Development of the Artificial Oxidase Driven by Visible Light

• Project/Area Number: 22750152
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Chemistry related to living body
• Research Institution: The Institute of Physical and Chemical Research
• Principal Investigator: TAINAKA Kazuki 独立行政法人理化学研究所, 合成生物学研究グループ, 研究員 (80506113)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2010: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 光アンテナ / Ru(II)錯体修飾DNA / 可視光 / 光電流応答 / ピロロキノリンキノン / グルコース脱水素酵素 / PNA / 正孔輸送リレーユニット / オキシダーゼ / 補酵素 / グルコースデヒドロゲナーゼ / Ru(II)錯体 / DNA自己組織化膜 / 光誘起電子移動 / カソード光電流応答

Research Abstract

In this project, we developed a photoelectric transducer consist of Ru(II) complex as a light-harvesting antenna and DNA scaffold as a charge transporter. The DNA-modified films tethering Ru(II) complex showed cathodic photocurrent under visible light irradiation due to photoinitiated hole transport through DNA duplexes.

Research Products

• [Journal Article] Positional effects of phosphorylation on the stability and the morphology of tau-related amyloid fibrils (2012)
  • Author(s): Masafumi Inoue、Takashi Konno、Kazuki Tainaka Eiji Nakata Hiro-o Yoshida Takashi Morii
  • Journal Title: Biochemistry Volume: 51巻 Issue: 7 Pages: 1396-1406
  • DOI: 10.1021/bi201451z
  • Peer Reviewed
• [Journal Article] Generation of singletoxygen during photosensitized one-electronoxidation of DNA (2012)
  • Author(s): Yasuko Osakada, Mamoru Fujitsuka, Tetsuro Majima
  • Journal Title: Chemistry-A EuropeanJournal Volume: Vol.18 Issue: 4 Pages: 1060-1063
  • DOI: 10.1002/chem.201101964
  • Peer Reviewed
• [Journal Article] ICON probes: synthesis and DNA methylation typing. (2011)
  • Author(s): Tainaka K, Okamoto A.
  • Journal Title: Curr Protoc Nucleic Acid Chem. Volume: 8 Issue: 1
  • DOI: 10.1002/0471142700.nc0807s47
  • Peer Reviewed
• [Journal Article] Sequence Dependence of Excess Electron Transfer in DNA (2010)
  • Author(s): M. Fujitsuka, K. Kawai, T. Majima
  • Journal Title: J. Phys. Chem. B Volume: 114 Issue: 45 Pages: 14657-14663
  • DOI: 10.1021/jp1024685
  • Peer Reviewed
• [Journal Article] Design strategies of fluorescent biosensors based on biological macromolecular receptors. (2010)
  • Author(s): Tainaka K, Sakaguchi R, Hayashi H, Nakano S, Liew FF, Morii T.
  • Journal Title: Sensors (Basel). Volume: 10 Issue: 2 Pages: 1355-1376
  • DOI: 10.3390/s100201355
  • Peer Reviewed
• [Journal Article] An in vitro fluorescent sensor reveals intracellular Ins(1, 3, 4, 5) P4 dynamics in single cells (2010)
  • Author(s): T. Morii, et al
  • Journal Title: Angew Chem. Mt. Ed Volume: Vol.49 Issue: 12 Pages: 2150-2153
  • DOI: 10.1002/anie.200903951
  • Peer Reviewed
• [Patent(Industrial Property Rights)] 非天然型塩基を含むオリゴデオキシヌクレオチドの切断による5'末端リン酸化オリゴデオキシヌクレオチドの製造方法 (2012)
  • Inventor(s): 上田泰己、池田修司、田井中一貴
  • Industrial Property Rights Holder: 同上
  • Filing Date: 2012-04-02
• [Patent(Industrial Property Rights)] 非天然型塩基を含むオリゴデオキシヌクレオチドの切断による5'末端リン酸化オリゴデオキシヌクレオチドの製造方法 (2012)
  • Inventor(s): 上田泰己、池田修司、田井中一貴
  • Industrial Property Rights Holder: 同上
  • Filing Date: 2012-04-02
  • Overseas
• [Patent(Industrial Property Rights)] 非天然型塩基を含むオリゴデオキシヌクレオチドの切断による5’末端リン酸化オリゴデオキシヌクレオチドの製造方法 (2012)
  • Inventor(s): 上田泰己、池田修司、田井中一貴
  • Industrial Property Rights Holder: 上田泰己、池田修司、田井中一貴
  • Industrial Property Rights Type: 特許
  • Industrial Property Number: 2012-084249
  • Filing Date: 2012-04-02
• [Patent(Industrial Property Rights)] 非天然型塩基を含むオリゴデオキシヌクレオチドの切断による5’末端リン酸化オリゴデオキシヌクレオチドの製造方法 (2012)
  • Inventor(s): 上田泰己、池田修司、田井中一貴
  • Industrial Property Rights Holder: 上田泰己、池田修司、田井中一貴
  • Industrial Property Rights Type: 特許
  • Filing Date: 2012-04-02
  • Overseas