Development of Simultaneous detection for Bacteria, Virus, and Toxin using Microchip with Ion-channel Sensor

• Project/Area Number: 22750068
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Analytical chemistry
• Research Institution: University of Toyama
• Principal Investigator: HIDEKI Kuramitz 富山大学, 大学院・理工学研究部(理学), 准教授 (70397165)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2011: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2010: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
• Keywords: 電気化学分析 / 大腸菌 / 毒素タンパク質 / マイクロ流路 / 磁性粒子 / 細菌 / ウイルス

Research Abstract

The aim of this study is the development for the microchip devise with ion-channel sensor for simultaneous detection of bacteria, virus, and protein toxin. The development of detecting units for cholera toxin, MS2, and E-coli were successively achieved by constructing the antibody assembled membrane that acts as the ion-channel for ferrocyanide on the electrode surface. As the separating units, the separation of E-coli bound/unbound magnetic microbeads(1μm) was investigated using the pinched flow fractionation microchip. The determination of 5×10^2 cfu/mL E-coli was achieved by this method.

Research Products

• [Journal Article] (2012)
  • Author(s): H. Kuramitz, Y. Mawatari, M. Ikeuchi, O. Kutomi, N. Hata, S. Taguchi, and K. Sugawara
  • Journal Title: Analytical Sciences Volume: 28 Pages: 77-81
• [Journal Article] (2012)
  • Author(s): M. Fukushima, Y. Mizutani, S. Maeno, Q. Zhu, H. Kuramitz, and S. Nagao
  • Journal Title: Molecules Volume: 17 Pages: 48-60
• [Journal Article] Multiplexed assay for proteins based on sequestration electro chemistry using the protein binding electroactive magnetic microbeads (2012)
  • Author(s): Hideki Kuramitz, Yoko Mawatari, Mariwo Ikeuchi, Osamu Kutomi, Noriko Hata, Shigeru Taguchi, Kazuharu Sugawara
  • Journal Title: Analytical Sciences Volume: 28 Pages: 77-81
  • Peer Reviewed
• [Journal Article] Magnetic microbead-based enzyme immunoassay for ovalbum in using hydrodynamic voltammetry and fluorometric detection (2012)
  • Author(s): Hideki Kuramitz, H.Brian Halsall, William R.Heineman
  • Journal Title: Analytical Methods Volume: (In press) Issue: 6 Pages: 1783-1783
  • DOI: 10.1039/c2ay05738g
  • Peer Reviewed
• [Journal Article] (2011)
  • Author(s): K. Sazawa, M. Tachi, T. Wakimoto, T. Kawakami, N. Hata, S. Taguchi and H. Kuramitz
  • Journal Title: International Journal of Environmental Research and Public Health Volume: 8 Pages: 1655-1670
• [Journal Article] (2011)
  • Author(s): H. Kuramitz, S. Miyagaki, E. Ueno, N. Hata, S. Taguchi, and K. Sugawara
  • Journal Title: Analyst Volume: 136 Pages: 2373-2378
• [Journal Article] Binding assay for cholera toxin based on sequestration electr ochemistry using lactose labeled with an electroactive compound (2011)
  • Author(s): Hideki Kuramitz, Shun Miyagaki, Eiji Ueno, Noriko Hata, Shigeru Taguchi, Kazuharu Sugawara
  • Journal Title: Analyst Volume: 136 Pages: 2373-2378
  • Peer Reviewed
• [Journal Article] The evaluation for alterations of DOM components from upstream to downstream flow of rivers in Toyama (Japan) using three-dimensional excitation-emission matrix fluorescence spectroscopy (2011)
  • Author(s): Kazuto Sazawa, Masaki Tachi, Takatoshi Wakimoto, Takanori Kawakami, Noriko Hata, Shigeru Taguchi, Hideki Kuramitz
  • Journal Title: International Journal of Environmental Research and Public Health Volume: 8 Issue: 5 Pages: 1655-1670
  • DOI: 10.3390/ijerph8051655
  • Peer Reviewed
• [Journal Article] (2010)
  • Author(s): 田口茂, 藤井絢子, 起孝志, 倉光英樹, 波多宣子
  • Journal Title: 分析化学 Volume: 59 Pages: 1133-1136
• [Journal Article] Pattern of oxidation products derived from tetrobromobisphenol A in a catalytic system comprised of iron (III)-tetrakis(p-sulfophenyl)porphyrin, KHSO_5 and humic acids (2010)
  • Author(s): M.Fukushima, Y.Ishida, S.Shigematsu, H.Kuramitz, S.Nagao
  • Journal Title: Chemosphere Volume: 80 Pages: 860-865
  • Peer Reviewed
• [Presentation] IUPAC International Congress on Analytical Sciences 2011 (2011)
  • Author(s): K. Sazawa, H. Kuramitz, N. Hata, S. Taguchi, and M. Fukushima
  • Organizer: ICAS2011
  • Place of Presentation: Kyoto, Japan
• [Presentation] (2011)
  • Author(s): M. Ikeuchi, H. Kuramitz, N. Hata, S. Taguchi, and K. Sugawara
  • Organizer: 62^<nd> Annual Meeting of the International Society of Electrochemistry
  • Place of Presentation: Niigata, Japan
• [Presentation] (2011)
  • Author(s): T. Wakimoto, K. Sazawa, N. Hata, S. Taguchi, and H. Kuramitz
  • Organizer: 3rd International Workshop on "Wild Fire and Carbon Management in Peat-Forest in Indonesia"
  • Place of Presentation: Palangka Raya, Indonesia
• [Presentation] (2011)
  • Author(s): K. Sazawa, W. Ohura, Y. Furuhashi, N. Hata, S. Taguchi, M. Fukushima, and H. Kuramitz
  • Organizer: The 4^<th> IWA-ASPIRE Conference & Exhibition
  • Place of Presentation: Tokyo, Japan
• [Presentation] (2011)
  • Author(s): T. Okazaki, W. Wang, H. Kuramitz, N. Hata, and S. Taguchi
  • Organizer: The 4^<th> IWA-ASPIRE Conference & Exhibition
  • Place of Presentation: Tokyo, Japan
• [Presentation] (2011)
  • Author(s): N. Hata, T. Nakajima, K. Nakagawa, M. Nakamura, H. Kuramitz, S. Taguchi, and H. Chiba
  • Organizer: The 4^<th> IWA-ASPIRE Conference & Exhibition
  • Place of Presentation: Tokyo, Japan
• [Presentation] (2011)
  • Author(s): N. Hata, S. Terashima, R. Yasui, H. Kuramitz, and S. Taguchi
  • Organizer: The 4^<th> IWA-ASPIRE Conference & Exhibition
  • Place of Presentation: Tokyo, Japan
• [Presentation] (2011)
  • Author(s): S. Taguchi, K. Murai, Y. Hayakawa, S. Tamizu, M. Kuwata, Y. Katayama, N. Hata, and H. Kuramitz
  • Organizer: The 4^<th> IWA-ASPIRE Conference & Exhibition
  • Place of Presentation: Tokyo, Japan
• [Presentation] (2011)
  • Author(s): 鈴木智代・長尾誠也・倉光英樹・落合伸也・徳成武勇・山本政儀
  • Organizer: 第27回日本腐植物質学会講演会
  • Place of Presentation: 金沢大学
• [Presentation] (2011)
  • Author(s): 福嶋正巳・水谷祐介・前野翔平・倉光英樹・長尾誠也
  • Organizer: 第27回日本腐植物質学会講演会
  • Place of Presentation: 金沢大学
• [Presentation] (2011)
  • Author(s): 脇本孝俊・佐澤和人・倉光英樹・波多宣子・田口茂・福嶋正巳・田中俊逸
  • Organizer: 第27回日本腐植物質学会講演会
  • Place of Presentation: 金沢大学
• [Presentation] (2011)
  • Author(s): 桑田真実・村居景太・片山祐貴・倉光英樹・波多宣子・田口茂
  • Organizer: 日本分析化学会第60年会
  • Place of Presentation: 名古屋大学
• [Presentation] (2011)
  • Author(s): 水名健太・波多宣子・倉光英樹・田口茂・中山慶子・山本保・高村禅
  • Organizer: 日本分析化学会第60年会
  • Place of Presentation: 名古屋大学
• [Presentation] (2011)
  • Author(s): 浅岡雅・廣上永子・倉光英樹・波多宣子・田口茂・川上貴教
  • Organizer: 日本分析化学会第60年会
  • Place of Presentation: 名古屋大学
• [Presentation] One-step Binding Assay by Magnetic Microbead-based Seque stration Electrochemistry for Cholera Toxin (2011)
  • Author(s): Mariwo Ikeuchi, Hideki Kuramitz, Noriko Hata, Shigeru Taguchi, Kazuharu Sugawara
  • Organizer: 62^<nd> Annual Meeting of the International Society of Electrochemistry
  • Place of Presentation: 新潟県新潟市
• [Presentation] The protein sensing by sequestration electrochemistry and its applications (2010)
  • Author(s): H.Kuramitz
  • Organizer: 3rd International Conference for Young Chemists(ICYC 2010)
  • Place of Presentation: Penang, Malaysia
  • Year and Date: 2010-06-24
• [Presentation] (2010)
  • Author(s): H. Kuramitz and K. Sugawara
  • Organizer: 3rd International Conference for Young Chemists
  • Place of Presentation: Penang, Malaysia
• [Presentation] (2010)
  • Author(s): K. Sazawa, H. Kuramitz, N. Hata, and S. Taguchi
  • Organizer: The 17^<th> Asian Symposium on Ecotechnology(ASET17)
  • Place of Presentation: Toyama, Japan
• [Presentation] (2010)
  • Author(s): H. Kuramitz, K. Sazawa, N. Hata, and S. Taguchi
  • Organizer: 2nd International Workshop on "Wild Fire and Carbon Management in Peat-Forest in Indonesia"
  • Place of Presentation: Palangka Raya, Indonesia
• [Presentation] (2010)
  • Author(s): K. Sazawa, H. Kuramitz, N. Hata, and S. Taguchi
  • Organizer: 2nd International Workshop on "Wild Fire and Carbon Management in Peat-Forest in Indonesia"
  • Place of Presentation: Palangka Raya, Indonesia
• [Presentation] (2010)
  • Author(s): W. R. Heineman, H. B. Halsall, C. J. Seliskar, H. Kuramitz
  • Organizer: The 61^<st> Annual Meeting of the International Society of Electrochemistry
  • Place of Presentation: Nice, France
• [Book] 化学反応による定性分析, 2. 1.化学反応による検出, 2. 1. 1.陽イオン, 2. 1. 2.陰イオン「分析化学便覧(第6版)」 (2011)
  • Author(s): 倉光英樹
  • Publisher: 日本分析化学会編,丸善
• [Book] 分析化学便覧(第6版) (2011)
  • Author(s): 倉光英樹(共著)
  • Total Pages: 880
  • Publisher: 丸善
• [Remarks]
  • URL: http://www.sci.u-toyama.ac.jp/env/kuramitz/indexJP.html
• [Remarks]
  • URL: http://www.sci.u-toyama.ac.jp/env/kuramitz/indexJP.html