| Project/Area Number |
22750068
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Analytical chemistry
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| Research Institution | University of Toyama |
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Principal Investigator |
HIDEKI Kuramitz 富山大学, 大学院・理工学研究部(理学), 准教授 (70397165)
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| Project Period (FY) |
2010 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2011: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2010: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
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| Keywords | 電気化学分析 / 大腸菌 / 毒素タンパク質 / マイクロ流路 / 磁性粒子 / 細菌 / ウイルス |
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| Research Abstract |
The aim of this study is the development for the microchip devise with ion-channel sensor for simultaneous detection of bacteria, virus, and protein toxin. The development of detecting units for cholera toxin, MS2, and E-coli were successively achieved by constructing the antibody assembled membrane that acts as the ion-channel for ferrocyanide on the electrode surface. As the separating units, the separation of E-coli bound/unbound magnetic microbeads(1μm) was investigated using the pinched flow fractionation microchip. The determination of 5×10^2 cfu/mL E-coli was achieved by this method.
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