Separation of bio materials using photo-responsive reverse micelles

• Project/Area Number: 22750135
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Functional materials chemistry
• Research Institution: Nara National College of Technology
• Principal Investigator: UDA Ryoko 奈良工業高等専門学校, 物質化学工学科, 准教授 (90321463)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2012: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2011: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2010: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 膜・集合体 / 光応答 / 逆ミセル / マラカイトグリーン

Research Abstract

A photoresponsive compound which turns to be a cationic surfactant by irradiation was used as a functional compound. Anionic reverse micelle is disrupted by irradiation of the photoresponsive compound solubilized in anionic surfactant-water- chloroform mixture. The disruption was investigated by using proton nuclear magnetic resonance spectroscopy. Furthermore, the photoinduced disruption of reverse micelle is shown to release enzyme incorporated in the interior of the photoresponsive reverse micelle.

Research Products

• [Journal Article] Disruption ofreverse micelles and release of trappedribonuclease A photochemically inducedby Malachite Green leuconitrilederivative (2011)
  • Author(s): Ryoko M. Uda, Tsuyoshi Nishikawa,Yoshitsugu Morita
  • Journal Title: Journal of Colloid andInterface Science Volume: 355巻 Pages: 448-452
  • Peer Reviewed
• [Journal Article] Disruption of reverse micelles and release of trapped ribonuclease A photochemically induced by Malachite Green leuconitrile derivative (2011)
  • Author(s): Ryoko M.Uda, Tsuyoshi Nishikawa, Yoshitsugu Morita
  • Journal Title: Journal of Colloid and Interface Science Volume: 355 Pages: 448-452
  • Peer Reviewed
• [Presentation] Photosensitive Separationof Proteins by Reverse MicellesContaining Malachite Green Derivative (2011)
  • Author(s): Ryoko M. Uda
  • Organizer: IUPAC International Congress onAnalytical Sciences 2011
  • Place of Presentation: Kyoto, Japan
  • Year and Date: 2011-05-25
• [Presentation] Photosensitive Separation of Proteins by Reverse Micelles Containing Malachite Green Derivative (2011)
  • Author(s): Ryoko M.Uda
  • Organizer: IUPAC International Congress on Analytical Sciences 2011
  • Place of Presentation: Kyoto International Conference Center, Japan
  • Year and Date: 2011-05-25
• [Presentation] Photoresponsive Molecular ContainersBased on Self-Assembly of Amphiphilesand Ionizable Malachite GreenDerivative (2010)
  • Author(s): Ryoko M. Uda, Eri Hiraishi, TsuyoshiNishikawa, Yoshitsugu Morita
  • Organizer: 6th International Symposium on Organic Photochromism
  • Place of Presentation: Yokohama, Japan
  • Year and Date: 2010-10-19
• [Presentation] Photoresponsive Molecular Containers Based on Self-Assembly of Amphiphiles and Ionizable Malachite Green Derivative (2010)
  • Author(s): Ryoko M.Uda, Eri Hiraishi, Tsuyoshi Nishikawa, Yoshitsugu Morita
  • Organizer: 6^<th> International Symposium on Organic Photochromism
  • Place of Presentation: Yokohama, Japan
  • Year and Date: 2010-10-19