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Development of functional cell array for long-term survival and recovery of tissues

Research Project

Project/Area Number 22750066
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Analytical chemistry
Research InstitutionThe University of Tokyo

Principal Investigator

YOSHIMOTO Keitaro  東京大学, 大学院・総合文化研究科, 准教授 (60392172)

Project Period (FY) 2010 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2011: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2010: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Keywords細胞分離 / 細胞培養皿 / 核酸 / アプタマー / 磁性粒子 / 細胞培養 / 三次元培養
Research Abstract

In order to develop the new method for cell recovery, a high-performance magnetic beads for biotinyl-compounds recovery was developed. In addition, assesment of cell attachemenct on DNA immobilized surface, spheroid culture of primary hepatocytes, and co-culture system of cancer spheroid were demonstrated.

Report

(3 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report
  • Research Products

    (13 results)

All 2012 2011 2010 Other

All Journal Article (4 results) (of which Peer Reviewed: 2 results) Presentation (9 results)

  • [Journal Article] Long-term survival and functional maintenance of hepatocytes by using a microfabricated cellarray2012

    • Author(s)
      Ikeda, Y., Jomura, T., Horiuchi, U., Saeki, J., Yoshimoto, K., Ikeya, T., Nagasaki, Y
    • Journal Title

      Colloids and Surfaces B : Biointerfaces

      Pages: 97-100

    • Related Report
      2011 Final Research Report
  • [Journal Article] Improvement of the thermal stability of streptavidin immobilized on magnetic beads by the construction of a mixed-poly(ethylene glycol) tethered-chain Layer2011

    • Author(s)
      Kubota, M., Yoshimoto, K., Yuan, X., Nagasaki, Y
    • Journal Title

      Polymer Journal

      Volume: 43 Pages: 493-496

    • NAID

      10028221047

    • Related Report
      2011 Final Research Report
  • [Journal Article] Improvement of the Thermal Stability of Streptavidin Immobilized on Magnetic Beads by the Construction of a Mixed-poly(ethylene glycol) Tethered-chain Layer2011

    • Author(s)
      Kubota, M., Yoshimoto, K., Yuan, X.,Nagasaki,Y.
    • Journal Title

      Polymer Journal

      Volume: 43 Pages: 493-496

    • NAID

      10028221047

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Long-termsurvival and functionalmaintenance of hepatocytes by using amicrofabricatedcellarray

    • Author(s)
      Ikeda Y., Jomura T., Horiuchia U., Saekia J., Yoshimoto, K., Ikeya T., Nagasaki, Y
    • Journal Title

      Colloids and Surfaces B : Biointerfaces

      Volume: (掲載決定)

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Presentation] 高分子修飾表面の界面力-生体高分子・細胞に与える不思議な環境2012

    • Author(s)
      吉本敬太郎
    • Organizer
      第12回表面力セミナー
    • Place of Presentation
      東北大学
    • Year and Date
      2012-03-03
    • Related Report
      2011 Final Research Report
  • [Presentation] 3D Cell Co-culture System on Hydrogel Micro-Patterned Surface2011

    • Author(s)
      Keitaro Yoshimoto
    • Organizer
      MRS Fall Meeting
    • Place of Presentation
      Boston, USA
    • Year and Date
      2011-11-29
    • Related Report
      2011 Final Research Report
  • [Presentation] 3D Cell Co-culture System on Hydrogel Micro-Patterned Surface2011

    • Author(s)
      吉本敬太郎
    • Organizer
      MRS Fall Meeting
    • Place of Presentation
      ボストン(アメリカ)(招待講演)
    • Year and Date
      2011-11-29
    • Related Report
      2011 Annual Research Report
  • [Presentation] 培養細胞の構造制御と高機能化-細胞も3Dの時代2011

    • Author(s)
      吉本敬太郎
    • Organizer
      第12回機能構造と分析化学シンポジウム
    • Place of Presentation
      東北大学
    • Year and Date
      2011-11-19
    • Related Report
      2011 Final Research Report
  • [Presentation] Fab'/mixed-PEG co-immobilized gold surface-The role of a PEG tethered layer in molecular recognition events-2011

    • Author(s)
      Yukio Nagasaki, Motohiko Nishio, Keitaro Yoshimoto
    • Organizer
      ICTF15
    • Place of Presentation
      Fukuoka
    • Year and Date
      2011-11-08
    • Related Report
      2011 Final Research Report
  • [Presentation] 分子認識の選択性を高めるソフト界面設計2011

    • Author(s)
      吉本敬太郎,長崎幸夫
    • Organizer
      日本分析化学会第60年会
    • Place of Presentation
      名古屋大学
    • Year and Date
      2011-09-14
    • Related Report
      2011 Final Research Report
  • [Presentation] Structural Analysis and Molecular Recognition Behavior of Bio-macromolecular/Polymer Hybrid Interface2011

    • Author(s)
      Keitaro Yoshimoto
    • Organizer
      ICAS2011
    • Place of Presentation
      Kyoto International Conference Center, Kyoto, Japan
    • Year and Date
      2011-05-24
    • Related Report
      2011 Final Research Report
  • [Presentation] 生体高分子構造の空間制御に基づく高性能診断法の開発2010

    • Author(s)
      吉本敬太郎
    • Organizer
      日本分析化学会第59年会
    • Place of Presentation
      東北大学
    • Year and Date
      2010-09-16
    • Related Report
      2011 Final Research Report
  • [Presentation] 胎生肝細胞の長期培養と分化誘導法2010

    • Author(s)
      吉本敬太郎,小島綾太,長崎幸夫
    • Organizer
      第59回高分子学会年次大会
    • Place of Presentation
      横浜
    • Year and Date
      2010-05-28
    • Related Report
      2011 Final Research Report

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Published: 2010-08-23   Modified: 2016-04-21  

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