beijing、China
    • Related Report
      2012 Final Research Report
  • [Presentation] 超低バックグラウンド発光の2 次元フォトニック結晶を用いたペリレンビスイミドの蛍光増強2011

    • Author(s)
      梶 貴博、山田俊樹、上田里永子、大友 明
    • Organizer
      日本化学会第91 春季年会(2011)
    • Place of Presentation
      神奈川大学、神奈川県
    • Related Report
      2012 Final Research Report
  • [Presentation] 高感度蛍光顕微鏡法実現に向けた2 次元Ta2O5 フォトニック結晶の作製2011

    • Author(s)
      梶 貴博、山田俊樹、上田里永子、大友 明
    • Organizer
      2011年春季第58回応用物理学関係連合講演会
    • Place of Presentation
      神奈川工科大学、神奈川県
    • Related Report
      2012 Final Research Report
  • [Presentation] Enhancement of dye fluorescence using two-dimensional Ta2O5 photonic crystal slabs with ultra-low background emission2011

    • Author(s)
      Takahiro Kaji、Toshiki Yamada、Rieko Ueda、Akira Otomo
    • Organizer
      Sixth International Conference on Molecular Electronics and Bioelectronics (M&BE6)
    • Place of Presentation
      Sendai International Center、Miyagi、Japan
    • Related Report
      2012 Final Research Report
  • [Presentation] Fabrication of low background two-dimensional Ta_2O_5 photonic crystals for observing fluorescence enhancement of organic dyes2010

    • Author(s)
      Takahiro Kaji
    • Organizer
      9th International Conference on Nano-Molecular Electronics
    • Place of Presentation
      International Conference Center Kobe, Kobe, Japan
    • Year and Date
      2010-12-14
    • Related Report
      2010 Annual Research Report
  • [Presentation] 2次元フォトニック結晶上に吸着した色素分子の発光挙動2010

    • Author(s)
      梶貴博
    • Organizer
      2010年光化学討論会
    • Place of Presentation
      千葉大学(千葉県)
    • Year and Date
      2010-09-08
    • Related Report
      2010 Annual Research Report
  • [Presentation] Fabrication of low background two-dimensional Ta2O5 photonic crystals for observing fluorescence enhancement of organic dyes2010

    • Author(s)
      Takahiro Kaji、Toshiki Yamada、Rieko Ueda、Akira Otomo
    • Organizer
      9th International Conference on Nano-Molecular Electronics
    • Place of Presentation
      International Conference Center Kobe、Kobe、hyogo、Japan
    • Related Report
      2012 Final Research Report
  • [Presentation] 2 次元フォトニック結晶上に吸着した色素分子の発光挙動2010

    • Author(s)
      梶 貴博、上田里永子、山田俊樹、大友 明
    • Organizer
      2010 年光化学討論会
    • Place of Presentation
      千葉大学、千葉県
    • Related Report
      2012 Final Research Report
  • [Remarks]

    • URL

      http://www2.nict.go.jp/advanced_ict/nano/103/index.html

    • Related Report
      2012 Final Research Report
  • [Remarks] 研究成果

    • URL

      http://www2.nict.go.jp/advanced_ict/nano/103/results/publications-new.html

    • Related Report
      2012 Annual Research Report
  • [Remarks]

    • URL

      http://seika-kokai.nict.go.jp/jp/research/ResearchDetailsView.do;jsessionid=8FF0F6DBE467F8CB23D4B0E2541C1E34?search_psn_key=sjA2td7VXrc%3D

    • Related Report
      2011 Annual Research Report
  • [Remarks]

    • URL

      http://www-karc.nict.go.jp/103/paper/publications2010h22.html

    • Related Report
      2010 Annual Research Report
  • [Patent(Industrial Property Rights)] フォトニック結晶2011

    • Inventor(s)
      梶 貴博、山田俊樹、大友 明
    • Industrial Property Rights Holder
      梶 貴博、山田俊樹、大友 明
    • Filing Date
      2011-03-03
    • Related Report
      2012 Final Research Report
  • [Patent(Industrial Property Rights)] フォトニック結晶2011

    • Inventor(s)
      梶貴博、山田俊樹、大友明
    • Industrial Property Rights Holder
      情報通信研究機構
    • Industrial Property Number
      2011-046586
    • Filing Date
      2011-03-03
    • Related Report
      2010 Annual Research Report

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Published: 2010-08-23   Modified: 2019-07-29  

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