| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2010: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
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| Research Abstract |
We have developed multiplex fourth order Raman microscope for imaging of membrane proteins. We succeeded to measure fourth order Raman spectrum of micro DAST crystal which shows the peaks at 1170 cm^<-1>(ring breathing vibration of tosylate), 1347 cm^<-1>(molecular vibration of CH_3), and 1577 cm^<-1>(double bond vibration mode(C-C=C-C) between the two stilbazolium chromophores). Fourth order Raman image of DAST was obtained with microscopic resolution. Furthermore we observed polarization dependence of fourth order Raman spectrum of DAST and the possibility of the imaging of membrane proteins was confirmed.
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