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Development of a highly sensitive visual immunoassay method based on DNA amplification and gold nanoparticles.

Research Project

Project/Area Number 22750076
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Analytical chemistry
Research InstitutionJapan Women's University

Principal Investigator

SATO Kae  日本女子大学, 理学部, 准教授 (40373310)

Project Period (FY) 2010 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2011: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2010: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
KeywordsDNA / RCA / ビーズ / 金ナノ粒子 / 免疫分析
Research Abstract

Immunoassay is one of the most important analytical methods because of its specificity. To simplify the analysis, a new method based on highly sensitive visual test is strongly required. We established fundamental technologies for immunoassays using enzymes and conjugates of inorganic materials (gold nanoparticle) and biomolecules (DNA). We investigated to develop solid substrates used as efficient reaction fields, make DNA-modified antibodies, and optimize the reactions of gold nanoparticles and amplified products of DNA samples.

Report

(3 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report
  • Research Products

    (5 results)

All 2011 2010 Other

All Presentation (5 results)

  • [Presentation] Counting Single DNA Molecule By,On-Bead Rolling Circle Amplification For Quantitative Analyses2011

    • Author(s)
      R Ishi1,N Sasaki,K Sato,K Mawatari, M Nilsson, T Kitamori, K Sato
    • Organizer
      microTAS2011
    • Place of Presentation
      シアトル
    • Related Report
      2011 Final Research Report
  • [Presentation] Single DNA Molecule Detection by on-Bead Rolling Circle Amplification Using Microchip for Efficient Detection2010

    • Author(s)
      K. Sato, Y. Kitamura, N. Sasaki, K.Sato, K. Mawatari, M. Nilsson, T. Kitamori
    • Organizer
      microTAS 2010
    • Place of Presentation
      オランダ、フロー ニンゲン
    • Related Report
      2011 Final Research Report
  • [Presentation] マイクロビーズを用いたpadlock/RCA法による単一DNA分子カウンティング2010

    • Author(s)
      佐藤香枝・北村友理・佐々木直樹・Nilsson Mats
    • Organizer
      日本分析化学会第59年会
    • Place of Presentation
      東北大学
    • Related Report
      2011 Final Research Report 2010 Annual Research Report
  • [Presentation] Single DNA Molecule Detection by on-Bead Rolling Circle Amplification Using Microchip for Efficient Detection2010

    • Author(s)
      K.Sato, Y.Kitamura, N.Sasaki, K.Sato, K.Mawatari, M.Nilsson, T.Kitamori
    • Organizer
      microTAS 2010
    • Place of Presentation
      オランダ、フローニンゲン
    • Related Report
      2010 Annual Research Report
  • [Presentation] COUNTING SINGLE DNA MOLECULE BY ON-BEAD ROLLING CIRCLE AMPLIFICATION FOR QUANTITATIVE ANALYSES

    • Author(s)
      Reina Ishi1, Naoki Sasaki, Kiichi Sato, Kazuma Mawatari, Mats Nilsson, Takehiko Kitamori, Kae Sato
    • Organizer
      microTAS2011
    • Place of Presentation
      シアトル
    • Related Report
      2011 Annual Research Report

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Published: 2010-08-23   Modified: 2016-04-21  

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