Protein confomational dynamics probed by plasmon-coupling of nanoparticle
| Project/Area Number |
22750064
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Analytical chemistry
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| Research Institution | Akita University |
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Principal Investigator |
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| Project Period (FY) |
2010 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2010: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
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| Keywords | 分析化学 / ナノバイオ / プラズモンカップリング / 金ナノ粒子 / 一分子計測 / タンパク質 |
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| Research Abstract |
A measurement technique for reaction dynamics of proteins by an electronic coupling of localized surface plasmon resonance (LSPR) of individual gold nanoparticle was exploited. An apparatus which consists of dark-field microscope equipped with high-sensitive CMOS camera was constructed and employed for the measurements. A plasmon-coupling between single pairs of gold nanoparticles, which were modified with some proteins, was examined. The Light-scattering signal attribute to plasmon-coupling was digitally modulated with the observation time, which indicates the observed phenomena correspond to single-molecule events.
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Report
(3 results)
Research Products
(34 results)
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[Journal Article] Nondestructive estimation of strength deterioration in photovoltaic back sheets using a portable near infrared spectrometer2012
Author(s)
Li, H., Kikuchi, R., Kumagai, M., Amano, T., Tang, H., Lin, J.M., Fujiwara, K., Ogawa, N.
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Journal Title
Solar Energy Materials and Solar Cells
Volume: 101
Pages: 166-169
DOI
Related Report
Peer Reviewed
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[Journal Article] Nondestructive estimation of strength deterioration in photovoltaic backsheets using a portable near infrared spectrometer2011
Author(s)
Li, H., Kikuchi, R., Kumagai, M., Amano, T., Tang, H., Lin, J.-M., Fujiwara, K., Ogawa, N.
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Journal Title
Solar Energy Materials and Solar Cells
Volume: Vol.101
Pages: 166-169
Related Report
Peer Reviewed
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[Journal Article] Gentamicin inhibits HSP70-assisted protein folding by interfering with substrate recognition2010
Author(s)
Yamamoto, S., Nakano, S., Owari, K., Fuziwara, K., Ogawa, N., Otaka, M., Kubota, H., Itoh, H.
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Journal Title
FEBS Letters
Volume: Vol.584,No.4
Pages: 645-651
Related Report
Peer Reviewed
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[Presentation] Measurement of pH of Low Ionic Strength Solutions Using a Combination-type Glass pH Electrode Equipped with Ionic Liquid Salt Bridge2011
Author(s)
Kudo, Y., Kikuchi, R., Fujiwara, K., Shibata, M., Sakaida, H., Kakiuchi, T., Ogawa, N.
Organizer
IUPAC International Congress on Analytical Sciences 2011 (ICAS2011)
Place of Presentation
京都国際会議場
Year and Date
2011-05-23
Related Report
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[Presentation] Resonant light scattering imaging of protein2010
Author(s)
Fujiwara, K., Sato, K., Yatsurugi, Y., Yamamoto, S., Itoh, H., Ogawa, N.
Organizer
functionalized gold nanoparticle in a biological cell Pacifichem2010
Place of Presentation
ハワイコンベンションセンター
Year and Date
2010-12-17
Related Report
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[Presentation] Targeting of an intracellular protein by confocal2010
Author(s)
Sato, K., Fujiwara, K., Yatsurugi, Y., Yamamoto, S., Itoh, H., Ogawa, N.
Organizer
light scattering imaging of antibody-tagged gold nanoparticle Pacifichem2010
Place of Presentation
ハワイコンベンションセンター
Year and Date
2010-12-17
Related Report
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