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Coherent Dynamics of Ultrafast Photoreactions in Proteins

Research Project

Project/Area Number 22750021
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Physical chemistry
Research InstitutionInstitute for Laser Technology

Principal Investigator

TANIGUCHI Seiji  (財)レーザー技術総合研究所, レーザーバイオ化学研究チーム, 研究員 (00342725)

Project Period (FY) 2010 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2010: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywords分子分光 / 光化学 / 過渡吸収
Research Abstract

In this study, we have investigated initial photo-reaction processes of photo-active proteins which shows remarkable response to light irradiation by transient absorption measurement using ultrashort pulse laser with 7.5 femtosecond(10^-15 second) width at time. As a result, we succeeded in the observation of the vibration modes of the chromophoric molecule in the photo-excited state of protein(PYP) which functioned as the eyes of the photosynthetic bacteria for the first time and elucidated that the structure of the protein causes ultrafast isomerization by evoking specific vibration modes of the chromophore.

Report

(3 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report
  • Research Products

    (22 results)

All 2012 2011 2010 Other

All Journal Article (7 results) (of which Peer Reviewed: 7 results) Presentation (12 results) Remarks (3 results)

  • [Journal Article]2012

    • Author(s)
      H. Chosrowjan, S. Taniguchi, T. Wongnate, J. Sucharitakul, P. Chaiyen, F. Tanaka
    • Journal Title

      Journal of Photochemistry and Photobiology A : Chemistry

      Volume: Vol.234 Pages: 44-48

    • DOI

      10.1016/j.jphotochem.2011.11.013

    • Related Report
      2011 Annual Research Report 2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] コスロービアンハイク、フェムト秒時間分解蛍光顕微鏡による光活性蛋白質微結晶の蛍光ダイナミクス2011

    • Author(s)
      谷口誠治
    • Journal Title

      レーザー研究

      Volume: 39巻 Pages: 931-937

    • URL

      http://www.lsj.or.jp/laser/

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Photoinduced ET in WT/Mutatedτ=212fsτ=210fs図7観測されたp-クマル酸の面内振動モードν13 modeν25 modeν36 mode FD from D. Vulgaris Strain Miyazaki : Energy Gap Law2011

    • Author(s)
      K. Lugsanangarm, S. Pianwanit, F. Tanaka, H. Chosrowjan, S. Taniguchi, N. Mataga
    • Journal Title

      Journal of Photochemistry and Photobiology A:Chemisrty

      Volume: Vol.219 Issue: 1 Pages: 32-41

    • DOI

      10.1016/j.jphotochem.2011.01.013

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] フェムト秒時間分解蛍光顕微鏡による光活性蛋白質微結晶の蛍光ダイナミクス2011

    • Author(s)
      谷口誠治
    • Journal Title

      レーザー研究

      Volume: 39 Pages: 931-937

    • NAID

      10030152055

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Photoinduced ET in WT/Mutated FD from D.Vulgaris Strain Miyazaki : Energy Gap Law2011

    • Author(s)
      Seiji Taniguchi
    • Journal Title

      Journal of Photochemistry and Photobiology A : Chemistry

      Volume: Vol.218 Pages: 32-47

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Effects of the Disappearance of One Charge on Ultrafast Fluorescence Dynamics of the FMN Binding Protein2010

    • Author(s)
      H. Chosrowjan, S. Taniguchi, N. Mataga, T. Nakanishi, Y. Haruyama, S. Sato, M. Kitamura, T. Tanaka
    • Journal Title

      J Phys. Chem. B

      Volume: vol.114 Issue: 18 Pages: 6175-6182

    • DOI

      10.1021/jp912137s

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Effects of the Disappearance of One Charge on Ultrafast Fluorescence Dynamics of the FMN Binding Protein2010

    • Author(s)
      Seiji Taniguchi
    • Journal Title

      The Journal of Physical Chemistry B.

      Volume: Vol.114 Pages: 6175-6182

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Presentation] ピロリ菌由来フラボドキシンの超高速光誘起反応ダイナミクス2012

    • Author(s)
      谷口誠治、コスロービアンハイク、田中文夫、荻野公美子、中西猛、北村昌也
    • Organizer
      日本化学会第92春季年会
    • Place of Presentation
      慶応大学
    • Year and Date
      2012-03-26
    • Related Report
      2011 Final Research Report
  • [Presentation] 中鎖アシルCoAデヒドロゲナーゼ(MCAD)の光誘起電子移動の観測と理論解析:正常領域における電子移動過程2012

    • Author(s)
      コスロービアンハイク、谷口誠治、中島信昭、K. Lugasanangarm, S. Pianvanit、S. Kokpol、N. Nunthaboot、田中文夫
    • Organizer
      日本化学会第92春季年会
    • Place of Presentation
      慶応大学
    • Year and Date
      2012-03-26
    • Related Report
      2011 Final Research Report
  • [Presentation] ピロリ菌由来フラボドキシンの超高速光誘起反応ダイナミクス2012

    • Author(s)
      谷口誠治
    • Organizer
      日本化学会第92春季年会
    • Place of Presentation
      慶応大学矢上、日吉キャンパス
    • Year and Date
      2012-03-26
    • Related Report
      2011 Annual Research Report
  • [Presentation] sub-10fsポンプ-プローブ測定による光活性黄色タンパク質(PYP)の光初期異性化ダイナミクス2011

    • Author(s)
      谷口誠治、コスロービアンハイク、小林孝嘉、Jin Liu、今元泰
    • Organizer
      2011年光化学討論会
    • Place of Presentation
      宮崎市河畔コンベンションエリア
    • Year and Date
      2011-09-06
    • Related Report
      2011 Annual Research Report 2011 Final Research Report
  • [Presentation] セリンヒドロキシメチル転移酵素(SHMT)の超高速蛍光ダイナミクス2011

    • Author(s)
      コスロービアンハイク、谷口誠治、田中文夫、P. Chaiyen、T. Wongnate
    • Organizer
      2011年光化学討論会
    • Place of Presentation
      宮崎市河畔コンベンションエリア
    • Year and Date
      2011-09-06
    • Related Report
      2011 Final Research Report
  • [Presentation] sub-10fs過渡吸収測定による光活性黄色タンパク質(PYP)の光初期異性化ダイナミクス2011

    • Author(s)
      谷口誠治、コスロービアンハイク、又賀〓、小林孝嘉、Jin Liu、今元泰
    • Organizer
      日本化学会第91春季年会
    • Place of Presentation
      神奈川大学
    • Year and Date
      2011-03-28
    • Related Report
      2011 Final Research Report
  • [Presentation] セリンヒドロキシメチル転移酵素(SHMT)の超高速蛍光ダイナミクス2011

    • Author(s)
      コスロービアンハイク、谷口誠治、又賀〓、P. Chaiyen,田中文夫
    • Organizer
      日本化学会第91春季年会
    • Place of Presentation
      神奈川大学
    • Year and Date
      2011-03-28
    • Related Report
      2011 Final Research Report
  • [Presentation] sub-10fs 過渡吸収測定による光活性黄色タンパク質(PYP)の光初期異性化ダイナミクス2011

    • Author(s)
      谷口誠治
    • Organizer
      日本化学会第90春季年会
    • Place of Presentation
      神奈川大学(神奈川大学)
    • Year and Date
      2011-03-28
    • Related Report
      2010 Annual Research Report
  • [Presentation] Fluorescence dynamics and Photoconductivity effect in PYP single crystals2011

    • Author(s)
      H. Chosrowjan, S. Taniguchi, Y. Imamoto
    • Organizer
      The Madrid Conference on Femtochemistry(FEMTO10)
    • Place of Presentation
      Spain
    • Related Report
      2011 Final Research Report
  • [Presentation] HPA(p-hydroxyphenyl-acetate)水酸化酵素の超高速蛍光ダイナミクス2010

    • Author(s)
      谷口誠治
    • Organizer
      2010年光化学討論会
    • Place of Presentation
      千葉大学(千葉県)
    • Year and Date
      2010-09-09
    • Related Report
      2010 Annual Research Report
  • [Presentation] HPA(p-hydroxyphenyl-acetate)水酸化酵素の超高速蛍光ダイナミクス2010

    • Author(s)
      谷口誠治、コスロービアンハイク、又賀〓、T. Phongsak、P. Chaiyen、田中文夫
    • Organizer
      2010年光化学討論会
    • Place of Presentation
      千葉大学
    • Year and Date
      2010-09-08
    • Related Report
      2011 Final Research Report
  • [Presentation] ピラノース2-オキシダーゼ(P2O)とその変異体の超高速蛍光ダイナミクス:グルコースおよびアセテート存在下での光過程2010

    • Author(s)
      コスロービアンハイク、谷口誠治、又賀〓、T. Phongsak、P. Chaiyen、田中文夫
    • Organizer
      2010年光化学討論会
    • Place of Presentation
      千葉大学
    • Year and Date
      2010-09-08
    • Related Report
      2011 Final Research Report
  • [Remarks] 谷口誠治、Sub-10fsレーザーパルスを用いたタンパク質の超高速光反応の計測、(財)レーザー技術総合研究所機関誌レーザークロスNo.283

    • URL

      http://www.ilt.or.jp

    • Related Report
      2011 Final Research Report
  • [Remarks]

    • URL

      http://www.ilt.or.jp/event/lasercross-paper/no284.pdf

    • Related Report
      2011 Annual Research Report
  • [Remarks]

    • URL

      http://www.ilt.or.jp/

    • Related Report
      2010 Annual Research Report

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Published: 2010-08-23   Modified: 2016-04-21  

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